Báo cáo y học: " PDGF-Rα gene expression predicts proliferation, but PDGF-A suppresses transdifferentiation of neonatal mouse lung myofibroblasts" ppt

... harvested by trypsinization followed by gentle scraping and centrifugation. Preparation of freshly isolated mouse lung fibroblasts for Flow Cytometry analysis of α SMA and Ki67 Freshly isolated mouse ... of PDGF-Rα expression in mouse lung fibroblasts changes during alveolar development. Mouse lung fibroblasts were isolated from either wildtype (WT, A) or mice carry...

Ngày tải lên: 12/08/2014, 14:20

... properly cited. Array analysis of cycloxygenase-deficient mice<p>Microarray analysis of gene expression in the cerebral cortex and hippocampus of mice deficient in cyclooxygenase-1 or cyclooxygen-ase-2 ... increased expression of three genes that work in tandem in the final steps of short chain lipid metabolism by β oxidation, namely hydroxyacyl-coenzyme A dehydrogenase...

Ngày tải lên: 14/08/2014, 17:22

... Different factors and a variety of genes may contribute to the chain of events that eventually lead to lymphomagenesis. In an initial study to identify genes differentially transcribed in HIV and ... h2, respectively). The latter genes may represent new genes associated with lymphomagenesis but undetectable by microarray. Differences in expression of genes of the second gr...

Ngày tải lên: 02/11/2012, 11:08

... analyzed individually. Flow cytometry analysis The cell type composition and level of protein expression for some genes in the inguinal lymph nodes were analyzed by flow cytometry. Single cell suspensions ... of cadherins expressed by murine thymo- cytes. Cell Immunol 1996, 169:309-312. 38. Ohtsuka T, Ryu H, Minamishima YA, Ryo A, Lee SW: Modulation of p53 and p73 levels by cyclin G:...

Ngày tải lên: 09/08/2014, 01:23

... is hypothesised to be due to increased OB biosynthetic activity [18]. The lack of differential gene expression of these anabolic growth factors in our study is most likely due to the gene expression ... [11]. ALP is used as an enzymatic marker of bone formation, expressed by early-differentiated OB cells. Elevated expression of this enzyme in OA, as indicated by this study a...

Ngày tải lên: 09/08/2014, 08:23

... consistency between the microarray and PCR detection of expression ratio differences for each of the genes analyzed, suggesting that the majority of the genes identified by the microarray are bona ... human primary subchondral osteoblasts [39]. In the present study, we used microarray analysis to survey comprehensively the expression levels of many thousands of genes simultaneou...

Ngày tải lên: 09/08/2014, 10:21

... classification of the genes was performed using gene ontology and pathway analysis. Quantitative RT-PCR analysis of host genes using SYBR Green I The differential expression data was validated by quanti- tative ... Carlsbad, CA, USA) and purified by the RNeasy kit (Qiagen, Germany) following standard methodology. Amplification of RNA and indirect labeling of Cy-dye was done by Ami...

Ngày tải lên: 12/08/2014, 01:21

... biology of pulmonary angiogenesis. Post-pneumonectomy compensatory lung growth is a particularly intriguing example of pulmonary angiogen- esis. Within weeks of pneumonectomy, compensatory lung ... phases of the cell cycle. To investigate the endothelial response to pneu- monectomy, the lung digests were analyzed by flow cytometry. Baseline analysis by flow cytometry demon- stra...

Ngày tải lên: 12/08/2014, 13:22

... histology of generalized pulmonary emphy- sema. I. The genesis of the early centrolobular lesion: focal emphysema. Australas Ann Med 1957, 6:124-140. 8. McLean KH, Gough J: The histology of generalized ... of ECM gene expression. TGFβ, TNF, and CSE cause differential downregulation of decorin gene expression in COPD patients, at least partially via the Smad pathway. Our findi...

Ngày tải lên: 12/08/2014, 14:20

... global gene expression patterns in the lung were analyzed independ- ently at each of the four time points following pneumon- ectomy (6 hours, 1 day, 3 day and 7 day). In this time- dependent analysis, ... genes) from mouse lung tissues following unilat- eral pneumonectomy using Affymetrix GeneChip micro- array technology. Samples were taken at four time points (6 hours, 1 day, 3 d...

Ngày tải lên: 12/08/2014, 14:20