Báo cáo y học: "Intradiscal transplantation of synovial mesenchymal stem cells prevents intervertebral disc degeneration through suppression of matrix metalloproteinase-related genes in nucleus pulposus cells in rabbits" potx

... as: Miyamoto et al.: Intradiscal transplantation of synovial mesenchymal stem cells prevents intervertebral disc degeneration through suppression of matrix metalloproteinase-related genes in nucleus pulposus ... affected the remaining nucleus pulposus cells by inhibiting their expressions of degradative enzymes and inflammatory cytokines, resultin...

Ngày tải lên: 12/08/2014, 15:22

... The use of FGF-2 may therefore be of particular benefit in increasing the production of matrix in cartilage tis- sue-engineered in vitro. The inhibition of actin stress fibres by FGF-2 in adult ... staining predominantly the tunica intima consisting of the endothelial layer and the basement membrane, and αSMA (e, f) staining predominantly the tunica media consisting of...

Ngày tải lên: 09/08/2014, 10:23

... sur- rounding tissues [4]. Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into a variety of cell types. In the field of cell transplantation, MSCs have many advantages ... small animal imaging system is a real-time imaging system which can be used to ob- serve the distribution of cells in living animals in a real time pattern. In...

Ngày tải lên: 25/10/2012, 11:18

... Taylor F: Induction of matrix metal- loproteinase-2 and -3 activity in ovine nucleus pulposus cells grown in three-dimensional agarose gel culture by interleukin- 1beta: a potential pathway of ... vitro and in rodents by statins. Science 1999, 286:1946-1949. 18. Zhang H, Lin CY: Simvastatin stimulates chondrogenic pheno- type of intervertebral disc cells partially t...

Ngày tải lên: 12/08/2014, 11:22

... experiment to profile various inflam- matory cytokine proteins involved in the peak inflamma- tory reactions induced by ALI with E. coli instillation. Protein levels of inflammatory cytokines from ... MA, USA). Protein macroarray Each lung lysate was analyzed using a mouse cytokine array kit (Proteome Profiler™; R&D Systems, Minneapolis, MN, USA). A total of 250 μ g of lysate was...

Ngày tải lên: 12/08/2014, 13:22

... play a role in neuropathic pain. Methodology/Principal Findings: In this study, we inves- tigated the role of TLR4 in a rat chronic constriction injury (CCI) model and explored the feasibility ... protein containing extra- cellular domains with leucine-rich repeat and a cy- toplasmic signaling domain. The role of TLR4 in in- nate immune response has been well elucidated. The bi...

Ngày tải lên: 25/10/2012, 11:48

... TNFa-induced U937 cell apoptosis by maintaining mitochondrial integrity and function [43], and ricin-induced apoptosis of U937 cells by maintaining an intracellular reducing condition by acting ... Yamakawa 1 1 Department of Biochemistry, Faculty of Pharmacy, Tokyo University of Pharmacy & Life Science, Tokyo, Japan; 2 Yoneyama Maternity Hospital, Tokyo, Japan Previously, we dem...

Ngày tải lên: 22/02/2014, 07:20

... and L-6200 intelligent pump). N-Terminal sequencing was carried out using an Applied Biosystem sequencer (477A protein sequencer) on line with a phenylthiohydantoin analyser (120A analyser). Dichroic ... network stabilizing the C-terminus, is replaced by an isoleucine in BotXIVM8- 10. Finally, the length and amino-acid composition of the C-termini of LqhaIT and BotXIVM8-10 are signifi- c...

Ngày tải lên: 08/03/2014, 23:20

... Ca 2+ ] i spiking not by increasing the InsP 3 level but by potentiating InsP 3 receptor-mediated Ca 2+ release. This may explain why the Ca 2+ response with thimerosal is only partially inhibitable by P ... Ca 2+ responses in platelets, but in this case in the absence of InsP 3 generation. Throm- bin-induced spiking was acutely abolished by inhibiting phospholipase C or elevating int...

Ngày tải lên: 17/03/2014, 17:20