Báo cáo y học: "Innate immunity triggers IL-32 expression by fibroblast-like synoviocytes in rheumatoid arthritis" ppsx

... reactivity VEGF expression was invariably cytoplasmic, and usually strong and diffuse in the synovial lining cells of RA and OA. Normal synovium reacted only weakly with VEGF anti- bodies. PD-ECGF expression ... K: Interleukin-18 and interleukin-12 synergize to stimulate the production of vascular permeability factor by T lymphocytes in normal subjects and in patients with minimal...

Ngày tải lên: 09/08/2014, 01:21

... G, Melino G, Lee SC, Steinert PM: Protein unfolding by peptidylarginine deiminase. Substrate specificity and structural relationships of the natural sub- strates trichohyalin and filaggrin. J ... bound by inflammatory cells via their Fcγ receptors. This will activate these cells and cause the release of extra proinflammatory cytokines. D Various polymorphisms in proinflammatory cytokine...

Ngày tải lên: 09/08/2014, 01:23

... about 3 days, they will die in large numbers at the site of inflammation, which may trigger histone citrullination. In summary, although many different citrullinated proteins may be present in the ... detection of citrullinated proteins [30]. This is illustrated by the fact that the staining could be completely abolished by competition with free L-citrulline [29]. In addition, st...

Ngày tải lên: 09/08/2014, 01:23

... citrullinated proteins by the anti-modified citrulline kit was mainly confined to the subsynovium. (b) No staining was visible on the control. (c) Staining produced by the anti-α-enolase antibody ... extensively deiminated in vitro. This is demonstrated by the uniform migration of deiminated enolase at a pI of 5 and by the replacement of arginine by citrulline in all the peptid...

Ngày tải lên: 09/08/2014, 07:20

... request. Genotyping protocol SNPs were genotyped using Sequenom MassArray genotyp- ing technology, according to manufacturer's instructions, whereby the genomic sequence containing the SNP ... autoimmu- nity [1,2]. Recently convincing proof of this hypothesis has been provided by the association of the missense single nucle- otide polymorphism (SNP; rs2476601) in the protein tyrosine...

Ngày tải lên: 09/08/2014, 08:22

... pannus and contribute to joint destruction by secretion of pro-inflammatory cytokines and tissue-degrading enzymes [3]. Recently, microarray techniques employing hybridization of biological samples ... protease inhibitors, resulting in enhanced proteolytic activity [41] and controls cell proliferation by processing the insulin-like growth factor-binding protein-1 [42]. MMP-11 is, theref...

Ngày tải lên: 09/08/2014, 10:20

... and synovial fluid was analysed by four-colour flow cytometry (FACSCalibur instrument; Becton Dickinson Immunocytome- try Systems, San Jose, CA, USA) in peripheral blood mononu- clear cells and synovial ... T cells in the synovial fluid by screening synovial fluid samples from 128 patients by flow cytometry. CD4 + CD28 null T cells could be detected in synovial fluid at a median...

Ngày tải lên: 09/08/2014, 10:21

... may be relevant to RA. Increased significance in the combined samples in the study of Jacq and colleagues, corroborated by data from a genome-wide study, suggests that this association may indeed ... newly found associations would be greatly aided by the deposition of genotypes and detailed phenotype data in public databases such as dbGaP [13] for use in (disease sub-type-specific...

Ngày tải lên: 09/08/2014, 10:21

... 1980s, the stress systems including the sympathetic nervous system (SNS) and the hypothalamic–pituitary–adrenal axis were thought to play a proinflammatory role by supporting the immune response. ... very similar effect on serum or supernatant levels (in cell culture experiments) of proinflammatory cytokines (Table 1). Since stress in healthy subjects can lead to an increase of serum cyt...

Ngày tải lên: 09/08/2014, 14:21

... these cells in the inflamed synovia and that blocking the effects of TNF decreases trafficking into and retention of B cells in the synovia, thereby increasing their proportion in the peripheral ... with infliximab could be an increased formation or circulation of pre-switch memory B cells by interfering with follicular dendritic cell function and germinal centre formation in second...

Ngày tải lên: 09/08/2014, 14:22