Báo cáo y học: "Changes in the expression of NO synthase isoforms after ozone: the effects of allergen exposure" doc

... 11:1709-1715.Western blot analysis of the expression of nNOS, eNOS, and iNOS in the lung tissueFigure 2Western blot analysis of the expression of nNOS, eNOS, and iNOS in the lung tissue. Mice were ... are involved in the physiological regulation of air-way function, and an inducible form of the enzyme(iNOS) is involved in inflammatory disease of the airwaysand in host defense against infection ... roles of neuronal NOS (nNOS),endothelial NOS (eNOS), and iNOS in the murine model of asthma with ozone exposure are uncertain.This study investigated the roles of the individual NOSisoforms...

Báo cáo y học: "Changes in gene expression during the development of mammary tumors in MMTV-Wnt-1 transgenic mice" ppt

... cells in the vicinity of the mammary glands. In these analyses, we foundthat the progression of normal virgin mammary glands to hyperplastic tissues and to mammarytumors is accompanied by differences ... the initiating pathway determines a distinctive expression phenotype in tumors. In addition, using proteinsas markers of cell phenotypes, we showed that initiatingoncogenes determine the developmental ... their use in defining expression patterns in tumor evolution and in correlating genotypes withphenotypes has been limited because of the poor availability of tissues at different stages in cancer...

Báo cáo y học: " Changes in microRNA expression profiles in HIV-1-transfected human cells" pptx

... extended using Klenow (3'→5' exo-) in the presence of biotinylated-dATP. Streptavidin-Alexa fluor 555 is then used to bind the biotin group permitting the fluorescent detection of hybridized ... each other. In this comparison, most of the dots line up together at the center line supporting that the miRNA expression pat-terns of the two samples (1 and 2) are highly similar. By contrast, ... purposes of verifying internal reproducibility of hybridization, each probe on the microarray slide was printed 4 times (spots 1, 2, 3 and 4 labeled at top of each column). The identity of individual...

Báo cáo y học: "Changes in protein expression during honey bee larval development" doc

... substantial synthesis of hexamerins and lipopro-teins in the fat body, followed by their appearance in the Table 4Enrichment analysis of protein classes following hierarchical clusteringNode number ... R156.8Genome Biology 2008, 9:R156Average-linkage clustering of proteins quantified in the honey bee larvaeFigure 4 Average-linkage clustering of proteins quantified in the honey bee larvae. Proteins ... proteinsequences of: Honey Bee Official Gene Set 1 [42], commonexogenous contaminants (human and sheep keratins) andadditives (porcine trypsin, lysyl endopeptidase C), the poly-protein of the deformed wing...

Báo cáo Y học: Changes in ultrastructure and the occurrence of permeability transition in mitochondria during rat liver regeneration ppt

... membrane integrity at24 h after PH. The increase in the number of lysosomes,even if at a low extent, the presence of autophagosomes and the reduction in the number of mitochondria that weobserve in ... (20 lgofprotein)orcytosolic (90 lg of protein) preparations were loaded onto anSDS/polyacrylamide gel. Gels were then incubated in amedium containing tetrametylbenzidine in 10% isopropanoland ... also determined in the mitochondrial andcytosolic fractions, and in the whole liver homogenate. The enzyme activity of mitochondrial AAT in the cytosol wasdetermined as described by Greco et...

Báo cáo y học: "Changes in Two Point Discrimination and the law of mobility in Diabetes Mellitus patients" pps

... recorded in the datasheet.If the subject had callosity in any of the foot area, the TPDmeasurement was taken in the adjacent area to the callos-ity but within the same area of the foot. The subject's ... sensory loss in DM subjects.This paper presents the first systematic study of the law of mobility to assess the sensibility of diabetic subjects, com-paring the law of mobility of TPD in upper ... had the TPD values merely increased(from normal value) uniformly over all the areas in the left leg, still satisfying the law of mobility, typical sign of micro vascular dysfunction is shown in...

Báo cáo y học: "Changes in the mechanical properties of the respiratory system during the development of interstitial lung edema" ppt

... slightlyincrease in a similar way in the control and in the treatedgroup. An increase in Raw can be associated to a reduction of airway calibre but, since in interstitial lung edema wedid not find ... declare that they have no competing interests.Authors' contributionsRLD Contributed in the design of the study, in writing the manuscript, in the design and development of the experi-mental ... 2), the use of a higher amplitude at low frequency did not significantlyaffect the assessment of Zrs in our experimental condi-tions.Non-linearity of the respiratory system may also affect the measurements,...

Báo cáo y học: " Changes in the accessibility of the HIV-1 Integrase C-terminus in the presence of cellular proteins" doc

... added a biotinylable tag at the C-terminus of IN (IN- BAD) and showed that the pro-tein remains fully active in the context of a lentiviralvector. The kinetics of viral DNA synthesis and integra-tion ... C-terminus. The IntegraseBinding Domain (IBD) of LEDGF/p75 interacts with the IN- CCD, but no interaction with the IN- CTD has beendocumented [11,12,27]. It is therefore likely that the masking ... that the biotinylated tag at the C-terminus of the IN prot ein,which can be detected in virions, becomes inaccessible forstreptavidin binding after entry into the cell.Efficient co-immunoprecipitation...

Báo cáo y học: " Changes in the central component of the hypothalamus-pituitary-thyroid axis in a rabbit model of prolonged critical illness" ppsx

... lower TRH expression in the face of normal or low thyroidhormone levels could be an increased activity of the availablethyroid hormone by increasing the expression of the nuclearthyroid hormone ... thyrotoxicosis in the hypothala-mus. Increased hypothalamic T3 availability could indeedexplain feedback inhibition-induced suppression of the TRHgene in the context of the low T3 syndrome. ... albumin; CV: coefficient of variation; D1: type 1 iodothyronine deiodinase; D2: type 2 iodothyronine deiodinase; D3: type 3 iodothyronine deiodinase; HPRT: hypoxanthine guanine phosphoribosyl...

Báo cáo y học: "Changes in serum creatinine in the first 24 hours after cardiac arrest indicate prognosis: an observational cohort study" ppt

... prog-nosis depends more on the degree of hypoxic brain damagethan on the underlying disease [10]. The extent of hypoxic brain damage can be estimated bymeasurement of serum levels of the enzyme ... being a majorcontributor to the development of AKI in critically ill patients[9]. However, in the majority of patients it remains unknownwhether AKI is caused by systemic versus renal hypoper-fusion, ... assessed at the time of ICU dis-charge according to the Pittsburgh cerebral performance cat-egory (CPC) [15]. The classifying physician was blinded to the intention of the study. CPC 1 and...

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