Báo cáo y học: "Regulation of chondrocyte gene expression by osteogenic protein-1" ppt

... signifi- cance of differentially expressed genes, a gene ontology analysis was conducted using DAVID/EASE [12]. Pathway analysis and classification by gene ontology Regulated genes (R > 1.5-fold, ... input-genes occur randomly within this pathway. Grouping of genes was done by computing over-rep resentati on of regulated genes in gene ontology (GO) classes [14]. Statistical...

Ngày tải lên: 12/08/2014, 15:22

... directly and by gene therapy inhibits matrix degradation in the intact degenerate human interverte- bral disc: an in situ zymographic and gene therapy study. Arthritis Res Ther 2007, 9:R83. 14. Hoyland ... non-parametric, they were then analysed by the Mann-Whitney U test. Results Effect of cytokines on matrix-degrading enzymes There was no difference in the basal levels of gene...

Ngày tải lên: 09/08/2014, 14:20

... marked by aster- isks (*). Figure 3 Gene expression pattern of chondrocytes after first passage (P1 )Gene expression pattern of chondrocytes after first passage (P1). Chondrocytes from cartilage of ... strongly (1,448-fold) after 10 to 12 days of in vitro culture to expression levels only slightly higher than the IL-1β expression of healthy chondrocytes ex vivo or of...

Ngày tải lên: 09/08/2014, 10:20

... the Prime-a -gene labeling system (Promega). Double-positive clones were purified by secondary and tertiary r ounds of screening and genomic DNA inserts were purified using poly(ethylene glycol) precipitation ... upstream of the luciferase gene, a nd the resulting plasmid constructs were transiently transfected into cultured cells. In view of the relatively low efficiency of transfec...

Ngày tải lên: 08/03/2014, 16:20

... depletion of glucose is accompanied by concerted changes in the mRNA levels for most enzymes of the central metabolism of yeast resulting in down-regulation of glycolysis and up-regulation of the ... regulation; gene expression. Microarray technologies provide the means to measure simultaneously the expression patterns of thousands of genes [1,2]. These expression data...

Ngày tải lên: 17/03/2014, 10:20

... ADP-ribosylated by diphtheria toxin [25]. ADP-ribosylation inhibits the activity of eEF2. Phosphorylation of eEF2 inhibits its activity, in translo- cation and in poly(U)-directed polyphenylalanine synthesis [26,27], ... the phosphorylation of the valyl-tRNA synthetase that associates with eEF1A/B. The available evidence suggests that phosphorylation of eEF1A/B and of valyl- tRNA...

Ngày tải lên: 23/03/2014, 21:20

... Thr70, the sites most profoundly affected by rapamycin, may be regulated by nutrients through the mTOR pathway. REGULATION OF 4E-BP1 BY AMINO ACIDS In a number of kinds of mammalian cells, amino ... the control of eIF4E (by 4E-BP1) for the activation of protein synthesis by amino acids [92]. In isolated rat heart cells (ventricular myocytes), protein synthesis is acutely ac...

Ngày tải lên: 23/03/2014, 21:20

... (0.9 ± 0.27 ng/ml per µg of hydroxyproline) and the 'pulsed EMF' group (0.9 ± 0.37 ng/ml per µg of hydroxyproline). Impairment of chondrocyte activity induced by the 3- tesla EMF is transient According ... proteoglycan synthesis of human articular chondrocytes: the role of nonenzymatic glycation. Arthritis Rheum 1999, 42:1003-1009. 48. Schafer SJ, Luyten FP, Yanagishit...

Ngày tải lên: 09/08/2014, 08:22

... Firestein GS: Cytokines in chronic inflammatory arthritis. VI. Analysis of the synovial cells involved in granulocyte-macrophage colony- stimulating factor production and gene expression in rheuma- toid ... J, Koyama Y, Majima T, Aoki Y, Ichiyama H, Ishibashi T, Minami A: Macrophage migration inhibitory factor up-regulates the expression of interleukin-8 messenger RNA in synovial...

Ngày tải lên: 09/08/2014, 10:20

... induc- tion of IκBα in the IκBα-luc transgenic mice by LPS. We demonstrated involvement of both the NF-κB and the p38 MAP kinase signaling pathways in the induction of IκBα expression by LPS. Clinically, ... regulation [9]. Induction of IκBα-luc expression in the early stage of the LPS response is consistent with a tight auto-regulation of the NF-κB signaling pathway by...

Ngày tải lên: 11/08/2014, 08:21