Báo cáo khoa học: "Goal directed therapy: how long can we wait" doc

... mortality data in such an under-powered sample size. The interpretation of mortality rates is also confounded by the Commentary Goal directed therapy: how long can we wait? Anthony C Gordon 1 and ... min –1 m –2 . Mortality rates were similar in both groups. This was a well conducted randomised controlled study that importantly had an appropriately managed control group. It is, ho...

Ngày tải lên: 12/08/2014, 23:21

... Suttner Abstract Introduction: Several studies have shown that goal -directed hemodynamic and fluid optimization may result in improved outcome. However, the methods used were either invasive or had other limitations. ... a reduced LOS (nine vs. six days) and a reduced compl i- cation rate. The median POSSUM scores, however, were lower in this study (exp laining the shorter LOS), admin- istr...

Ngày tải lên: 13/08/2014, 20:21

... reply 800. doi:10.1186/cc8884 Cite this article as: Roche AM, Miller TE: Goal -directed or goal-misdirected – how should we interpret the literature? Critical Care 2010, 14:129. Roche and Miller ... adoption of these waveform-based technologies.  e big question is: what should we do, or how should we go about early GDT? We believe that carefully managed crystalloids, following...

Ngày tải lên: 13/08/2014, 20:21

... wild-type E1. In contrast, the reductive acetylation assay showed no significant changes for the mutants compared with wild-type E1. However, in the PDH assay, the catalytic activity fell to about ... experiment were carried out as described in detail elsewhere [35]. Results Multiple sequence alignment of E1 components of 2-OADH complexes No obvious sequence homology can be detected between...

Ngày tải lên: 20/02/2014, 23:20

... the variants displayed lower catalytic efficiencies than wild-type AAO, although some of the differences were not significant, taking into account the standard deviations. However, no efficiency decrease, ... residues potentially involved in AAO catalysis were selected after substrate docking and modified by site- directed mutagenesis. The different mutations were introduced in the aao cDNA by...

Ngày tải lên: 07/03/2014, 11:20

... important question there- fore is how many human-produced references are needed to achieve a certain level of performance. Do we really need hundreds of instances, or can we al- ready make informed ... the FN costs of Theune et al. (2010) are shown in Table 1. No significant differences were found, which sug- gests that k-means clustering, with k = 2, can be used as a more systematic...

Ngày tải lên: 07/03/2014, 22:20

... node. We can get at least one set of ordered nodes since we are treating an HDAG. In the case of , we can get , , , , , , . We can rewrite the recursive calculation formula in “for loops”, if we ... between pairs of objects. This means that we do not have to map texts to the feature vectors by explicitly representing them, as long as an efficient calculation for the inner p...

Ngày tải lên: 08/03/2014, 04:22

... structure along the lowest-energy mode, it was possible to gener- ate an ‘open’ conformation of the enzyme (Fig. 4). Next, two different systems were tested by the rosettadock protein–protein docking ... 000 decoys were produced for each of them. The analyzed systems were Gln134-restrained docking of both closed-MTGase–G-CSF and open-MTGase– G-CSF; Gln134-restrained docking means that we pe...

Ngày tải lên: 16/03/2014, 00:20

... none were visible on plates with 20 lgÆmL )1 TMP. Thirty colonies were selected from the plate with 2 lg ÆmL )1 TMP. Plasmids were isolated, and the genes corresponding to the truncated AMPP were ... solubility assay The intact AMPP as well as the C-terminal fragments of AMPP were expressed in the same manner. The genes were PCR amplified and cloned between the NdeI and EcoRI sites of the ......

Ngày tải lên: 23/03/2014, 07:20

... understand at the molecular level how KNOX transcription factors recognize their DNA target site. In this study, we analysed the interaction of the homeodomain of HAKN1, a sunflower class I KNOX protein ... them. Figure 1A shows an electrophoretic mobility shift assay performed with HAKN1 and oligonucleotide BS1 or variants containing changes at single positions (sequences shown in the rig...

Ngày tải lên: 23/03/2014, 13:20