Báo cáo khoa học: "Tezosentan reduces the microvascular filtration coefficient in isolated lungs from rats subjected to cecum ligation and puncture" pot

... = 0 minutes in the CLP+Tezo group.Figure 4 Microvascular filtration coefficient (Kfc) and compliance (CL) after endothelin-1 (ET-1) administration in isolated lungs from healthy ratsMicrovascular ... groups.Figure 6Protein kinase Cα in lungs after endothelin-1 (ET-1) administrationProtein kinase Cα in lungs after endothelin-1 (ET-1) administration. (a) Cytosolic and (b) membrane fractions. ... before and after papaverine administration in isolated lungsPulmonary arterial pressure responses (∆PPA) to endothelin-1 (ET-1) before and after papaverine administration in isolated lungs. ...

Báo cáo khoa học: Assignment of the [4Fe-4S] clusters of Ech hydrogenase from Methanosarcina barkeri to individual subunits via the characterization of site-directed mutants pdf

... correspond-ing to the products of the echABCDEF operon [5]. The EchA and EchB subunits are predicted to beintegral, membrane-spanning proteins, while the otherfour subunits are expected to extrude into ... was used as internal standard. The upper band corresponds to HdrD (43 kDa) and the lower band to EchE (39 kDa). In each lane 6 lg protein from cell extracts of the EchF mutant strains were loaded. ... of binding of the two putative [4Fe-4S] clusters is indica-ted. The M. barkeri strains carrying cysteine to serine mutationsare indicated on the top.Table 1. M. barkeri strains used in this...

Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf

... oxygen) for48 h, and harvested. Total RNA was extracted from each cell line, and then subjected to northern blot analysis. Each lane contains15 lg of total RNA. The bottom panel shows the expression ... HO-1 and HO-2 mRNA. The intensities of the signals in (A)were quantiﬁed, and the intensity representing HO-1 or HO-2 mRNA was normalized with respect to the intensity for the 28S RNA signal. The ... extracts were subjected to western blot analysis (A, C). Other conditions are described in Fig. 1B. The intensities of the signals in (A) and (C) were quantiﬁed, and the intensity representing HO-1...

Báo cáo khoa học: "Taurolidine reduces the tumor stimulating cytokine interleukin-1beta in patients with resectable gastrointestinal cancer: a multicentre prospective randomized trial" doc

... data, and wrote the manuscript. CNG and JS recruited the patients and coor-dinated the study in the other two surgical centres. CM and WW monitored the patients and collected the data in the study ... centrifuged, and supernatants were stored (-25°C). The remaining of the intraperitoneal fluid was col-lected to determine intraabdominal viable tumor cells (in T1 and T4). The volume of the removed ... coordinating center Berlin. JMM contributed to the idea and supported the study. CAJ contributed to the idea, designed the protocol, conceived and coordinated the study, enrolled the patients, gathered...

Tài liệu Báo cáo khoa học: Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR docx

... domainof the MyroBP family possesses different ligand-bind-ing properties, thereby providing a broad binding speci-ﬁcity. In some proteins containing tandem homologousdomains, inter-domain interactions ... proteins containtandem lectin domains as shown in Fig. 6. The tandemdomains present in MyroBP family members may par-ticipate in multivalent sugar binding as observed withother carbohydrate binding ... from B. napus possesses lectin activity, binding to p-amino-phenyl-a-d-mannopyranoside and to some extent to N-acetylglucosamine [10]. Because myrosinase containspotential N-linked sugar-binding...

Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc

... motifArg40Cys84Tyr349Tyr347Lys370Ala36Ala36Ala36Val357Phe518BLaccaseTyrosinaseβ-sheet proteinFig. 1. Overview of the tyrosinase gene and surrounding genes in the genome ofV. spinosum. (A) Showing the tyrosinasegene and those in its immediate vicinity in the V. spinosum genome. ... spinosumtyrosinase. Another motif, which is present not only in tyrosinases, but also in the oxygen transportinghaemocyanin proteins, is the PYWDW (amino acids118–122) and has been hypothesized to be involved ... purely inhibitoryfunction and ⁄ or a role in membrane binding is unclearat present. With regard to V. spinosum pro-tyrosinase,sequence analysis of the C-terminal domain, and indeed of the entire...

Báo cáo khoa học: Mapping of the functional phosphate groups in the catalytic core of deoxyribozyme 10–23 potx

... containing a P-stereorandom single phosphorothioatelinkage in predetermined positions of the catalytic core from P1 to P16,was evaluated under single-turnover conditions in the presence of either3mm ... of the metal-binding site in the catalytic core of deoxyribozyme 10–23 includes the interactions of divalent cations with both the pro-RP and proSPoxygens of P5, and an interaction with the ... coordinated the metal ion, suggestingthat the sulfur atoms in both the proRP and proSPpositions hindered direct contact with metal ions.Interestingly, this PS enzyme regained its activity in the...

Báo cáo khoa học: Distribution of the lipolysis stimulated receptor in adult and embryonic murine tissues and lethality of LSR–/– pdf

... measured in the postprandial stage [3]. The present work was u ndertaken to determine the distribution of LSR mRNA and protein in murine organs, and whether this distribution was c ompatible with the alleged ... put into Trizol (GibcoBRL, Cergy-Pontoise, France)andstoredat)80 °Cpending RNA isolation. Total RNAs were isolated accord-ing to the manufacturer’s instructions. The amount of RNAwas determined ... 2, intron 1, exon 1 and its 5¢-ﬂankingnoncoding sequence, and 2.7 kb of sequence containing the 3 ¢-part of exon 5 and 3¢-ﬂanking intron 5 s equence,respectively. These were i nserted into...

Báo cáo khoa học: Evidence for the presence of ferritin in plant mitochondria pdf

... Triton X -100, which were then subjected to trypsin d igestion. The intensity of the immuno-labeled band was lower in the presence of the detergent,demonstrating that ferritin is localized inside ... ATPaseinhibitor), baﬁlomycin A1(tonoplast ATPase inhibitor) and molybdate (soluble phosphatase inhibitor), but stronglyinhibited by oligomycin (mitochondrial ATPase inhibitor). In addition, ... wasdescribed [14]. Therefore, ferritins can play a critical role in the cellular regulation o f iron storage and homeostasis.Soluble (ferritins) and insoluble (phytosiderin) iron-storage proteins have...

Báo cáo khoa học: Proteolysis of the tumour suppressor hDlg in response to osmotic stress is mediated by caspases and independent of phosphorylation pot

... and triggers itsdissociation from the cytoskeletal protein GKAP,therefore releasing it from the cytoskeleton into the cytoplasm [3].Our aim in this study was to gain a better under-standing ... sorbitol (60 min) and then incubated in sorbitol-free medium (6 h). Detached cells were washedwith NaCl ⁄ Pi and cells remaining attached to the dishwere treated with trypsin and counted using ... 60 min, and then incubated for 3 h in sorbitol-free medium (in the continued absence or presence of the inhibitor). Endogenous levels of hDlg were analysed by immuno-blotting (lower) and the...

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