... phenomena using 8-color cytokine flow cytometry, and tested the hypothesis that these two findings were causallyrelated. We analyzed cytokine profiles and memory/effector phenotypes of HIV-specific ... subjects) of the absolute number of cells for each of the 32 pheno-types, as listed at the bottom of the figure. Black bars represent the background, as mean of the absolute number of cytokine-positive ... gate.Details of the cytokine flow cytometry protocol can befound on the Maecker Lab weblog [30]. Briefly, cells werestained for 60 minutes with CD28 PerCP-Cy5.5, CD45RAPE-Cy7, CD27 APC, CD8 APC-Cy7,...