Báo cáo y học: "TGF-β1 and serum both stimulate contraction but differentially affect apoptosis in 3D collagen gels" ppt

... citation purposes) Respiratory Research Open Access Research TGF-β1 and serum both stimulate contraction but differentially affect apoptosis in 3D collagen gels Tetsu Kobayashi 1 , Xiangde Liu 1 , ... family, and inhibition of both cIAP-1 and XIAP, two inhibitors of the caspase cascade. Serum was associated with an increase in cIAP-1 and Bcl-2, anti-apoptot...

Ngày tải lên: 12/08/2014, 18:20

... massively parallel sequencing in cases where routine phenotype testing could not be employed. While the Trofile phenotyping assay is currently the only diagnostic tool for enrollment of patients in ... amplification and pooling, sequence alignments and interpretations), ii) the availability of the final product in a reproducible and standardized way, and iii) the cost per sample...

Ngày tải lên: 10/08/2014, 05:21

... kidney, EMT can be induced by TGFβ 1 , leading to increased collagen deposi- tion and disruption of the epithelial integrity [17]. TGFβ 1 is known to be expressed by a variety of inflammatory and structural ... have increased immunoreactivity for MMP-9 in their airway epithelium and submucosa [53,54]. Overexpression of MMP-proteins could further contribute to airway remod- eling in...

Ngày tải lên: 12/08/2014, 14:20

... muscle hypertrophy and hyperplasia. Moreover, our finding that TGF-β1 enhances serum- induced ASMC proliferation may occur in asth- matic airways where there is inflammation leading to increase in ... and valley formation" and by immunocytochemistry staining for α-smooth muscle actin and smooth muscle-specific myosin heavy chain (SM1 and SM2). For the experiments, the ASMC...

Ngày tải lên: 12/08/2014, 16:20

... cav-1α and cav-2 are closely associ- ated in ciliated and in basal cells, indicating that both proteins are involved in the formation of caveolae. Inter- estingly, we found considerably higher ... ascending aorta with a rinsing solution containing heparin (2 ml/l; 10,000 U; Ratiopharm, Ulm, Germany), polyvinylpyrro- lidone (25 g/l, MW 40.000; Roth, Karlsruhe, Germany) and procai...

Ngày tải lên: 12/08/2014, 16:20

... Italy. 4 Department of Internal Medicine, Aging and Nephrology, Infectious Diseases Section, University of Bologna, Italy. 5 Department of Haematology, Oncology and Clinical Pathology, University ... X4 and R5 laboratory strains represented by HIV-1 IIIb and HIV-1 ada respectively. Total DNA, collected and purified at days 3 and 7 post- infection, was analyzed by PCR, and both...

Ngày tải lên: 13/08/2014, 01:20

... binding was assessed by flow cytometry following addition of a secondary FITC-conjugated anti rabbit IgG antibody. Similar results were obtained in binding assays performed using all cell lines ... PBS-0.1% Tween 20 and revealed by chemiluminescence (ECL+, Amersham). Binding and binding interference assays Binding assays were performed as previously described [31]. Briefly, 5 × 10 5...

Ngày tải lên: 13/08/2014, 13:20

... established in the criti- cally ill characterized by tissue ischemia-reperfusion injury and by an intense systemic inflammatory response such as during sepsis and acute respiratory distress syn- drome ... production, protein oxidation, and/ or enzyme activity, is time consuming and impractical in a clinical setting. More importantly, this type of analysis may miss other contributin...

Ngày tải lên: 13/08/2014, 23:21

... findings in earlier surveys, this survey indicates a trend towards increased involvement of academic departments of child and adolescent psychiatry in training medical students. Introduction In ... adult psychiatry nationally and also internation- ally will contribute to standardizing goals and teach- ing methods and hence improving education for medical students. In contrast...

Ngày tải lên: 25/10/2012, 10:06

... are involved in hemagglutination, lymphocyte transformation, inactivation of certain types of tumor cells and precipitation of certain polysaccharides and glycoproteins [1,2]. Plant lectins isolated ... cm) using lysozyme, trypsin inhibitor, a-amylase, BSA and b-amylase as reference proteins. Molecular mass determination: SDS/PAGE. SDS/PAGE was conducted on a 10% acrylamide gel accord...

Ngày tải lên: 18/03/2014, 01:20