Báo cáo y học: "Human articular chondrocytes express ChemR23 and chemerin; ChemR23 promotes inflammatory signalling upon binding the ligand chemerin21-157" docx

... expression and function in chondrocytes. The objective of this study was to evaluate the expression of 15-lipoxygenase-1 and -2 in human articular chondrocytes, and to investigate the effects of their metabolites ... experiments and the PPARγ antagonist GW9662. Results Articular chondrocytes express 15-lipoxygenase-1 and -2 at the mRNA and protein levels. 13(S)...

Ngày tải lên: 09/08/2014, 14:20

... et al.: Human articular chondrocytes express ChemR23 and chemerin; ChemR23 promotes inflammatory signalling upon binding the ligand chemerin 21-157 . Arthritis Research & Therapy 2010 12:R228. Berg ... the type of ligand it binds. In the present study, we aimed to clarify whether human chondrocytes express ChemR23 and chemerin, and whether cheme...

Ngày tải lên: 12/08/2014, 15:22

... PCR specificity was confirmed by dissociation curve analysis (data not shown). IL-7 binding assay For flow cytometry analysis, chondrocytes were removed from six-well dishes by trypsin digestion and for ... Medium was analyed for sulfated glycosaminoglycan (sGAG) using the dimeth- ylmethylene blue assay and normalized for the wet weight of the tissue. (b) Total nitrite was mea...

Ngày tải lên: 09/08/2014, 10:23

... procurement and Figure 5 Collagen P4H isoenzyme gene expression in articular chondrocytes aggregatesCollagen P4H isoenzyme gene expression in articular chondrocytes aggregates. Prolyl 4-hydroxylase ... articular chondrocytes exist in a completely avascular microenvironment, and an oxygen Figure 3 Collagen types I and II, and SOX9 gene expression in human articular chond...

Ngày tải lên: 09/08/2014, 10:20

... HBSS. Mixed lymphocyte reaction (MLR) Blood samples were diluted two-fold with PBS and lay- ered onto Ficoll-Hypaque. Each tube was centrifuged at 400 g for 30 min and the lymphocytes at the interface were ... numbers of CD4+ and CD8+ T cells on pooled samples were determined on days 0, 3, and 6 post-culture by flow cytometry analysis (B). Expression of FoxP3 and CD25 were det...

Ngày tải lên: 18/06/2014, 15:20

... other cytokines (e.g. IL-8, IL-6, and leukotriene inhibitory factor), proteases, and prostaglandin E 2 (PGE 2 ) [30]. Synthesis of the inflammatory cytokines IL-1 and TNF-α and expression of their ... by the proinflammatory cytokines IL-1 and TNF-α [29–31,36]. NO may be involved in cartilage catabolism by inhibiting the synthesis of colla- gen and PG, enhancing MMP activit...

Ngày tải lên: 09/08/2014, 01:21

... examined by US, 300 by MRI, and 795 by x-ray. Ultrasonography observations in PsA and RA patients and CTRLs The observations by US in PsA and RA patients and CTRLs are listed in Table 1. In particular, ... PsA. Observations by ultrasonography, MRI, and x-ray in the MRI-examined hand The observations by US, MRI, and x-ray in the MRI-examined hand are listed in Table 2. B...

Ngày tải lên: 09/08/2014, 10:21

... purposes) Acknowledgements The authors are grateful to the staff of all the hospitals included in the study and colleagues from the Ministry of Public Health and Sanitation, the Minis- try of Medical Services and the ... (hospitals H1, H4 and H5) there was often only one nurse on duty on the paediatric ward, and even during the day it was rare for a nurse to be spe...

Ngày tải lên: 11/08/2014, 05:21

... contributions PNP and PV designed the study, conducted the field study in Uganda, and analyzed the data. PNP, PV, and ES inter- preted the data and wrote the report. All authors read and approved the final ... captivity for less than one day were released by the LRA. The LRA may have abducted as many people as they could during a raid to help them carry stolen goods...

Ngày tải lên: 11/08/2014, 17:20

... IL-1β and TNF-α are the key proinflammatory cytokines mediating cartilage degradation in patients with RA and OA. IL-1β and TNFα participate in these processes by stimulating chondrocytes and synoviocytes ... The proinflam- matory cytokines in turn stimulate articular chondrocytes and synoviocytes to produce matrix-degrading enzymes such as matrix metalloproteinases (MMPs)...

Ngày tải lên: 09/08/2014, 14:22