Báo cáo y học: " Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells" pps

... Kim DY, Ryu SY, Lim KE, Lee YS, Ro JY: Anti-inflammatory mechanism of simvastatin in mouse allergic asthma model. Eur J Pharmacol 2007, 557:76-86. 32. Kim JY, Kim DY, Ro JY: Granule formation in ... cell tryptase and Smad3 protein in lung tissues In order t o investigate whether smoke- induced expres- sion of Smad proteins was occurring in mast cells, we examined co-lo...

Ngày tải lên: 12/08/2014, 13:22

... inflammatory response in the lungs of mice which is linked to a reduction in HDAC activity and may be a key mechanism of glucocorticoid insensitivity in COPD [11,17,21]. Both this reduction in HDAC ... plethora of pathways both directly and in directly. Therefore it is unlikely that a change in a single proteins activity, expression or function would be solely responsi- ble for...

Ngày tải lên: 11/08/2014, 03:20

... machinery including the core histone proteins resulting in their in re-association with the DNA, thus presenting a transcriptionally closed con- formation [1,16]. HDAC-2 function is impaired by ... iden- tified [2,6]. However, two of the key components include histone deacetylases 1 and 2 (HDAC1/2) and methyl transferases (including methyl-CpG-binding proteins) * Correspondence: john....

Ngày tải lên: 11/08/2014, 06:22

... filter)(Uni- versity of Kentucky, Lexington, KY, USA) for 7 min, 4 times a day. Carboxyhemoglobin-levels in blood were 8.29 ± 1.4% in smoke- exposed vs. 1.02 ± 0.22% in air- exposed mice (n = 7). During air or smoke ... Appl Physiol 1994, 77:1011-1014. 19. De Swert KO, Tournoy KG, Joos GF, Pauwels RA: The role of the tachykinin NK1 receptor in airway changes in a mouse model of...

Ngày tải lên: 12/08/2014, 16:20

... blotting, biotinylation and immunoprecipitations Cell lysates were prepared by pre-treating cells with 10 mM N- ethyl maleimide (NEM) in PBS on ice for 10 minutess, and then lysed in 1% NP40 lysis ... formaldehyde in PBS, blocked with 1% BSA in PFN containing 0.2% saponin, stained with antibodies in PFN with 0.2% saponin, and stained with FITC-labelled sec- ond stage. The Golgi mar...

Ngày tải lên: 09/08/2014, 13:21

... Open Access Inhibitory effect of small interfering RNA on dengue virus replication in mosquito cells Xinwei Wu 1,2† , Hua Hong 3† , Jinya Yue 1,4 , Yejian Wu 1 , Xiangzhong Li 1 , Liyun Jiang 1 , ... (DHF) and dengue shock syndrome (DSS) [1,2]. They transmit diseases to human beings primarily through mosquitoes, mainly Aedes aegypti and Aedes albopictus. With drama- tically growth in re...

Ngày tải lên: 12/08/2014, 01:22

... that ROS play an important role in CSM-induced TLR4 associated intracel- lular signaling. Interestingly, we have found that CSM spe- cifically induced phosphorylation of IRAK-1 in stably transfected ... and therefore protecting the cells against oxidants (indirect effect) by modulating the redox signaling pathways [22,23]. Thus these results indicate that CSM by inducing ROS generation, m...

Ngày tải lên: 11/08/2014, 08:22

... receptor 2 or kinase insert domain receptor (a type III receptor tyrosine kinase) or protein-tyrosine kinase receptor FLk-1 (VEGFR2) is key in endothelial survival and th e mainte- nance of the ... IL-3 can inhibit the apoptotic activity of Bad by activating intra- cellular signaling pathways that results in phosphoryla- tion of Bad (Ser112 and Ser136) [22]. This further leads to binding...

Ngày tải lên: 11/08/2014, 08:22

... manually-controlled cigarette smoking machine that produces a combination of side- stream and mainstream cigarette smoke in a chamber, which is then transported to a collecting and mixing chamber where varying ... parallel induc- tion of the osteopontin and CD207 genes occurs in smok- ers with COPD, and suggests the possibility that cigarette smoke- induced osteopontin levels in...

Ngày tải lên: 12/08/2014, 11:21

... assayed by zymography, and net gelatinase activity in BALF assayed by digestion of fluorescein cou- pled gelatin, as previously described [12]. Densitometry of zymography gels was done using ... purposes) Flow cytometry quantitation of T lymphocytesFigure 6 Flow cytometry quantitation of T lymphocytes. CD3+ CD4+ lymphocytes in (a) BALF & (b) LNs, and CD3+ CD8+ lym- phocytes in (c) B...

Ngày tải lên: 12/08/2014, 15:21