Báo cáo y học: " Human lung cancer cells express functionally active Toll-like receptor 9" potx

... dimeric trans-activator of gene expression or synergistically with other transcription factors such as Sox2 [3]. U ntil recently, Oct4 was thought to be expressed exclusively in embryonic ste m (ES ) cells and primordial ... IgG1,cloneYB5.B8,BDPhar- Mingen Biosciences, UK). The percentage yield and pur- ity of isolated cells was ascertained by flow cytometry: the mononuclear cell fractio...

Ngày tải lên: 11/08/2014, 07:21

... likely type pre- viously reported as SW1 [40]. One hundred and twelve women were found to be infected by a single HPV type and 11 showed co-infection, 9 of which by two types and two by three types. ... Betapapillomavirus and a likely new type, previously reported, from 132 women positive for the virus analysed by Hybrid Capture II assay. These women were infected by a single or multiple HPV...

Ngày tải lên: 11/08/2014, 21:21

... FEV 1 /forced vital capacity (FVC) ≤ 0.70; no history o f asthma, atopy (as assessed by an allergy skin prick test during screening) or any other active lung disease. Patients on home oxygen or with raised carbon ... inflammatory disease of the lung. The nature of the immune reaction in COPD raises the possibility that IL-17 and related cytokines may contribute to this disorder. This stu...

Ngày tải lên: 12/08/2014, 13:22

... P4H isoenzyme gene expression in articular chondrocytes aggregatesCollagen P4H isoenzyme gene expression in articular chondrocytes aggregates. Prolyl 4-hydroxylase (P4H)α isoenzyme gene expression ... 25:402-408. 52. Takahashi Y, Takahashi S, Shiga Y, Yoshimi T, Miura T: Hypoxic induction of prolyl 4-hydroxylase alpha (I) in cultured cells. J Biol Chem 2000, 275:14139-14146. 53. Chun Y-...

Ngày tải lên: 09/08/2014, 10:20

... played by Th17 cells in the pathogenesis of human autoimmune disorders, although very probable, is not yet proven. More importantly, the respective roles of Th17 and Th1 cells in inflammatory ... although IL-12 polarized cells (prototypic Th1 cells) expressed genes associated with cytotoxicity, such as those encoding IFN-γ, Fas ligand and granzymes, IL-23 polarized cells expressed...

Ngày tải lên: 09/08/2014, 10:23

... donkey anti-goat IgG biotin- conjugated secondary antibody (Santa Cruz Biotechnology) for collagen type I and collagen type II and donkey anti-rabbit IgG biotin-conjugated secondary antibody for ... heterogeneity in the cell popula- tion but was an epitope expressed by all cells, but only during part of the cell cycle. It is thus possible that IPFP-derived cells were a homogenously 3G5-pos...

Ngày tải lên: 09/08/2014, 10:23

... (both for naive and memory T cells) , cytotoxic T-lymphocyte formation, IFN-γ production by Th1 cells, and interleukin-4 pro- duction by Th2 cells. [39,40]. The ability to decrease IFN-γ production, ... of mesenchymal progenitors. Stem Cells 2005, 23:220-229. 26. Yen BL, Huang HI, Chien CC, Jui HY, Ko BS, Yao M, Shun CT, Yen ML, Lee MC, Chen YC: Isolation of multipotent cells from hum...

Ngày tải lên: 09/08/2014, 10:23

... evaluation. Functional analysis of hES-DCs by Mixed Leukocyte Reaction (MLR) assay and antigen uptake assay The T cell stimulatory capacity of DCs derived from hES cells CD34+ progenitor cells was assessed by co-incubat- ing ... 4 µg/ml polybrene was used. Infected cells were visualized by fluorescence microscopy to identify GFP expressing cells. Infected culture supernatants were als...

Ngày tải lên: 10/08/2014, 05:20

... putative immediate-early locus of human herpesvirus 6. J Virol 1991, 65:5381-5390. 30. Takemoto M, Koike M, Mori Y, Yonemoto S, Sasamoto Y, Kondo K, Uchiyama Y, Yamanishi K: Human herpesvirus 6 ... on heterologous expression in mammalian cells. Nucleic Acids Res 1991, 19:3979-3986. 25. Somboonthum P, Yoshii H, Okamoto S, Koike M, Gomi Y, Uchiyama Y, Takahashi M, Yamanishi K, Mori...

Ngày tải lên: 11/08/2014, 21:21

... biopsy was performed when the results of pleural fluid analysis were suggestive of malignancy. Flow Cytometry The expression markers on T cells from MPE and blood were determined by flow cytometry ... Respiratory Diseases, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, China. 2 Institute of Resp...

Ngày tải lên: 12/08/2014, 13:22