Báo cáo khoa học: "Tezosentan reduces the microvascular filtration coefficient in isolated lungs from rats subjected to cecum ligation and puncture" pot

... = 0 minutes in the CLP+Tezo group. Figure 4 Microvascular filtration coefficient (Kfc) and compliance (C L ) after endothelin-1 (ET-1) administration in isolated lungs from healthy ratsMicrovascular ... groups. Figure 6 Protein kinase Cα in lungs after endothelin-1 (ET-1) administrationProtein kinase Cα in lungs after endothelin-1 (ET-1) administration. (a) Cytoso...

Ngày tải lên: 12/08/2014, 23:20

... correspond- ing to the products of the echABCDEF operon [5]. The EchA and EchB subunits are predicted to be integral, membrane-spanning proteins, while the other four subunits are expected to extrude into ... was used as internal standard. The upper band corresponds to HdrD (43 kDa) and the lower band to EchE (39 kDa). In each lane 6 lg protein from cell extracts...

Ngày tải lên: 07/03/2014, 21:20

... oxygen) for 48 h, and harvested. Total RNA was extracted from each cell line, and then subjected to northern blot analysis. Each lane contains 15 lg of total RNA. The bottom panel shows the expression ... HO-1 and HO-2 mRNA. The intensities of the signals in (A) were quantified, and the intensity representing HO-1 or HO-2 mRNA was normalized with respect to the in...

Ngày tải lên: 19/02/2014, 06:20

... data, and wrote the manuscript. CNG and JS recruited the patients and coor- dinated the study in the other two surgical centres. CM and WW monitored the patients and collected the data in the study ... centrifuged, and supernatants were stored (- 25°C). The remaining of the intraperitoneal fluid was col- lected to determine intraabdominal viable tumor cells...

Ngày tải lên: 09/08/2014, 04:21

... domain of the MyroBP family possesses different ligand-bind- ing properties, thereby providing a broad binding speci- ficity. In some proteins containing tandem homologous domains, inter-domain interactions ... proteins contain tandem lectin domains as shown in Fig. 6. The tandem domains present in MyroBP family members may par- ticipate in multivalent sugar binding as observed wit...

Ngày tải lên: 18/02/2014, 14:20

... motif Arg40 Cys84 Tyr349 Tyr347 Lys370 Ala36 Ala36 Ala36 Val357 Phe518 B Laccase Tyrosinase β -sheet protein Fig. 1. Overview of the tyrosinase gene and surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinase gene and those in its immediate vicinity in the V. spinosum genome. ... spinosum tyrosinase. Another motif, which is present not only in tyrosinas...

Ngày tải lên: 06/03/2014, 11:20

... containing a P-stereorandom single phosphorothioate linkage in predetermined positions of the catalytic core from P1 to P16, was evaluated under single-turnover conditions in the presence of either 3mm ... of the metal-binding site in the catalytic core of deoxyribozyme 10–23 includes the interactions of divalent cations with both the pro- R P and proS P oxygens of P5,...

Ngày tải lên: 07/03/2014, 10:20

... measured in the postprandial stage [3]. The present work was u ndertaken to determine the distribution of LSR mRNA and protein in murine organs, and whether this distribution was c ompatible with the alleged ... put into Trizol (Gibco BRL, Cergy-Pontoise, France)andstoredat)80 °C pending RNA isolation. Total RNAs were isolated accord- ing to the manufacturer’s instruct...

Ngày tải lên: 16/03/2014, 18:20

... Triton X -100, which were then subjected to trypsin d igestion. The intensity of the immuno- labeled band was lower in the presence of the detergent, demonstrating that ferritin is localized inside ... ATPase inhibitor), bafilomycin A 1 (tonoplast ATPase inhibitor) and molybdate (soluble phosphatase inhibitor), but strongly inhibited by oligomycin (mitochondrial ATPase inhibito...

Ngày tải lên: 16/03/2014, 18:20

... and triggers its dissociation from the cytoskeletal protein GKAP, therefore releasing it from the cytoskeleton into the cytoplasm [3]. Our aim in this study was to gain a better under- standing ... sorbitol (60 min) and then incubated in sorbitol-free medium (6 h). Detached cells were washed with NaCl ⁄ P i and cells remaining attached to the dish were treated with t...

Ngày tải lên: 23/03/2014, 06:20