Báo cáo y học: "Decreased catalytic function with altered sumoylation of DNA topoisomerase I in the nuclei of scleroderma fibroblasts" ppsx

... SUMO1 inhibition in the fibroblasts. According to the intensity of the bands of remaining supercoiled DNA in serial dilutions in the assays of three fibroblast strains, these changes are significant. ... and then stained with ethidium bromide. The catalytic activity of topo I was determined by measuring the intensity of the super- coiled DNA bands after...

Ngày tải lên: 12/08/2014, 17:22

... machinery including the core histone proteins resulting in their in re-association with the DNA, thus presenting a transcriptionally closed con- formation [1,16]. HDAC-2 function is impaired by ... which is relatively insensitive to glucocorticoids. This is prevented by aboli- tion of PI3K signalling and glucocorticoid treatment. Therefore, blockade of PI3K signalling in co...

Ngày tải lên: 11/08/2014, 03:20

... machinery including the core histone proteins resulting in their in re-association with the DNA, thus presenting a transcriptionally closed con- formation [1,16]. HDAC-2 function is impaired by ... PI3K signalling in combination in combination with glucocorticoid treatment may provide a strategy to overcome an oxidant-induced reduction in responsiveness to the anti-infl...

Ngày tải lên: 11/08/2014, 06:22

... mass distribution LS/UV/RI 3-detector analysis. Binding kinetics and affinity studies The binding affinities an d kinetics of BLyS (average values were determined from three distinct lots of pro- tein), ... more-detailed descriptions of the generation, purifi- cation, and characterization of the recombinant hetero- trimers, and of the binding affinity and biologic activity a...

Ngày tải lên: 12/08/2014, 12:20

... (P aw ) is applied during inspiration and expiration, thus avoiding end- inspiratory overdistension, while maintaining end-expiratory alveolar recruitment. By optimizing alveolar recruitment, ... 342:1301- 1308. Competing interests JMS and SM work in the intensive care unit of the Mount Sinai Hospital in Toronto, Canada, which has received the “3100B high frequency oscillatory ven...

Ngày tải lên: 12/08/2014, 18:21

... homology, or being structurally similar but enzymatically inactive (DPP6, DPP10). In some instances, the DPPIV activity is clearly intrinsic whereas in others the nature of the DPPIV activity remains ... preference by proteolytic modification; black arrows indicate stimulation of indicated mediator or enzymatic activity; dashed lines indicate termination of indicated mediator...

Ngày tải lên: 09/08/2014, 07:20

... cartilage. TGFβ- induced synovial fibrosis was blocked by locally inhibiting TGFβ signaling in the synovial lining by simultaneously transfecting it with an adenovirus overexpressing Smad7. Introduction Osteoarthritis ... content in cartilage, even in experimental OA. In addition, co-transfecting the synovial lin- ing with Ad-Smad7 can block the fibrosis that is induced by T...

Ngày tải lên: 09/08/2014, 07:20

... radial head. The reduction was achieved in supination and immobilisation of the elbow in flexion and supination gave a favourable final outcome. In the presence of major distracting injuries like ... contributions UR was involved in collecting patient details, reviewing the literature and drafted the manuscript as the main author. RSUY was involved in reviewing the...

Ngày tải lên: 11/08/2014, 10:22

... evaluation. However, the timing of neutrophil inhibition has to be critically considered since inhibition of neutrophil activa- tion might impair anti bacterial phagocytic effects of neu- trophils which are ... data/sample collection Begin Bleeding MAP 35 ± 5 mmHg Reperfusion End of extracorporeal therapy Begin experiment 15 min Begin of extracorporeal therapy Therapy 0min 1...

Ngày tải lên: 11/08/2014, 03:20