Báo cáo y học: " Differential expression of Toll-like receptors on human alveolar macrophages and autologous peripheral monocytes" pdf

... Amebocyte Lysate Assay (Pyro- gentPlus, Cambrex, Walkersville, MD). Stimulation of monocytes and bronchoalveolar cells with TLR ligands To assess ligand-induced TLR expression of monocytes and bronchoalveolar ... 0.9 Constitutive surface expression of TLR2, TLR4 and TLR9 on human monocytes and alveolar macrophages and monocyte-derived macrophages. TLR levels were...

Ngày tải lên: 12/08/2014, 14:20

... receptor expression and B cell migrationChemokine receptor expression and B cell migration. Peripheral blood mononuclear cells were tested in a transmigration assay. A cytometric analy- sis showed ... S, Wu L, LaRosa G, Ponath P, Park CC, Pope RM, Koch AE: Differential expression of chemokine receptors on peripheral blood, synovial fluid, and synovial tissue monocytes...

Ngày tải lên: 09/08/2014, 06:23

... using antibody from R&D Systems confirmed the expression of this antigen mainly on endothelial cells. Tie2 mRNA and protein expression fol- lowed the same pattern of expression as Tie1. There ... DNA polymerase (Gibco/Life Technologies, Grand Island, NY, USA). The thermal cycling conditions included 94°C for five minutes, followed by 40 cycles of amplification at 94°C for...

Ngày tải lên: 09/08/2014, 03:24

... Rochester, NY, USA). Cytofluorometry The expression of RANK-L and RANK at the membrane was evaluated by cytofluorometry. Freshly separated healthy human blood or SF neutrophils from patients with RA and healthy ... not yet detectable on day 2 and appeared only after 3 days (Figure 3). The incubation of neutrophils in SM, however, strikingly upregulated the expres- sion of th...

Ngày tải lên: 09/08/2014, 10:20

... increases PLCγ enzymatic activity and tyrosine phosphorylation. FAK-induced PLCγ phosphorylation may be due to FAK interaction and activation of Src family kinases [6]. Our results demonstrate that ... h). pSrc localization Integrin αvβ3 activation induces FAK and Pyk2 phosphoryla- tion via Src. Phosphorylation of FAK and Pyk2 results in forma- tion of a signaling complex consis...

Ngày tải lên: 09/08/2014, 10:21

... Redaelli A, Roffi L, Antonelli L, Pistello M, Dianzani F: Correlation of interferon-induced expression of MxA mRNA in peripheral blood mononuclear cells with the response of patients with chronic active ... of viral genes and of cellular genes by repressing their expression. Second, cellular miRNAs may recognize viral RNAs and silence them, or control the expression of...

Ngày tải lên: 12/08/2014, 02:20

... accumulation of pigmented granules (probably copper) in hepatocytes, and inflammation with lymphocytes and pigmented (probably copper) macrophages. HE staining. (B) Centrolobular accumulation of copper ... reduction of GSS mRNA levels Western blot analysis of the metallothionein proteinsFigure 5 Western blot analysis of the metallothionein proteins. Immunoreactive bands of...

Ngày tải lên: 13/08/2014, 13:20

... treatment of methylcellulose on expression of PV L1 genes in mouse primary KCsFigure 2 Effects of post-transfection treatment of methylcellulose on expression of PV L1 genes in mouse primary KCs. ... pre-transfection treatment of methylcellulose affected transcriptional and translational expressions of the four PV L1 expression constructs in transiently L1-transfecte...

Ngày tải lên: 18/06/2014, 18:20

... treatment of methylcellulose on expression of PV L1 genes in mouse primary KCsFigure 2 Effects of post-transfection treatment of methylcellulose on expression of PV L1 genes in mouse primary KCs. ... dif- ferentially regulate expression of papillomavirus authentic and codon modified L1 genes. Virology 2007, 365:187-197. 10. Watt FM, Jones PH: Expression and functio...

Ngày tải lên: 20/06/2014, 01:20

... known as stromelysin-2) is a member of the stromelysin family of enzymes, along with MMP-3 (stromelysin- 1) and MMP-11 (stromelysin-3). This family of enzymes exhibit a wide range of substrate specificities, ... activation of a number of other members of the MMP family in a range of cell types, including articular chondrocytes [19,20,32]. The activation of MMP by other mem...

Ngày tải lên: 09/08/2014, 14:22