Báo cáo y học: " Overexpression of cathepsin K during silica-induced lung fibrosis and control by TGF-β" pps

... the Cat K gene were found to be responsible of pycnodysosto- sis in humans [21] and of a similar bone phenotype in mouse [22]. In a murine model of lung fibrosis induced by bleomycin, this cathepsin ... extraction. Hydroxyproline assay Collagen deposition was estimated by measuring hydrox- yproline content in lungs homogenized in PBS. Hydroxy- proline was assessed by hig...

Ngày tải lên: 12/08/2014, 18:21

... differences. Results Characterization of cathepsin K overexpression in the lungs of mice We initially characterized cathepsin K overexpression in the lungs of transgenic mice compared to wild-type mice. Analysis of cathepsin K ... the hypothesis that over- expression of cathepsin K in the lungs of mice is protective in bleomycin-induced pulmonary fibrosis. M...

Ngày tải lên: 12/08/2014, 15:21

... BALF and severity of ALI (lung com- pliance and severity of hypoxemia) [13]. The role of TNFα in pulmonary pathophysiology has been well studied, and includes induction of cellular inflammatory ... >30%; the knockdown of PBEF expression with siRNA significantly inhibited basal and TNFα-stimulated IL-8 secretion by 70% and IL-8 mRNA levels by 74%. Further, the knock...

Ngày tải lên: 11/08/2014, 08:22

... 25:21-25. 21. Sakamoto N, Mukae H, Fujii T, Ishii H, Yoshioka S, Kakugawa T, Sug- iyama K, Mizuta Y, Kadota J, Nakazato M, Kohno S: Differential effects of α- and β-defensin on cytokine production by cul- tured ... 2001, 15:1819-1821. 13. Takemura H, Kaku M, Kohno S, Hirakata Y, Tanaka H, Yoshida R, Tomono K, Koga H, Wada A, Hirayama T, Kamihira S: Evaluation of susceptibility of...

Ngày tải lên: 12/08/2014, 18:20

... by nickel-chelate chromatography to a specific activity of 1.06 UÆmg )1 protein at 2 5 °C and with a yield of 98%. The characterization of the purified enzyme showed that GO is a homotetramer of ... that can be effi ciently purified in a single step by metal-chelate chromatography, producing a stable holoenzyme with a high specific activity and a 98% yield quickly and simply. Its...

Ngày tải lên: 08/03/2014, 16:20

... catalytic activity of b-expansins. These results indicate a common origin of cathepsin B and b-expansins, especially if taken together with their previously known biochemical properties. Keywords: ... and was bound by mAb IG12, but not mAb Bo14 (Fig. 3A,C), indicating truncation of the C-terminus. The different stability of glycosylated and nonglycosylated rPhl p 1 rules out...

Ngày tải lên: 08/03/2014, 22:20

... nuclear lobulation by overexpression of Lyn tyrosine kinase Tomomi Omura, Hiroshi Sakai and Hiroshi Murakami 1 Department of Biotechnology, Faculty of Engineering, Okayama University, Japan Stimulation ... pBluescriptII KS(+) (Strata- gene, La J olla, CA, USA), respectively, and were named pBS-LynN and pBS-LynC. The authenticity of pBS-LynN and pBS-LynC was con®rmed by D...

Ngày tải lên: 17/03/2014, 17:20

... analysis of data. JLC designed flow cytometric assays to characterize recruitment of inflammatory cell population and analysis of data. JMB assisted with design of study, analysis of data, and ... preservation of AM phagocytosis may reflect incomplete blockade of mICAM-1-mediated effects by sICAM-1 . Despite relatively normal phagocytosis by AM, the efficiency of bacte...

Ngày tải lên: 12/08/2014, 13:22

... devel- opment of airway hyperresponsiveness. Furthermore, there was a reduction in the number of inflammatory cells, especially eosinophils, in the lungs and an attenu- ated production of cytokines by lymph ... between OVA/eNOS and OVA/WT mice. C) number of lym- phocytes in BAL fluid. Hardly any lymphocytes could be detected in SAL/WT and SAL/eNOS mice. A markedly increased n...

Ngày tải lên: 12/08/2014, 16:20

... CD45-FITC/IsoClonic Control- PE reagent to check the non-specific binding of the CD34-PE monoclonal antibody. Statistical analysis All data were analyzed by Systat software (Sys- tat Inc) and KaleidaGraph software. ... treatment of obesity by means of the Simeons therapy: a criteria-based meta-analysis. Br J Clin Pharmacol. 1995; 40: 237-243. 27. Sutherland DR, Anderson L,...

Ngày tải lên: 25/10/2012, 10:51