... min, and fivecycles of 94°C for 30 sec, 59°C for 2 min and 72°C for 30sec, and then 35 cycles of 94°C for 30 sec, 61°C for 30 sec and 72°C for 30 sec, followed by a final extension step of 72°C ... and random hexamerspdN6.Five μl of the template cDNA were added to 45 μl of thePCR reaction mixture containing 0.2 μM primers (seeAdditional file 4), 200 μM each of dATP, dCTP, dGTP and dTTP, ... Control of Foot -and- Mouth Disease (EUFMD). InternationalControl of Foot -and- Mouth Disease: Tools, trends and perspectives inCyprus, 16–20 October 2006 .8. European Commission for the Control of...