Báo cáo y học: "Endogenous laminin is required for human airway smooth muscle cell maturation" pot

... prolifera- tion of human airway smooth muscle. Am J Physiol Lung Cell Mol Physiol 2006. 26. Frid MG, Moiseeva EP, Stenmark KR: Multiple phenotypically dis- tinct smooth muscle cell populations exist in the ... E: Distribution of phenotypically disparate myocyte subpopula- tions in airway smooth muscle. Can J Physiol Pharmacol 2005, 83:104-116. 34. Halayko AJ, Rector E, Ste...

Ngày tải lên: 12/08/2014, 16:20

... of RV and human airway smooth muscle cells (HASM). We hypothesised that rhinovirus induction of inflammatory cytokine release from airway smooth muscle is augmented and differentially regulated ... HASM cells, and stored at -80°C, for analysis by ELISA and RV titration assays. HASM cell viability was deter- mined using manual cell counting and trypan blue exclu- sion ass...

Ngày tải lên: 12/08/2014, 16:20

... contributions WIC was responsible for carrying out the experiments, for data analysis, and for drafted this manuscript; KYK was responsible for the analysis and design for the histologic study; OS oversaw the ... slide. Injurious variables included 1) airway epithelial shedding, 2) airway epithe- lial edema, 3) increased cellularity in the airway and par- enchymal tissues, 4)...

Ngày tải lên: 12/08/2014, 13:22

... extracellular purines and pyrimidines on human airway smooth muscle cells. J Pharmacol Exp Ther 2005, 315:941-948. 28. Tao F, Chaudry S, Tolloczko B, Martin JG, Kelly SM: Modulation of smooth muscle ... novel roles in human airway smooth muscle cell contraction and migration. Am J Physiol Cell Physiol 2006, 291(5):C957-65. 26. Randell SH, Walstad L, Schwab UE, Grubb BR...

Ngày tải lên: 12/08/2014, 14:20

... ~5 × 10 4 cells/ml chroni- cally HIV-1 infected CEM cells. Virus production was monitored visually for cytopathic effects and by CAp24 ELISA. AdOx experiments were performed 8 days post co- culture. Treatment ... modify arginine residues by adding one or two methyl groups resulting in three distinct forms of methylated arginine residues in eukaryotes, ω-N G -monomethylarginine (MMA), asymm...

Ngày tải lên: 13/08/2014, 09:20

... 4-8- cell stage. Luciferase activity was measured as described [29]. Tissue culture, immunocytochemistry and subcellular fractionation Rat-1 fibroblasts, human embryonic kidney (HEK) 293 cells and ... hypotonic lysis buffer. Immunoprecipitation and western blotting Immunoprecipitation and western analysis were carried out with cell and embryo lysates as described [14]. To prepare embry...

Ngày tải lên: 06/08/2014, 18:21

... and WWK performed Immunoblotting assay. LMC and WKC performed cell motility assay. JMH and FJT performed integrity of the data and the accuracy of the data analysis. CJL performed study concept ... Subsequently, we observed the ability of motil ity in LoVo cells by migration assay. Because DNA synth- esis was completely inhibited by hydroxyurea (2 mM), a ribonucleotide reductase inhibitor,...

Ngày tải lên: 10/08/2014, 10:20

... unequivocally show that HIV-1 was produced from CD4+ T lymphocytes Jurkat cells independently of exosomes. Thus, this study not only presents a simplified way to obtain highly puri- fied HIV-1 virions for ... [28]. This protocol is clearly not applicable to analysis of exosomes and HIV-1 virions produced from cells that express little or no CD45. Thus, this study not only presents a simpl...

Ngày tải lên: 12/08/2014, 01:21

... fluorescent dyes for detection by Odyssey Infra- red Imaging System (LI-COR Biosciences) and quantifi - cation by Odyssey software (version 2.1). Immunofluorescence microscopy Transfected or infected cells ... staining) cells. (D) The other half of the cells was analyzed for surface levels of CD317 on productively infected, p24CA-positive cells by flow cytometry. MFI values for control...

Ngày tải lên: 13/08/2014, 01:20

Ngày tải lên: 13/08/2014, 20:21