Báo cáo y học: " Investigating a pathogenic role for TXNDC5 in rheumatoid arthritis" ppsx

... primer for TXNDC5, 5’ -GCCTCCA CTGTGCTCACTGA-3’;forwardprimerforhumanb- actin, 5’-TGGCACCCAGCACAATGAA-3’;andreverse primer for human b-actin, 5’-CTAAGTCATAGTCCGCC- TAGAAGCA-3’. Primer efficiency was ... using a plate reader (Synergy HT, Bio-Tek, Winooski, VT, USA). We repeated the ELISA three times and obtained the similar results. Sandwich ELISA has low inter-assay and intra-assay var...

Ngày tải lên: 12/08/2014, 17:22

... gel zymography assay, which partly prove that CD147 upregulation on macrophages may increase MMP production through both autocrine and paracrine stimulation and that macrophages may act as an amplifier ... antibody and AP9 Based on the reports that CD147 is a high affinity receptor for CyPA and responsible for a cyclophilin signaling cascade that culminates in extracellular signal-...

Ngày tải lên: 09/08/2014, 06:23

... HLA shared-epitope genotypes for rheumatoid arthritis. Am J Hum Genet 1996, 58:371-383. 23. Kochi Y, Yamada R, Kobayashi K, Takahashi A, Suzuki A, Sekine A, Mabuchi A, Akiyama F, Tsunoda T, Nakamura ... USA) and the help of technical and clinical collaborators from the rheumatoid arthritis international working group, for clinical data management and laboratory typings. The a...

Ngày tải lên: 09/08/2014, 10:23

... conventional grey- scale (B-mode) ultrasonography (US) in the evaluation of RA synovial inflammation and joint damage indicate that clinical joint examination and CR are comparatively insen- sitive ... damage to joints in rheumatoid arthritis (RA) is essential in both routine clinical management and clinical trials. But does conventional radiography (CR) remain the gold-standard metho...

Ngày tải lên: 09/08/2014, 01:23

... colleagues [15] noted a higher average urinary nitrate/creatinine ratio in patients with RA than in controls after only an overnight fast. Our analyses showed that urinary NOx and NOx/creatinine ... in any medium, provided the original work is properly cited. Abstract Nitric oxide (NO) may play important roles in rheumatoid arthritis (RA). RA is an inflammatory disease involving j...

Ngày tải lên: 09/08/2014, 08:22

... membrane analysis of synovial fibroblast supernatantsProteomic membrane analysis of synovial fibroblast supernatants. (a) Comprehensive protein membrane array map. The human protein array simulta- neously ... Kojima F, Naraba H, Miyamoto S, Beppu M, Aoki H, Kawai S: Membrane-associated prostaglandin E synthase-1 is upregu- lated by proinflammatory cytokines in chondrocytes from patients wit...

Ngày tải lên: 09/08/2014, 10:22

... Takizawa Y, Suzuki A, Sawada T, Ohsaka M, Inoue T, Yamada R, Yamamoto K: Citrullinated fibrinogen detected as a soluble cit- rullinated autoantigen in rheumatoid arthritis synovial fluids. Ann Rheum ... (Dako, Carpinteria, CA, USA). For fibrinogen and IgG staining, pre- treatment of proteinase K (Dako, Carpinteria, CA, USA) was used before the primary antibody incubation. Slides pos...

Ngày tải lên: 09/08/2014, 10:23

... Gerards AH, Landewe RB, Prins AP, Bruyn GA, Goei The HS, Laan RF, Dijkmans BA: Cyclosporin A monotherapy versus cyclosporin A and methotrexate combination therapy in patients with early rheumatoid ... hemoglobin, alkaline phosphatase, body mass index and the DAS28-3, the HAQ score and the Larsen score at baseline. Bivariate analysis was performed with all factors and covari- ates, and...

Ngày tải lên: 09/08/2014, 13:22

... second- ary staining of biotinylated NGF was added and cells were incubated for 30 minutes at 4°C. After three washes (300 g/ three minutes) with PBS/BSA, cells were ready for FACS analysis. Phenotypic ... NGF is also involved in humoral immune responses by acting as an autocrine survival factor maintaining the viability of memory B-cells and macrophages [27]. NGF and its receptors have...

Ngày tải lên: 09/08/2014, 14:21

... major cause of morbidity and mortality in patients with chronic kidney disease (CKD) patients. Serum albumin, a negative acute-phase reactant and marker for underlying inflammation and/or malnutrition, ... CVD in early CKD stages; b) hypoalbu- minaemia may be used to identify the population at higher risk for CVD. Key words: Hypoalbuminaemia, cardiovascular disease, chronic kidne...

Ngày tải lên: 03/11/2012, 11:52