Báo cáo y học: " Comprehensive characterisation of pulmonary and serum surfactant protein D in COPD" docx

... performed from cytospin slides, with 300 cells per slide being counted. Protein content in BAL and serum was determined according to the method of Bradford [21]. Serum sampling Blood was collected in ... were loaded as indicated by equal band intensities of the SP -D monomer (43 kDa) in SDS page under reduced conditions and immunoblotting (upper panel A and B). The quat...

Ngày tải lên: 12/08/2014, 13:22

... autoradiography and correspond to R f values of unlabeled nucleotides visualized under UV light [23]. Radiolabeling of AAC proteins synthesized in E. coli and enrichment of the histidine-tagged chimeric ... mitochondria, we added glycine, malate, pyruvate and NAD + to the media used for isolation and storage [39,40]. To analyse uptake of radioactively labeled nucleotides int...

Ngày tải lên: 18/03/2014, 01:20

... role played by this protein during the virus life cycle, as such amphi- pathic helices are believed to be involved in protein lipid bilayers, protein protein and/ or protein nucleic acid inter- actions. Keywords:Vpr;NMR;HIV-1;helix;3Dstructure. The ... well-defined hydrophilic and hydrophobic faces, is commonly observed in structural motifs that involve protein protein, protein...

Ngày tải lên: 31/03/2014, 23:20

... helix-loop-helix and Per-Arnt-Sim domain creates a functional interface for dimerization of HIF-α with HIF-β and binding to HRE. The PHD enzymes hydroxy- late proline residues in the oxygen-dependent degradation domain, ... and PHD-3, and to a lesser extent PHD-1, are strongly induced by hypoxia in many cell types, thus resulting in the increased oxygen-mediated HIF-α degradat...

Ngày tải lên: 09/08/2014, 01:22

... largely unknown how these enzymes are regulated. PHD-2 and PHD-3, and to a lesser extent PHD-1, are strongly induced by hypoxia in many cell types, thus resulting in the increased oxygen-mediated ... of disease. In RA synovium, HIF-1α and HIF-2α are expressed in the synovial lining and stromal cells [75]. In adjuvant-induced arthritis, HIF-1α has been localized to synovium...

Ngày tải lên: 09/08/2014, 13:22

... participated in the design of the study. CB participated in the design of the study and performed statistical analysis. GC partici- pated in the design of the study and helped with its coor- dination. ... food-entrainable oscillator and the existence of a daily rhythm of serum urea concentration influenced by feeding in ruminants led us to investigate the rhythmic pa...

Ngày tải lên: 10/08/2014, 09:20

... resolved by discussion and involvement of another reviewer where necessary. Methods for synthesising findings Findings will be reported using consensual guidelines for narrative syntheses and meta-analytical ... spite of these initia- tives, significant gaps remain in understanding the chal- lenges related to developing, implementing, and maintaining health information systems. Un...

Ngày tải lên: 11/08/2014, 05:21

... (Shino-test, Tokyo, Japan). In the same series, a portion of the liver was excised and the specimens were fixed in 10% buffered formalin, embedded in paraffin, stained with H&E, and examined ... manuscript. EN and TT processed the data. KN, TS and YO performed the experiment and collected the data. All authors read and approved the final manuscript. Acknowledgements A...

Ngày tải lên: 13/08/2014, 20:21

... collected infor- mation on body measurements (including height and weight), medication use (including diuretics, anti-hypertensives, allopu- rinol and uricosuric agents), medical conditions (including ... 1.3 Sweetened soft drink intake (servings/day) 0.3 0.2 0.2 Coffee intake (servings/day) 1.2 1.2 1.3 Uric acid drug use a (%) 1.4 0.4 1.4 Creatinine (mg/dl) 1.0 1.0 1.0 Data are presente...

Ngày tải lên: 09/08/2014, 13:22

... haematoxylin, dehydrated through the as- cending graded alcohols, cleared in xylol and finally coverslipped using Entellan. 3. RESULTS Pathological findings The results of laboratory investigations ... dissociated upon reduction of disulphide bonds with DTT. The colonic and respiratory epithelia predominantly expressed acidic mucin of the sialated and sulphated types res...

Ngày tải lên: 26/10/2012, 10:03