... Nakamura K, Tokunaga Y, Hanada S, Kumemura H, Maeyama M, Harada M, Ogata H, YanoH, Kojiro M, Ueno T, Yoshimura A, Sata M: Spreds, inhibitors of the Ras/ERK signal transduction, are dysregulated ... used for cluster analysis andgeneration of a heat map.Quantitative real-time PCR The same RNA used for the microarray experiments was alsoused for the quantitative real-time PCR confirmation ... normalize the expression data (fluorescence inten-sities) for the mean intensity of all 12 arrays. Genes expressedin all 12 arrays were selected for analyses. Normalized datawere analyzed...