Retrovirology Research BioMed Central Open Access A role for CD81 on the late steps of HIV-1 pdf
Retrovirology Research BioMed Central Open Access A role for CD81 on the late steps of HIV-1 pdf
... correspond to lyso-
somes. A partial co-localization of Gag and Env appeared
with the CD45 plasma membrane protein.
Quantification of Gag co-localization with tetraspanins
revealed that Gag was mainly ... in plasma membrane
invaginations [21]}. It appears that HIV-1 can accumulate
Partial inhibition of HIV-1 release using anti-tetraspanin antibodiesFigure 6
Partial inhibition o...
Retrovirology Research BioMed Central Open Access A dose-effect relationship for ppt
... performed with Sequence Navigator
Software.
Statistical analysis
SPSS statistical software version 11 was used for analyses.
The correlation of data was assessed by Spearman's Rho
nonparametric ... infected animals only after a prolonged period of
latency. From these data, it is possible to propose that infec-
tion duration and viral dose, which depends on both RT
and clonal...
Retrovirology Research BioMed Central Open Access A novel HIV-1 restriction factor that is potx
... [10]. A3 B, A3 DE, A3 F, and
A3 G contain two Zinc-binding motifs, while A3 A, A3 C,
and A3 H contain only one. A3 B, A3 DE, A3 F, and A3 G
inhibit HIV-1 replication to different degrees, whereas
A3 A and A3 C ... A3 G gene contained a R14Q
and A3 F contained a Q275E mutation. To test how these
mutations affected A3 G and A3 F, these genes were cloned
into pcDNA3.1 assa...
Retrovirology Research BioMed Central Open Access Isolation and characterization of a small ppsx
... core formation
not only results as a consequence of the proteolytic cleav-
age of p55 but also from substantial conformational
changes and rearrangements of the p24 [1] which is con-
nected to one ... the
chemically synthesized α-HGA further confirmed that the antiviral G-NH
2
-metabolite indeed was
α-HGA.
Conclusion: α-HGA has an unusually simple structure and a novel mecha...
Retrovirology Research BioMed Central Open Access HIV-1 TAR miRNA protects against apoptosis by ppt
... (Fig. 1A and 1B). Cloning analysis
recovered three clones of the 5' arm of the TAR miRNA
(TAR-5p) and 14 clones of the 3' arm (TAR-3p). The 5' end
of the TAR-5p miRNA appears to ... mediated by the TAR viral miRNA.
TAR miRNA alters apoptotic genes
The observation that the HIV-1 TAR miRNA is expressed
both in latent and in active infection suggests tha...
Retrovirology Research BioMed Central Open Access In vivo expression of the HBZ gene of HTLV-1 doc
... Murata K, Hayashibara T, Sugahara K, Uemura A, Yamaguchi T, Har-
asawa H, Hasegawa H, Tsuruda K, Okazaki T, Koji T, Miyanishi T,
Yamada Y, Kamihira S: A novel alternative splicing isoform of
human ... Y, Takamori A, Utsunomiya A, Kurimura M, Yamano Y,
Hishizawa M, Hasegawa A, Kondo F, Kurihara K, Harashima N,
Watanabe T, Okamura J, Masuda T, Kannagi M: Impaired Tax-spe-
cific T-cell r...
Retrovirology Research BioMed Central Open Access Recruitment of HIV-1 envelope occurs subsequent ppt
... region analyzed by
FRAP and the other one used as a fluorescence control.
A schematic illustration of an updated HIV-1 Env-mediated fusion modelFigure 7
A schematic illustration of an updated HIV-1 ... [26].
The dynamics of protein complexes at the cell surface are
determined by the organization, oligomerization state,
and interaction with the membrane lipids and oth...
Retrovirology Research BioMed Central Open Access Modulation of HIV-1 infectivity and cyclophilin ppsx
... (6-Fam)AGGACTCATGACCACAGTCCATGCCA(Tamra)
CypA
forward primer 5'- GGCCGCGTCTCCTTTGA
reverse primer 5'- AATCCTTTCTCTCCAGTGCTCAGA
Probe 5'- (6-Fam)TGCAGACAAGGTCCCAAAGACAGCAG(Tamra)
TRIM5α
forward primer 5'- TGCCTCTGACACTGACTAAGAAGATG
reverse ... TGCCTCTGACACTGACTAAGAAGATG
reverse primer 5'- GGGCTAAGGACTCATTCATTGG
Probe 5'- (6-Fam)AAGCTTTTCAACAGCCTTTCTATATCATCGTGTGAT...
Retrovirology Research BioMed Central Open Access APOBEC3G mRNA expression in exposed ppt
... GTATCT
AATAG3'), and JVPvifF2 (5'T GG AAAGG ACCAG
CAAAGCT3') and JVPvifR (CTAGGAAAATGTCTAACAGC
TT), respectively. We used High Fidelity Polimerase (Plat-
inum Taq DNA Polymerase ... specific for hA3G (NM 021822), hu
ApoB 3G F2 (CAATAATGACATACAGTGAATTT), hu ApoB
3G R2 (CAGGTCTCTGCCTTCCTTAGA), huApoB3GFL
(GACATCCCTGGTGGTCCACA-FL) and hu ApoB 3G LC
(LC640-GGTGTCCCAGCAGTGCTTAAA-P...
Retrovirology Research BioMed Central Open Access Suppression of HIV-1 replication by microRNA doc
... (5'-
GUGACAUCCUGCCACCUCACUU(dTdT)-3'), GW182
(5'-UAGCGGACCAGACAUUUCU(dTdT)-3'), XRN1 (5'-
AGA UGA ACU UAC CGU AGA A( dTdT)-3') and CDK9
(5'-CCAAAGCUUCCCCCUAUAATT(dTdT)-3') ... instructions
(Amaxa). siRNA corresponding to DGCR8 (5'-CAUCG-
GACAAGAGUGUGAU(dTdT)-3'), Drosha (5'-CGA-
GUAGGCUUCGUGACUU(dTdT)-3'), RCK/p54 (5'-
GCAGAAAC...