Báo cáo y học: " Differential Muc2 and Muc5ac secretion by stimulated guinea pig tracheal epithelial cells in vitro" docx

... recognition of Muc2. 45M1 monoclonal antibody recognizes Muc5ac in guinea pig tissue To examine expression of Muc2 versus Muc5ac in guinea pig tissues, we developed guinea pig- specific assays using 45M1 ... detect- able in guinea pig tracheal epithelial cells, both constitu- tively and following exposure to pro-inflammatory cytokines, is novel. Furthermore...

Ngày tải lên: 12/08/2014, 16:20

... controlled by proinflammatory cytokines and increased responses to TLR ligands. GM-CSF was shown to play an important role in inflammatory sig- naling in a model of LPS-induced lung injury. Pre- treatment ... TLR2 and TLR4 in neutrophils and monocytes. GM-CSF up-regulated expression of TLR2 and TLR4 in neutrophils and TLR2 in monocytes. TLR5 was down-regulated by i...

Ngày tải lên: 08/08/2014, 16:23

... protein-protein interactions and sites of co-binding, which were validated in Co-IP and ChIP- reChIP experiments. By reading the sequence in ChIP-DNA at polymorphic sites, we find many instances ... binding and regulatory activity of the interacting TF [33]. We did not find any evidence of protein-protein interaction between FOXA1 and FOXA3, but we cannot yet completely exclude...

Ngày tải lên: 09/08/2014, 20:21

... sites of inflammation. Aft er antigen uptake at the inflamma- tory site and maturation by inflammatory cytokines and chemokines, the DCs differentiate into inflamma- tory DCs which migrate to the lymph ... present- ing cells in general, including monocytes, macrophages and B -cells, however, other TLRs are mainly or exclu- sively expressed on myeloid or plasmacytoid DCs. Monocyte...

Ngày tải lên: 11/08/2014, 06:22

... USA). Intracellular staining for cytokines was performed using BD cytofix/cytoperm and BD perm/wash reagents with GolgiStop as recommended by BD Pharmingen. Intracellular cytokine staining was ... (PBS) and cell suspensions were generated by mechanical mincing and collagenase digestion. Debris was removed by passage through nylon mesh and cells were resuspended in 1x PBS con...

Ngày tải lên: 12/08/2014, 13:22

... NH, Song YS. XRCC1 Arginine194Tryptophan and GGH-401Cytosine/Thymine poly- morphisms are associated with response to platinum-based neoad- juvant chemotherapy in cervical cancer. Gynecol Oncol. ... PW, Hand P, Gilford J, Farrell WE, Hoban P, Fryer AA, Strange RC. Susceptibility and outcome in oral cancer: preliminary data showing an association with polymorphism in cytochrome P4...

Ngày tải lên: 25/10/2012, 10:51

... Changes in cell morphology after induction of differentiation of HL-60 cells by dimethylsulfoxide and TPA. (A) HL-60 cells cultured in RPMI for 3 days (control for B), followed by cytospin analysis and ... and staining (·1000). (B) HL-60 cells treated with 1.4% dimethylsulfoxide, followed by cytospin analysis and staining (·1000). (C) HL-60 cells in suspension after...

Ngày tải lên: 17/03/2014, 23:20

... strongly inhibited by EDTA, Hg 2+ ,andCu 2+ , but shows no loss in activity after incubation with other metals or inhibitors examined in this study. The protein retains more than 75% of its initial ... sp.RAG-1;protein purification; zymogram. Lipases are glycerol ester hydrolases (EC 3.1.1.3) that catalyze the hydrolysis of triacylglycerols to free fatty acids and glycerol. They resemb...

Ngày tải lên: 23/03/2014, 21:20

... N-terminal sequencing and amino-acid analysis. The sequence obtained, namely Lys- Ile-Glu-Glu-Gly-Lys-Leu-Val-Ile-Trp corresponds to the N-terminus of the m ature maltose-binding protein. Binding of ... m M theophylline. The arrow points to the M-A2aTr316-H10 fusion protein. Note that the main contamination, seen in lane 1, run s only m arginally above the hA2aR fusion pro tein and is t...

Ngày tải lên: 24/03/2014, 00:21

... Soybean (Glycine Max) and Saccharomyces cerevisiae (baker’s yeast), which is formulated based on traditional Chinese medicinal principles and processed by a proprietary technology developed by ... CKBM could induce cytokine release including TNF-α in these animals and inhibit tumor growth. However, further study is needed to determine how they are induced and the mechanisms t...

Ngày tải lên: 08/08/2014, 18:20