Báo cáo sinh học: "Mapping sequences by parts" potx

... evolutions " ;by blocks" of certain elements of sequences: minisatellites [5] or swaps in proteins sequences [6]. In the latter, the method is based on selec- tion of common segments by local ... studies of sequences (two pairs of proteins, a pair of DNA sequences of transposon elements and a pair of sequences of genes of microbial genomes) in which the homologies cannot...

Ngày tải lên: 12/08/2014, 17:20

... recovery rate and depletion efficiency up to 5.7 Log by enriching cells by means of a flow-through system [27]. A similar recovery rate was obtained by Zigeuner et al., who compared in spiking experiments ... were harvested by incu- bating with PBS containing 5 mM EDTA for 10 min at 37°C. After washing with PBS containing 2 mM EDTA, cells were counted, and their viability was assessed...

Ngày tải lên: 18/06/2014, 19:20

... full-length rRNA sequences for phylo- genetic analyses. Larger fragments (over 1,000 nucleo- tides) contain more phylogenetic signal and allow us to compare and classify environmental sequences relative ... limited number of full-length sequences in our current eukaryotic SSU rRNA database (several orders of magnitude fewer than the available bacterial and archaeal rRNA sequences)...

Ngày tải lên: 06/08/2014, 19:21

... nucleosome exclusion by polyA and related sequences is the dominant feature in in vitro nucleosome-reconstitution assays. Second, the lack of support for ‘pronucleosomal’ sequences as major ... example, selections for tight-binding sequences provided the chromatin community with the best-defined ‘pronucleosomal’ sequence, the ‘Widom601’ sequence (identified by Jon Widom and co...

Ngày tải lên: 06/08/2014, 19:21

... as calculated by Milan et al (1995). The order of the markers presented in the multipoint linkage map (fig 2) is in agreement with the map published by Rohrer et al ... this trait is influenced by a major gene, called the RN gene, with two alleles, the dominant RN- allele and the recessive rn + allele. Studies by Feddern (1994) reveal...

Ngày tải lên: 09/08/2014, 18:22

... research was supported by Award No 92-01732 of the National Research Initiative Competitive Grants Program of the US Department of Agriculture and by the Holstein Association ... and for reductions in the number of equations by using a reduced animal model (RAM) (Cantet and Smith, 1991; Goddard, 1992), by including QTL gene effects only fo...

Ngày tải lên: 09/08/2014, 18:22

... (CI) for the QTL position calculated by the LOD drop- off method of Lander and Botstein (1989). The limits of this CI were found by determining the map position at either ... are presented in table IV. Again, data sets were analyzed by first treating sires as unrelated and then by utilizing relationships among sires with linkage phases...

Ngày tải lên: 09/08/2014, 18:22

... replaced by a pure D simulans. Interestingly, the tolerance to aliphatic acids returned to the low level typical of D simulans, in spite of the probable selection imposed by ... females to be more tolerant than males, and the small sex by genotype interaction is difficult to explain by specific genes on the X-chromosome. We may conclude...

Ngày tải lên: 09/08/2014, 18:22

... supported by Award No 92-01732 of the National Research Initiative Competitive Grants Program of the US Department of Agriculture, by the Holstein Association USA, and by ABS ... QTL positions, as described by Grignola et al (1996a) for a single QTL model. The log residual likelihood for the animal model was obtained by adding correction...

Ngày tải lên: 09/08/2014, 18:22

... score, every possi- ble l - mer in each of the t sequences must be examined. This is done so by multiplying the respective Q i, j /Q 0, j dic- tated by the nucleotides and their respective positions within ... l-mers repeatedly in an effort to converge to a global optimum by deterministically and systematically searching the solu- tion space modeled by our dynamical system and impr...

Ngày tải lên: 12/08/2014, 17:20