Báo cáo y học: "Tumor necrosis factor-alpha promotes survival in methotrexate-exposed macrophages by an NF-B-dependent pathwa" pptx

... improves survival in experimental sepsis by inhibiting lymphocyte apoptosis and reversing monocyte dysfunction Yan Zhang 1† , Ying Zhou 2† , Jingsheng Lou 3† , Jinbao Li 3 , Lulong Bo 3 , Keming Zhu 3 , ... inhibiting lymphocyte apopto sis and reversing monocyte dysfunction by modul ating cytokine production. Anti-PD-L1 antibody administration may be a promising therapeutic strategy f...

Ngày tải lên: 14/08/2014, 07:21

... therefore, investigated this proposal and the macrophage signaling pa thways underlying it. Methods: Caspase-3 activity, annexin-V binding/7-aminoactinomycin D (7-AAD) exclusion and cell-cycle analysis were ... and assayed for caspase-3 activity as described previously [20]. Flow cytometry analysis Apoptotic cells were quantitated by staining wit h annexin V-PE and 7-AAD, as specified by...

Ngày tải lên: 12/08/2014, 15:22

... joints [8]. TNF-α promotes further expression of cytokines and chemokines by synovial cells and chondro- cytes, thereby sustaining a renewal of local inflammatory mediators (reviewed in [9,10]). The ... elongation, a change that was significantly potentiated by the presence of TNF- α. The distribution and arrangement of actin filaments were analyzed by phalloidin labeling. An incr...

Ngày tải lên: 09/08/2014, 06:22

... the treatment merely blocking inflammation efficiently without interfering with the underlying pathophysiological mechanisms that for example lead to ankylosis in AS? An interesting addition to ... initially treated in a randomized placebo-controlled trial [2] and afterwards Commentary Tumor necrosis factor- αα blockade in ankylosing spondylitis: a potent but expensive anti-inflamma...

Ngày tải lên: 09/08/2014, 06:23

... expression in human monocytes is regulated by TNFα and by adalimumab, a human anti-TNFα monoclonal antibody widely used in human pathology. Our work shows differential regulation of CD36 expression by ... reduction in PPARγ activation by TNFα has already been reported in human adipocytes and hepatocytes [34,35], but has not yet been documented in human monocytes. While IL4,...

Ngày tải lên: 09/08/2014, 10:20

... concentrations in (a) serum and (b) arthritic joints and (c) TIARP mRNA and protein expression in spleens (left and middle panels) and arthritic joints (right panel) by real-time polymerase chain reaction ... immunohistochemistry using 4'-6-diamidino-2-phenylindole (DAPI), fluorescein isothiocyanate (FITC)- anti-STEAP4, and rhodamine-anti-CD68 and a merged image are shown in the...

Ngày tải lên: 09/08/2014, 14:22

... of glycosaminoglycans in the meniscus is mediated by nitrous oxide-independent aggrecanase activity in vitro Henning Voigt, Angelika K Lemke, Rolf Mentlein, Michael Schünke and Bodo Kurz Institute ... interleukin-1, tumor necrosis factor alpha, and interleukin-6 in cartilage proteoglycan metabolism and destruction. Effect of in situ blocking in murine antigen- and zymosan-induc...

Ngày tải lên: 09/08/2014, 14:22

... of 14 HBO increased AP-1 binding activity. TNF-a antibody and SP600125 inhibited the AP-1 binding activity induced by HBO, indicating that TNF-a and JNK pathway mediate the increased transcriptional ... DNA-protein binding activity induced by HBO. Exogenous addition of TNF-a significantly increased the DNA-protein binding activity. To study whether the visfatin expression induced by HBO...

Ngày tải lên: 10/08/2014, 05:21

... mounting medium with DAPI (Vector Labo- ratories via Biozol, Eching, Germany). We controlled spe- cific staining by using irrelevant primary antibodies or serum or by omitting the primary antibody. Determination ... representative staining of an RA patient is given in Figure 1. HNP1 3 were detected mainly in the synovial lining and in the directly adjacent sublining area (Figure 1...

Ngày tải lên: 12/08/2014, 14:22

... regulated by the glutathione cycle, a primary mechanism of antioxidant buffering. Buck and Chojkier [2] have shown that antioxidants and nitric oxide (NO) syn- thase blockade inhibit muscle wasting in ... cytokines and induces anorexia. Any of these effects could indirectly promote muscle wasting. Mechanisms by which TNF-α might directly stimulate catabolism are less clear. One potentia...

Ngày tải lên: 12/08/2014, 18:20