Báo cáo y học: " Cryptococcus neoformans induces IL-8 secretion and CXCL1 expression by human bronchial epithelial cells" doc

... purposes) Respiratory Research Open Access Research Cryptococcus neoformans induces IL-8 secretion and CXCL1 expression by human bronchial epithelial cells Loïc Guillot 1 , Scott F Carroll 1,2 , Mohamed Badawy 4 ... genes by C. neoformans in human BEAS-2B cells using an oligonucleotide microarray and confirmed the hybridization data by conventional and rea...

Ngày tải lên: 12/08/2014, 15:21

... induced by EGCG likely led to the decrease of cell migration. Our results are emphasized by those previ- ously reported by Ermakova et al [40] who demonstrated that vimentin is a target for EGCG by ... was assessed by using 2D and 3D models of cell culture. We also studied the effects of EGCG on proteinases expression by RT-PCR analysis. By immunocytochemistry, we analyzed a...

Ngày tải lên: 12/08/2014, 15:21

... morphogenetic and functional defects by promoting the death of erythrocytes and the endocardium in zebrafish embryos Yi-Ting Wu 1 , Che Yi Lin 1 , Ming-Yuan Tsai 2 , Yi-Hua Chen 3 , Yu-Fen Lu 3 , ... hemoglobin, and both non-enzymatic and enzymatic antioxidants provide erythrocytes with an Figure 5 Both dicoumarol and BAPTA-AM rescued the erythrocyte-deficiency in circulation and...

Ngày tải lên: 10/08/2014, 10:20

... 2'O-methyl RNA (5 residues), and were synthesised by Proligo (Hamburg, Germany) [14]. Synthetic siRNAs (CAT, GFP, LacZ) were synthesized by Xeragon using 29- O-(tri-isopropyl) silyloxymethyl chemistry ... implying that asODN function mainly in the nucleus, where they bind to mRNA target sequence specifically by forming Watson-Crick base pairs. The mRNA/asODN hybrid is subsequentl...

Ngày tải lên: 12/08/2014, 16:20

... PE-conjugated IgG1iso- type control antibody at 4°C for 30 min and analyzed by flow cytometry using a FACScan flow cytometer and Cell Quest software (Becton Dickinson, Mountain View, CA). The expression rate ... activity and impairs lipopolysaccharide (LPS) induced upregulation of AP-1 by, in part, by directly targeting the Jun B and Jun D transcripts. Dose dependent down- regu...

Ngày tải lên: 10/08/2014, 21:23

... similar inhibitory pat- tern (85% by SP600125, 21% by SB203580 and 60% by PD98059, respectively) as the fresh segments, although SB203580 had significantly less inhibitory effects (Fig. 8B). In segments ... choline acetyltransferase (ChAT) and house keeping gene β-actin were designed by using Prime Express ® 2.0 software (Applied Biosystem, Forster city, CA, USA) and synthe- si...

Ngày tải lên: 12/08/2014, 15:20

... mastocytoma P-815 was readily phosphorylated by CaM kinase II. Although the TPH from P-815 cells was easily phosphorylated by this kinase, its enzymic activity was not altered by the phosphorylation ... of Oxygen Activation (Hayaishi, O. ed.), pp. 285–369. Academic Press Inc., New York and London. 2. Hosoda, S. & Glick, D. (1965) Biosynthesis of 5-hydro- xytryptophan and 5-hydro...

Ngày tải lên: 08/03/2014, 16:20

... other hand, an impaired DNA cleavage observed in the cleavage assay should be reflected by inhibition of the reaction catalysed by htopo I rather than by inhibition of the movement of the enzyme ... fragments produced by the endonucleases were further cleaved by htopo I (Fig. 4, lanes 2 and 6). However, it is clearly visible that SF2/ASF only slightly inhibited the cutting of DNA...

Ngày tải lên: 24/03/2014, 03:21

... difficulties, chronic psychogenic and somatic pain, frequent hospital admissions, hospital dependency and dependency on oxygen. This metaphori- cally and literally suffocating disease status may explain the ... (categorized as such by respiratory symptoms evaluation and functional tests using medical criteria) [17]. Chronic disease and comorbidity with anx- iety and depression a...

Ngày tải lên: 08/08/2014, 23:20

... with control siRNA, as indicated by an enhanced secretion of proinflammatory cytokines such as IL- 6 and TNF-α and by the enhanced phosphorylation of STAT1, IκB and JNK upon stimulation. Antigen ... recruit and activate STATs (especially STAT5) has been proposed as the mechanism by which CIS and SOCS2 inhibit cytokine signaling [11,12]. CIS is induced by cytokines that act...

Ngày tải lên: 09/08/2014, 06:23