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Báo cáo khoa học: "Description of the Infection Status in a Norwegian Cattle Herd Naturally Infected by Mycobacterium avium subsp paratuberculosis " potx

Báo cáo khoa học:

Báo cáo khoa học: "Description of the Infection Status in a Norwegian Cattle Herd Naturally Infected by Mycobacterium avium subsp. paratuberculosis." potx

... UnitedStates (Pavlik et al. 2000, Whipple et al. 1990).These findings indicate that the same M. a. paratuberculosis strain has infected goats and cattle. Paratuberculosis in a Norwegian cattle herd ... healthand had an average milk and meat production.No clinical signs of paratuberculosis werenoted in any animals at the time of culling.Serological examinationsSerological examination was ... paratuberculosis; paratuberculosis; cattle; diagnosis; IFN-γActa vet. scand. 2005, 46, 45-56.Acta vet. scand. vol. 46 no. 1-2, 2005Description of the Infection Status in a Norwegian Cattle Herd Naturally Infected...
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Báo cáo khoa học: Prediction of missing enzyme genes in a bacterial metabolic network Reconstruction of the lysine-degradation pathway ofPseudomonas aeruginosa doc

Báo cáo khoa học: Prediction of missing enzyme genes in a bacterial metabolic network Reconstruction of the lysine-degradation pathway ofPseudomonas aeruginosa doc

... lysine-degradation pathway:l-lysine fi 5-amino pentanamide fi 5-amino pen-tanoate fi glutarate semialdehyde fi glutarate andl-lysine fi cadaverine fi 1-piperideine fi 5-aminopentanoate fi glutarate semialdehyde ... archaea, and thereis also variation within bacteria. We also focused on the lysine-degradation pathway because the substratesand intermediates of the pathway are structurally sim-ple, and so the ... reconstruct the lysine-degradation pathway in P. aeruginosa using EC number information. As a result, we identified PA0266 as a putative 5-aminovalerate aminotransferase(EC 2.6.1.48) and PA0265 as a putative...
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Báo cáo khoa học: Involvement of the V2 receptor in vasopressin-stimulated translocation of placental leucine aminopeptidase/oxytocinase in renal cells pdf

Báo cáo khoa học: Involvement of the V2 receptor in vasopressin-stimulated translocation of placental leucine aminopeptidase/oxytocinase in renal cells pdf

... reagents thatincrease intracellular cAMP also activate the translocation of P-LAP, indicating that AVP stimulates the transloca-tion of P-LAP from the intracellular compartment to the plasma ... translocation of P-LAP to the basolateral plasma membrane. Translocated P-LAPmight then facilitate the degradation of excessive vaso-pressin, acting as a negative feedback mechanism of AVPaction ... dose-dependent manner. Kinetic analysis revealed that the increase in the surface level of P-LAP was detectable at30 min after AVP addition (Fig. 3C). We also examined the AVP-mediated increase in the cell...
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Báo cáo khoa học: Role of the structural domains in the functional properties of pancreatic lipase-related protein 2 pot

Báo cáo khoa học: Role of the structural domains in the functional properties of pancreatic lipase-related protein 2 pot

... that the proteins possessing at least one of the twodomains of hoPL are more sensitive to interfacialdenaturation. The involvement of the C-terminaldomain in the interfacial denaturation of ... whatever the nature of the N-terminal domain.Moreover, the C-terminal domain of hoPLRP2 wasnot able to bind colipase even in the presence of the PL N-terminal domain. In uence of NaTDC and colipase ... wereinvolved in the inactivation of the protein at the water–triglyceride interface. The inactivation of N2Cc in the absence of bile salts was prevented by prioraddition of colipase, suggesting that N2Cc...
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Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot

Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot

... within the RAIA variant, which already shows a reduction in basal and insulin-stimulated phosphoryla-tion at Thr36 ⁄ 45 (Fig. 2A) . Mutation of the arginineto lysine in the RAIA variant (to create ... anyeffects of other mutations. Thus, we tested the impor-tance of the proline residue in a variant of 4E-BP1 in which the arginine was mutated to alanine (AAIP,which shows modestly decreased basal and ... maintained by the amino acids in the medium A BCDFig. 2. Assessment of the phosphorylation of 4E-BP1 mutants containing variants of the RAIP motif. (A D) Wild-type 4E-BP1 (RAIP) or the indicated...
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Báo cáo khoa học: Characterization of the flavin association in hexose oxidase from Chondrus crispus pot

Báo cáo khoa học: Characterization of the flavin association in hexose oxidase from Chondrus crispus pot

... 2006 DANISCO 2699 of this action is not known. The main reason for thisis the lack of a crystal structure and the lack of infor-mation regarding the association and placement of FAD in the holoenzyme. ... 6-S-cysteinylriboflavin as the starting materialand not enzyme-bound 6-S-cysteinyl, 8a- N1-histidyl-riboflavin. The pK a value of the hydrolysate of HOXcontaining the histidyl–cysteinylriboflavin moiety ... A ˚, based on 388 Ca-atoms. Accord-ing to the model, the N-1 atom of His79 is located2.4 A ˚from the 8a C atom of the isoalloxazine ring in FAD, and the S atom of Cys138 is located 1.8 A ˚from...
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Báo cáo khoa học: Distribution of the extrinsic proteins as a potential marker for the evolution of photosynthetic oxygen-evolving photosystem II ppt

Báo cáo khoa học: Distribution of the extrinsic proteins as a potential marker for the evolution of photosynthetic oxygen-evolving photosystem II ppt

... The psbP gene is pre-sent in some of the algae in the red lineage but may belost in other part of the red lineage. In the green lin-eage containing Prasinophyceae, Euglenophyta, greenalgae ... that Prasinophytes also contain PsbQ. The thylakoid membranes of P. parkeae and E. gracilis didnot react with any antibodies against the red algal andcyanobacterial extrinsic proteins (lanes ... which plays an important role in maintain-ing the stability and activity of the manganese clusteris present in all of the oxygenic photosynthetic organ-isms. In contrast, the other extrinsic...
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Báo cáo khoa học: Importance of the gating segment in the substrate-recognition loop of pyranose 2-oxidase pptx

Báo cáo khoa học: Importance of the gating segment in the substrate-recognition loop of pyranose 2-oxidase pptx

... found in the binding, but rather is the result of a differential destabilization of the transitionstate for Glc in the variant. An already poor transi-tion-state stabilization of Gal in the wild-type ... conformational changes at the Asp452backbone, and a small rotation of the Leu545side chain in response to minor side-chain adjustments of the nearby Glu542. All of these changes appearremote and ... denatured at 100 °C (10 min). Denatured proteinwas partioned from the supernatant by centrifugation, and the flavin absorption spectra of the supernatants wererecorded.ThermoFAD analysis The ThermofluorÒ-based...
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Báo cáo khoa học: Regulation of the muscle-specific AMP-activated protein kinase a2b2c3 complexes by AMP and implications of the mutations in the c3-subunit for the AMP dependence of the enzyme docx

Báo cáo khoa học: Regulation of the muscle-specific AMP-activated protein kinase a2b2c3 complexes by AMP and implications of the mutations in the c3-subunit for the AMP dependence of the enzyme docx

... substitutions in the AMP-binding Bateman domains of c3.We demonstrate that AMP-activated protein kinase a2 b2c3 trimers areallosterically activated approximately three-fold by AMP with a half-maximal ... upstream kinases.AbbreviationsAICAR, 5-aminoimidazole-4-carboxamide-1-b-D-ribonucleoside; AMPK, AMP-activated protein kinase; AMPKK, AMP-activated protein kinasekinase; CAMKKb,Ca2+⁄ calmodulin-dependent ... concentration of AMPcausing a half-maximal increase in the total activity aswell as the fold stimulation by AMP [42–46]. Ourexperiments show that AMPK a2 b2c3 trimers are acti-vated by AMP with a...
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Báo cáo khoa học: Characterization of the serotoninergic system in the C57BL/6 mouse skin potx

Báo cáo khoa học: Characterization of the serotoninergic system in the C57BL/6 mouse skin potx

... specific strains the reaction can either be mediated by NAT-1 or AANAT, or by both NAT-1 and AANAT. The C57BL/6 mouse strain has undetectable production of melatonin in the pineal gland, and very ... (Shimadzu,Japan). Quantitative determination of N-acetylserotoninwas made by comparing the observed peak areas with the peak areas of known concentrations of the NAS standard.Hydroxyindole-O-methyl-transferase ... Yamazoe, Y., Nishikawa, T. & Kato,R. (1988) Properties of cutaneous acetyltransferase catalyzingN- and O-acetylation of carcinogenic arylamines and N-hydro-xyarylamine. Biochem. Pharmacol....
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