Báo cáo khoa học: "Wet Belly in Reindeer (Rangifer tarandus tarandus) in Relation to Body Condition, Body Temperature and Blood Constituents" pdf

... Trp232 and Phe282 in caspase-7). The in ammatory and initiatorcaspases have larger S2 subsites due to the substitutionof Tyr230 with Val and tolerate bulkier side chains.The S2 subsites in caspase-3 ... ofTyr230 by Val in inflammatory and initiator caspaseswas suggested to account for the accommodation oflarge P2 side chains. Furthermore, screening of peptideinhibitors based on amino acid positional ... dyad(Cys186 and His144) and the residues anchoring theaspartate residue in the P1 position (Arg87, Ala145 and Gln184) which are in the p20 chain. The inhibitors bindthe two active sites in the heterotetramer...

... seed-storageproteins are composed of the two major globulinsglycinin and b-conglycinin. They are folded and assembled into trimers in the ER, and then trans-ported and deposited in the protein ... proteins belonging to group IV, GmPDIS-1 and GmPDIS-2, and demon-strated that GmPDIS-1 is associated with proglycinin in the ER [20]. However, involvement of the otherPDI proteins in the folding ... GmPDIM and changes in its expression dur-ing seed development are described. In addition, ourdata suggest that GmPDIM and proglycinin or b-con-glycinin associate during the course of the foldingprocess.ResultscDNA...

... phloem and sapwood of individual pines belonging to 3species (Pinus contorta, Pinus ponderosa and Pinus monticola) to inoculation with Ophiostoma cla-vigerum and injection ... with chitosan, a proteinase inhibitor-inducing factor and a control buffer, wasinvestigated quantitatively and qualitatively. The total quantity of monoterpene in the reactive ... of Cook and Hain (1985), with Loblol-ly pine and 2 strains of Ophiostoma minus, and of Delorme and Lieutier (1990) withScots pine and 3 different fungi and 1 bee-tle...

... a-synuclein with100 lM neurotoxin; lane 2, a-synuclein with 200 lM neurotoxin;lane 3, a-synuclein with 100 lM neurotoxin and 50 lM dopamine;lane 4, a-synuclein with 200 lM neurotoxin and 50 ... with 100 lM neurotoxin; lane 3, with 200 lM neu-rotoxin; lane 4, with 100 lM neurotoxin and 50 lM dopamine;lane 5, with 200 lM neurotoxin and 50 lM dopamine. (D) Lane M,prestained molecular ... MPP+ and (D)200 lM MPP+. Samples are a-synucleinalone (s, solid line), in the presence of100 lM neurotoxin (•, dotted line), in thepresence of 200 lM neurotoxin ( , dottedline) and in...

... time-consuming and greatly in uenced by individual handlers. IntelliCages areautomated learning cages where animals carryingtransponders are housed in groups and trained in learning corners. Each individual ... important in minimizing the in uence ofconfounding factors arising from inherent problemsassociated with breeding APP transgenic mice and maintaining stable phenotypes. We would argue that asingle ... [95]. In a similar strategy, thepathogenic interaction between Ab and a-synucleinwas investigated by crossing PDGF–a-synuclein and APP-SweInd, which led to a 1.6-fold increase in a-synuclein inclusions...

... theEWV614motif in MT5-MMP ICD as comprising animportant sequence for the interaction with the PDZproteins: Mint-3, AMPA binding protein and gluta-mate receptor interacting protein (GRIP). It ... GRASP55 binding.MT1-MMP ICD binds to PDZ2 domain and region 3 of GRASP55GRASP55 contains two non-overlapping and structur-ally independent PSD-95/SAP90 Drosophila septatejunction protein discs-large ... restricted to 3% of maximum(< 0.3 mW at focal plane) to minimize phototoxicity and bleaching. Coverslips (PeCon GmbH, Erbach, Germany)were placed in a POC-R imaging chamber (PeCon GmbH)containing...

... proteinutilizing the principle of protein–dye binding. AnalBiochem 2, 248–254.38 Bauer R & Rupe CO (1971) Use of syringaldazine in aphotometric method for estimating ‘free’ chlorine in water. ... pH 4.5 and 8.0, the LAC3 and LAC535enzymes appear undistinguishable from a kinetic pointof view. On the other hand, the LAC131 and LAC232enzymes appear to be more tolerant to alkaline pH, ... bias in the selec-tion of recombination points. It has been suggestedthat optimal recombination points allow swapping ofstructural blocks [19], and combination of large pro-tein fragments in...

... Igepal, 1% inhibitor coc-tail). To remove biotinylated surface proteins, cell lysatewas added to a streptavidin agarose resin and incubated for30 min. After centrifugation, only unlabelled intracellularproteins ... laser-scanning inverted microscope (Carl ZeissInternational, Go¨ttingen, Germany).Production of conditioned media for MMP and TIMP determination To determine the secretion of MMP-2 and TIMP-1 into ... were attached to. Hence, the reduction in MT1-MMP and activeforms of MMP-2 could not be attributed to changes in the actin cytoskeleton. This adds to previous investiga-tions showing that these...

... little apoptosis, according to previousobservations [14], and an insignificant G1 peak.Ionizing radiation, similar to the effect of cytotoxicdrugs, induces DNA damage (either directly or indi-rectly), ... intact cells and TG signals in lipid extracts To clarify whether intensity changes of ML signalswere mainly related to changes in TG concentration, to differences in chain mobility due to structuralchanges, ... the connectivity of the vinyl protons (at5.35 p.p.m.) to the allylic protons (at 2.05 p.p.m.) in monounsaturated chains and to the bis-allylic protons(at 2.80 p.p.m.) in polyunsaturated fatty...