... [19] catalytic activity. Interestingly,maximal activity of domain 2 was achieved only whendomain 1 was functional, reinforcing, once again, theidea that catalysis at one active site is affected ... isoforms.AbbreviationsAK, arginine kinase; CK, creatine kinase; CyCK, cytoplasmic CK; FlgCK, flagellar CK; kcat, catalytic turnover; MtCK, mitochondrial CK;PCr, phosphocreatine; TSAC, transition state analog ... If each CK domain of theFlgCK has an equal potential for catalytic rateenhancement, then it might be anticipatedthat C fi S mutations in individual domains andTable 1. Kinetic parameters for...