... Biosynthetic pathway leading to clavulanic acid. BLS, beta-lactam synthetase (ORF3); PAH, proclavaminate amidino hydrolase(ORF4); CAS, clavaminate synthase (ORF5); CAD, clavaldehydedehydrogenase ... transfers anacetyl group from N-acetylornithine to glutamate. Hence,once the pathway is initiated from glutamate and acetyl-CoA by acetylglutamate synthase (ARGA), the acetylgroup can be recycled, ... reversible transfer of an acetyl group from N-acetylornithine to glutamate, butnot the formation of N-acetylglutamate from glutamate and acetyl-coenzyme A, nor (detectably) the hydrolysis of N-acetylornithine....