Báo cáo y học: " Immature cell populations and an erythropoiesis gene-expression signature in systemic juvenile idiopathic arthritis: implications for pathogenesis" ppt

... sets, many representing fetal and embryonic hemoglobins (h emoglobins δ, μ, g, and θ), erythrocyte structural proteins (erythrocyte membrane protein band 4.2), surface proteins (glyco- phorins A, ... Further- more, an expansion of precursor cells and upregulation of an erythropoiesis gene-expression signature occurred predominantly in individuals with sJIA and anemia. Stri...

Ngày tải lên: 12/08/2014, 14:22

... multiple sclerosis, and ankylosing spondylitis [60–64]. In general, these cells were clonally expanded and included autoreac- tive T cells, implicating them directly in the pathogenesis of these diseases. In ... Fulbright for assistance in manuscript prepara- tion and for preparing the graphics and Linda H Arneson for secretarial support. Arthritis Research & Therapy...

Ngày tải lên: 09/08/2014, 01:23

... onfirmed by Western blotting using anti-V5 (Fig. 4A) and S12 anti-LMP1 antibody (Fig. 4B). Expression was demonstrated in LMP1 transfectants (Fig. 4A, lanes 2 and 4; Fig 4B, lanes 3 and 5) as ... polymerase (Finnzyme, Finland). PCR cycle condition was: 1 cycle at 98°C for 30 sec, followed by 30 cycles at 98°C f or 10 sec, 61°C for 30 sec, 72°C for 15 sec and a final cycle at 7...

Ngày tải lên: 10/08/2014, 05:21

... Data were collected for 10 4 lymphocytes. Positively and nega- tively stained populations were calculated by quadrant dot plot analysis determined by isotype controls. Statistical analysis A non-normal ... ligand for the CC- chemokine receptors CCR1, CCR3 and CCR5, has recently been demonstrated in pulmonary granulomatous lesions in WG. The chemokine is expressed mainly in mac...

Ngày tải lên: 09/08/2014, 01:21

... described by Nanakumar and coworkers [12], scoring of animals was done blindly using a scoring system based on the number of inflamed joints in each paw, inflammation being defined by swelling and redness. ... Germany) and transferred to a S-VHS video system for subsequent offline analysis. Microcirculatory analysis For quantitative offline analysis a computer-assisted microcir- c...

Ngày tải lên: 09/08/2014, 06:23

... experiments and was primary author of the manuscript. KS performed the hemolytic assays and assisted in interpreting the data. RAW generated the knockout mice, assisted in interpreting the data, and ... 1983, 209:847-856. 9. Lappin DF, Whaley K: Interferon-induced transcriptional and post-transcriptional modulation of factor H and C4 binding- protein synthesis in human monocyt...

Ngày tải lên: 09/08/2014, 10:21

... N(epsilon)-(carboxymethyl)lysine in human tissues in diabetes and aging. J Clin Invest 1997, 99:457-468. 37. Le Maitre CL, Freemont AJ, Hoyland JA: The role of interleukin-1 in the pathogenesis of human intervertebral ... Hoyland JA, Freemont AJ: Interleukin-1 receptor antagonist delivered directly and by gene therapy inhibits matrix degradation in the intact degenerate human inte...

Ngày tải lên: 09/08/2014, 10:23

... variants. Materials and methods hzAnalyzer hzAnalyzer is an R [19] package t hat uses Java classes for detecting runs of contiguous homozygous genot ypes and R functions for quantifying the frequency and extent ... extent and frequency of homozygous segments with haplotypes underlying ext AUC peaks. Analysis of the consistency of the homozygous extent distribution and length...

Ngày tải lên: 09/08/2014, 22:23

... final version of the manuscript. GA, RB-D and AR helped in the data analysis and interpretation. JJ and JC supervised the laboratory determinations. All authors have read and approved the final ... presumably increasing SDF-1 values. SDF-1 plays a significant role in HIV infection because it is the natural ligand for CXCR4 which is used by HIV T- tropic strains to enter into the...

Ngày tải lên: 10/08/2014, 05:21

... the study, designed and optimized the antibody panel, performed the experiments, analyzed the data and prepared the manuscript. CMT and SB aided in study design, and critical review of manuscript. ... anti- CD38, allophycocyanin (AP C) conjugated anti-HLA-DR, and peridinin chlorophyll protein (PerCP) anti-CD3 anti- bodies (BD Biosciences, Mississauga, Canada) for 30 min- utes i...

Ngày tải lên: 10/08/2014, 05:22