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Báo cáo sinh học: "Mapping sequences by parts" potx

Báo cáo sinh học:

Báo cáo sinh học: "Mapping sequences by parts" potx

... evolutions " ;by blocks" of certain elements of sequences: minisatellites [5] or swaps in proteins sequences [6]. In the latter, the method is based on selec-tion of common segments by local ... studies of sequences (two pairs of proteins, a pair of DNA sequences of transposon elements and a pair of sequences of genesof microbial genomes) in which the homologies cannotbe reported by a classic ... the approach to retrieve seg-ments of sequences related by evolution, we apply a givennumber of evolutionary events (mutations and shuffles)to random sequences and we measure the intersectionbetween...
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Báo cáo sinh học: " Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" pptx

... recovery rate and depletion efficiency up to 5.7 Log by enriching cells by means of a flow-through system[27]. A similar recovery rate was obtained by Zigeuneret al., who compared in spiking experiments ... were harvested by incu-bating with PBS containing 5 mM EDTA for 10 min at37°C. After washing with PBS containing 2 mM EDTA,cells were counted, and their viability was assessed by trypan blue ... subse-quently analysed by flow cytometry. The assay wasrepeated 3 times to validate the reproducibility of themethod.Patients’ SpecimensSamples CollectionThe investigation was approved by the Ethics...
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Báo cáo sinh học: "Mapping the protistan ‘rare biosphere’" docx

... full-length rRNA sequences for phylo-genetic analyses. Larger fragments (over 1,000 nucleo-tides) contain more phylogenetic signal and allow us to compare and classify environmental sequences relative ... limited number of full-length sequences in our current eukaryotic SSU rRNA database (several orders of magnitude fewer than the available bacterial and archaeal rRNA sequences) complicates the ... cultivated protists or identification of eukaryotic rRNA sequences from directly sequenced metagenomic studies could provide more reference sequences. Further challenges in assessing eukaryotic...
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Báo cáo sinh học: " Chromatin ‘programming’ by sequence - is there more to the nucleosome code than %GC" pot

... nucleosome exclusion by polyA and related sequences is the dominant feature in in vitro nucleosome-reconstitution assays. Second, the lack of support for ‘pronucleosomal’ sequences as major ... example, selections for tight-binding sequences provided the chromatin community with the best-defined ‘pronucleosomal’ sequence, the ‘Widom601’ sequence (identified by Jon Widom and colleagues), ... Initial studies focused on pronucleosomal sequences with a 10-nucleotide periodicity of AT, AA, or TT dinucleotides. Two early studies agreed that such sequences were enriched at the +1 nucleosome...
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Báo cáo sinh học: "Mapping the porcine RN gene to chromosome 15" pdf

... as calculated by Milan et al (1995).The order of the markers presented in the multipoint linkage map (fig 2) isin agreement with the map published by Rohrer et al ... this traitis influenced by a major gene, called the RN gene, with two alleles, the dominantRN- allele and the recessive rn+ allele.Studies by Feddern (1994) revealed ... genotypes and excluded fromthe study. The thresholds 15 and 80 J1.mol/g were chosen by visual inspection ofthe glycogen distribution. Of course these thresholds are somewhat...
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Báo cáo sinh học: "Mapping quantitative trait loci in outcross populations via residual maximum likelihood. I. Methodology" pps

... research was supported by Award No 92-01732 of the National Research InitiativeCompetitive Grants Program of the US Department of Agriculture and by the HolsteinAssociation ... and for reductions in thenumber of equations by using a reduced animal model (RAM) (Cantet and Smith,1991; Goddard, 1992), by including QTL gene effects only for genotyped ... the QTL alleles in i are identified by the alleles at the left marker (1,2)whereas if the QTL alleles in i are identified by parental origin,Note that summing the...
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Báo cáo sinh học: " Mapping quantitative trait loci in outcross populations via residual" ppt

... (CI) for the QTL position calculated by the LOD drop-off method of Lander and Botstein (1989). The limits of this CI were found by determining the map position at either ... are presented in table IV. Again,data sets were analyzed by first treating sires as unrelated and then by utilizingrelationships among sires with linkage phases of ... research was supported by Award No 92-01732 of the National Research InitiativeCompetitive Grants Program of the US Department of Agriculture and by the HolsteinAssociation...
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Báo cáo sinh học: "Genetic analysis by interspecific crosses of the tolerance of Drosophila sechellia to major aliphatic acids of its host plant" ppt

... replaced by a pure D simulans. Interestingly, the toleranceto aliphatic acids returned to the low level typical of D simulans, in spite of theprobable selection imposed by ... females to be more tolerant than males, and the small sex by genotypeinteraction is difficult to explain by specific genes on the X-chromosome. We mayconclude that, ... and D sechellia were established by mixing isofemalelines collected in the Seychelles in 1985. Interspecific hybrids were produced by crossing females of D simulans with...
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Báo cáo sinh học: "Mapping linked quantitative trait loci via residual maximum likelihood" docx

... supported by Award No 92-01732 of the National Research InitiativeCompetitive Grants Program of the US Department of Agriculture, by the HolsteinAssociation USA, and by ABS ... QTL positions, asdescribed by Grignola et al (1996a) for a single QTL model. The log residuallikelihood for the animal model was obtained by adding correction terms ... model postulated by the REML analysis, the vector of QTL alleliceffects is random with a prior normal distribution. The REML analysis builds onearlier work by Fernando and...
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Báo cáo sinh học: "Refining motifs by improving information content scores using neighborhood profile searc" ppt

... score, every possi-ble l - mer in each of the t sequences must be examined.This is done so by multiplying the respective Qi, j/Q0, j dic-tated by the nucleotides and their respective positionswithin ... l-mersrepeatedly in an effort to converge to a global optimum by deterministically and systematically searching the solu-tion space modeled by our dynamical system and improv-ing the quality of the ... results of the Exit-point methodologyon real biological sequences. We have chosen l = 20 and d= 2. 't' indicates the number of sequences in the real data.For the biological samples...
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