Báo cáo y học: "RhoA signaling modulates cyclin D1 expression in human lung fibroblasts; implications for idiopathic pulmonary fibrosis" ppsx

... not for citation purposes) RhoA signalling directly influences cyclin D1 mRNA and protein expression in lung fibroblastsFigure 3 RhoA signalling directly influences cyclin D1 mRNA and protein expression ... RhoA activity by inducing tyrosine phosphoryla- tion coinciding with a decrease in Rho activity [25]; accounting for its limited observed response on fibroblast cycl...

Ngày tải lên: 12/08/2014, 16:20

... imprinted only in the fibroblasts. Interestingly, 90% of the previously identified imprinted genes (18 o f 20) validated in this studywereimprintedintheprimaryfibroblastsas opposed to only 40% for ... His-MBD2b, a methyl binding pro- tein that has a high affinity for CpG methylated DNA. We then removed the non-tagged DNA, leaving only methylated fragments. Comparing the signal intensi...

Ngày tải lên: 09/08/2014, 22:24

... and cyclin D1 expression and in non small cell lung cancer. We didn’t observed also prognostic value of cyclin D1 or galectin-3 expression. But we showed higher cyclin D1 expression in galectin-3 ... transition [8,9]. Cyclin D1 plays a role in DNA repair. Cyclin D1 could bind directly RAD51, a recombinase that drives the homo- logous recombination process [10]...

Ngày tải lên: 10/08/2014, 10:21

... purposes) inflammatory cytokines, interleukin-6 (IL-6) and macro- phage inflammatory protein-2 (MIP-2) protein levels in mouse lung tissue homogenates are increased following LPS treatment as determined by ELISA ... significantly increased IL-6 and GM-CSF proteins and decreased IL-2 protein expression in lung tissue homogenates using suspension bead array. These findings correlated...

Ngày tải lên: 11/08/2014, 08:21

Ngày tải lên: 13/08/2014, 21:21

... protecting groups were em- ployed: Boc(tert-butyloxycarbonyl) for Lys and Trp, Trityl(triphenylmethane) for Gln and Asn and Pbf (2,2,4,6,7-Pentamethyldihydrobenzofurane-5-sulfonyl ) for Arg. ... × 30 µl) for 30 sec and 2 min respectively. BeadArray gene expression analysis We used an integrated genomics profiling and computational biology based strategy to identify the key gen...

Ngày tải lên: 03/11/2012, 11:44

... the human innate immune system. Proinflammatory cytokines, such as granulo- cyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), macro- phage colony-stimulating ... TLR expression with statistically significantly increased Int. J. Med. Sci. 2008, 5 6 TLR2 expression in monocytes, a trend for increased TLR4 in monocytes (IFN-γ) and...

Ngày tải lên: 08/08/2014, 16:23

... epitopes in giantin To examine the relative distribution of epitopes in giantin, we performed mapping using six overlapping partial length constructs of recombinant giantin. Expression vectors for the ... counterstained with 4′,6-diamidino-2-phenylindole. By using this approach, a total of 80 sera exhibited specific staining of the Golgi complex. Recombinant Golgi proteins Recombinant...

Ngày tải lên: 09/08/2014, 01:23

... data indicate that individual B cells of all subtypes function normally, but that humoral immunity is greatly diminished in many aged animals. We maintain that this decline in humoral immunity reflects ... mice and humans that antibody responses in the aged are lacking in quality rather than quantity, indicating minimally that B cells from aged animals are fully competent to produce a...

Ngày tải lên: 09/08/2014, 01:23

... tissues. In contrast, COX-2 is highly inducible by serum, growth factors, lipopolysaccha- rides, and cytokines, especially interleukin-1 (IL-1), in cer- tain cell types involved in inflammatory processes, ... destruction in many immunoinflammatory diseases such as RA [1-3]. The two cytokines induce, in synergy, the production of high levels of matrix metalloproteinases by synovial c...

Ngày tải lên: 09/08/2014, 01:23