... 35 cycles consisting of 94°C for min., 94°C for 20s, 55°C for 20s, and 72°C for 1 min. with a final extension of 5 min. at 72°C and 4°C.Publish with BioMed Central and every scientist can read ... 58°C for 30s, and 72°C for 40s, and an additional 20 cycles of 94°C for 30s, 60°C for 30s, and 72°C for 45s with an extension of 7 min at 72°C and incubation at 4°C. Genotypes of HBV for each samplewere ... and PCR buffer, as in the first reaction. Thesewere amplified for3 5 cycles with the following parame-ters: preheating at 94°C for 3 min, 15 cycles of amplifica-tion at 94°C for 20s, 58°C for...