Báo cáo y học: "Phenotypic alterations in type II alveolar epithelial cells in cell mediated lung inflammation" pdf

... II A B C D A B C D A´ B´ C´ D B´ C´ D´ ´ B Maclura pomifera 98% Intracellular cytokine staining in CD4 + T cellsFigure 2 Intracellular cytokine staining in CD4 + T cells. CD4 + T cells from the lung or bronchial lymph nodes (BLN) from ... 2 0 1000 2000 3000 4000 5000 6000 1 day 3 day 6 day Gelsolin Array 1 Gelsolin Array 2 Tubulin Array 1 Tubulin Array 2 Vimentin Array 1 Vimenti...

Ngày tải lên: 12/08/2014, 15:20

... confirming the absence of significant contaminant by nucleotide. Assay of enzyme activity The activity of the shikimate kinase was determined by a double coupled assay involving pyruvate kinase ... performed essentially as described in our previous studies on type II dehydroqu- inase [28]. To study the extent of unfolding of SK, the enzyme was routinely incubated in buffer D in the st...

Ngày tải lên: 08/03/2014, 23:20

... DR4-(DRB1*0401)-restricted T cells in response to type II collagenProliferation of DR4-(DRB1*0401)-restricted T cells in response to type II collagen. A panel of analog peptides containing amino acid sub- stitutions ... by T cells An important characteristic of an altered peptide ligand is its ability to alter the cytokine profile of responding T cells. We therefore determi...

Ngày tải lên: 09/08/2014, 08:22

... flow cytometric analysis of cells: CD4–APC (APC = allophycocyanine), type cytokines [6–8]. In localized WG a higher CCR5 expression on memory T cells might favor the recruitment of Th1 -type effector ... predominantly T helper type 1 (Th1) -type cytokines in localized WG [3], a shift towards stronger Th2 -type cytokine expression is found in granulo- matous lesions of the resp...

Ngày tải lên: 09/08/2014, 01:21

... (reviewed in [1]). In the synovial joint, infiltrating T cells are predominantly of the CD4 + pheno- type and are often found in the proximity of B cells and macro- phages. These T cells could either ... CD4 in the three-colour stainings to identify CD4 + T cells. The density of CD4 + CD28 null T cells were calculated by dividing the number of CD4 + CD28 null T cells...

Ngày tải lên: 09/08/2014, 10:21

... splenic marginal zone B cells harboring a prediversified immunoglob- ulin repertoire. Blood 2004, 104:3647-3654. 12. Tangye SG, Good KL: Human IgM + CD27 + B cells: memory B cells or “memory” B cells? ... with infliximab could be an increased formation or circulation of pre-switch memory B cells by interfering with follicular dendritic cell function and germinal centre formation in...

Ngày tải lên: 09/08/2014, 14:22

... may lead to increased numbers of memory B cells and plasma cells, and a continued presence of memory B cells, as shown in the current smokers in our study. Because this increase in memory B cells ... isolated cells were counted using a Sysmex pocH-100i cell counter (Sysmex, Roche, Germany). Cells were used for flow cytometry and immu- nocytochemical staining on cytospins. Fl...

Ngày tải lên: 12/08/2014, 14:20

... to obtain alveolar cells. Contaminating red blood cells were eliminated by hypot- onic lysis for 30 seconds with double-distilled water. Remaining cells were washed twice in PBS. FACS analysis Surface ... (FCS, Biochrom, Berlin, Germany). The number of living cells was determined using the CASY ® 1 Cell Counting System (Schärfe Systems, Reutlingen, Germany) and AMs were incubate...

Ngày tải lên: 12/08/2014, 16:20

... motif plays in CD94/NKG2A expression in CD8 + T lymphocytes and NK cells from healthy donors and HIV-1 infected patientsFigure 1 CD94/NKG2A expression in CD8 + T lymphocytes and NK cells from ... very significantly decreased in HIV-1-infected patients in comparison with non-infected controls. Within the HIV infected patients, the proportion of CD8 + T lymphocytes and NK cell...

Ngày tải lên: 13/08/2014, 05:22

... migrated cells, cell nuclei were stained with Giemsa (Dade Behring). Attached cells were counted manually in five random microscopic fields. Angiogenesis assay An angiogenesis assay plate (BD Pharmingen) ... (Braun, Melsungen, Germany) for premedication, general anesthesia was induced by intravenous injection of 1 mg/kg etomidate (Braun). Anesthesia was maintained by continuous intrave...

Ngày tải lên: 13/08/2014, 18:22