... resuspended in 500 µL of ice-cold PBS and imme-diately analyzed by flow cytometry. For AnnexinV binding analysis 5 x 105 cells were washed once with PBS and resuspended in 500 µL AnnexinV Binding ... B19 infection, direct B19 NS1-induced cytotoxicity may play a role in idiopathic fulminant liver failure. Key words: Parvovirus, B19, Fulminant Liver Failure, Cytotoxicity, Apoptosis INTRODUCTION ... 2. AnnexinV Binding Assay. HepG2 cells were mock, EGFP-, NS1-, PathT-, or PathI-transduced, collected at indicated times post-transduction, and analyzed by flow cytometry for AnnexinV binding....