Báo cáo y học: "Human metapneumovirus induces more severe disease and stronger innate immune response in BALB/c mice as compared with respiratory syncytial virus" pps

... Access Research Human metapneumovirus induces more severe disease and stronger innate immune response in BALB/c mice as compared with respiratory syncytial virus Barbara Huck 1,4 , Dieter Neumann-Haefelin 1 , ... more severe disease associated with higher levels of cytokines and a much stronger NK cell response. These findings indicate importa...

Ngày tải lên: 12/08/2014, 15:20

... re- sulted in increased mutations in the non-c od ing D-loop as well as the coding genes N D 1 and C OI o f mtDNA of gastric cells [12]. The increase in the num- ber of mutations was mainly attributed ... activity and ex- pression of base excision repair and mismatch repair are down-regulated both i n v i t r o and in vivo. Moreo- ver, Hp induces genomic instability...

Ngày tải lên: 25/10/2012, 11:18

... A clear increase in mean fluorescence intensity of BEAS-2B cells was observed following incubation with labeled cryptococci that were grown at 20° or at 37°C, verifying stable fungal binding in both ... cryptococci exhibited a stable asso- ciation with BEAS-2B cells that was resistant to enzymatic treatment with trypsin as well as various physical manip- ulations including washi...

Ngày tải lên: 12/08/2014, 15:21

... penicillin-streptomycin (Eurobio) and interleukin-2 (Boehringer-Mannheim, Germany). Antibodies, plasmids and cell lines The following mouse monoclonal antibodies against human proteins and matching ... selectively infected by HIV-1 and releases infectious virions. Infected cells were mainly localized within the stroma and were rarely found within the epithelium, whether hyperplasic...

Ngày tải lên: 13/08/2014, 05:21

... with the 110 kDa α5β1 integrin-binding fibronectin fragment or with IL-1, IL- 6, or IL-7. Cytokines and matrix metalloproteinases released into the media were detected using antibody arrays and ... enzyme-linked immunosorbent assay; GAG = glycosaminoglycan; IL = interleukin; MMP = matrix metalloproteinase; OA = osteoarthritis; PCR = polymerase chain reaction; PYK = proline-rich tyros...

Ngày tải lên: 09/08/2014, 10:23

... analysis were suggestive of malignancy. Flow Cytometry The expression markers on T cells from MPE and blood were determined by flow cytometry after surface stain- ing or intracellular staining with ... shihuanzhong@sina.com † Contributed equally 1 Department of Respiratory Diseases, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong...

Ngày tải lên: 12/08/2014, 13:22

... was also supported by phylogenetic analysis. In line with these bioinformatic analyses, FIV replication was efficiently inhibited in acutely infected cell cultures by three investigational INSTIs, ... Jr, Pommier Y: Probing HIV-1 integrase inhibitor binding sites with position-specific integrase-DNA cross-linking assays. Mol Pharmacol 2007, 71:893-901. 22. Savarino A: In- Silico doc...

Ngày tải lên: 13/08/2014, 05:22

... the synthetic tri- peptide substrate N-[3-(2-furyl)acryloyl] phenylalanylglcylglcine (FAPGG). FAPGG is hydro- lyzed by ACE to furylacryloylphenylalanine (FAP) and glycylglycine. Hydrolysis ... activity of the NO system with an increase in SBP connoting augmented system activity. Cytokine Assay: Various cytokines were measured by ELISA methodology using kits from the following sourc...

Ngày tải lên: 26/10/2012, 08:57

... the bystander gene has also been retained in two copies in the compared (fish) species, and that only one of the two copies of the bystander gene is still in synteny with the corresponding copy of ... 3:53-64. 51. Kasahara M, Naruse K, Sasaki S, Nakatani Y, Qu W, Ahsan B, Yamada T, Nagayasu Y, Doi K, Kasai Y, Jindo T, Kobayashi D, Shimada A, Toy- oda A, Kuroki Y, Fujiyama A, S...

Ngày tải lên: 09/08/2014, 20:20

... the flow cytometry assays, directed the SFGH Clinical Immunology Laboratory that performed the flow cytometry assays and CSF CCL2 ELISA assays, and analysed and interpreted flow cytometry data. ... clinical practice. Recently, the antibiotic, mino- cycline, has been proposed as a candidate therapy in this broad class. Minocycline has been shown to reduce lentivirus infection and i...

Ngày tải lên: 10/08/2014, 05:22