Báo cáo y học: " Phenotypic characteristics of human type II alveolar epithelial cells suitable for antigen presentation to T lymphocytes" pptx

... flow cytometry (see below). Flow cytometry This technique was used to assess the purity of the cell suspensions and to characterize their phenotype after cytokine stimulation. Cells were first incubated ... on cytokine-activated cells. To mimic inflammation, we chose to analyze the effect on the AECII phenotype of two major inflammatory cyto- kines, IFN-g and TNF-a, known to...

Ngày tải lên: 12/08/2014, 13:22

... suggests that this cell type may be another critical target for the treatment of inflammatory arthritis [11]. Mesenchymal stem cells (MSCs) are cells of stromal origin that can exert profound ... immu- nohistochemistry in the joints of UC-MSC-treated mice, suggesting that injected UC-MSCs did not restore tissue integrity by mechanisms of tissue repair (data not shown).However,...

Ngày tải lên: 12/08/2014, 15:22

... sur- rounding tissues [4]. Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into a variety of cell types. In the field of cell transplantation, MSCs have many advantages ... mi- gration of MSCs were dynamically monitored to evaluate the feasibility and safety of intravenous im- plantation of allogeneic MSCs in the treatment of femoral head...

Ngày tải lên: 25/10/2012, 11:18

... especially with respect to its glycone/aglycone speci®city a nd ability to catalyse the hydrolysis of dietary xenobiotic glycosides. This is also the ®rst report describing heterologous expression of a ... b- D -arabino- sides), similar to the native enzyme. For the ®rst time, we show that the human enzyme has signi®cant activity towards many common dietary xenobiotics including...

Ngày tải lên: 08/03/2014, 16:20

... topoisomerase T7 22A mutation that increases the stability of Topo I– DNA covalent complex, thus mimicking the cytotoxic effect of camptothecin [9], has also rendered low viability of the yeast mutant carrying ... treating cancers that are refractory to camptothecin treatment. In order to understand the structure–activity relationship of TDP and to facilitate inhibitor screenin...

Ngày tải lên: 31/03/2014, 21:21

... modified myoepithelial cells of the stromal tissues. Furthermore, the cyto-proliferation of these modified myoepithelial cells continued to the bone forming cells, which reacted to Runx2, ... well-known osteoblastic maker. Therefore, the results strongly suggest that the bone forming cells (osteoblasts and osteocytes) originated from the modified (neoplastic) myoepitheli...

Ngày tải lên: 08/08/2014, 16:23

... dependent and were not neutralized by anti-TGF-β or IL-10. Adding less than 1% of these cells to T cells strongly inhibited the generation of cytotoxic T- lymphocyte activity by preventing the activation ... extremely potent suppressive activity. These CD4 + cells had the phenotype and functional characteris- tics of ‘professional’ regulatory T cells. Using the genera- tio...

Ngày tải lên: 09/08/2014, 03:24

... the type of Th cell that stimulates them. This is consistent with the capacity of fibroblasts to activate flexible patterns of cytokine production readily. A corollary of this observation is the ... NP40 to detect cytokine content. Statistical analysis Student's t test was used with two-tailed P values or Mann– Whitney U test using StatView 5.0™ (SAS Institute Inc., Cary,...

Ngày tải lên: 09/08/2014, 07:20

... might also contribute to the pathophy- siology of the disease. To evaluate this possibility, we conducted an experiment to investigate the expression levels of wild -type and mutant proteins in the ... cytosolic (Cy) and mitochondrial (Mi) extracts from T- REx-293 cells transiently transfected with the wild -type or NDUFV2 pathogenic mutant construct. (c) The expression levels...

Ngày tải lên: 10/08/2014, 05:21

... of immunity display an extraordinary capacity to induce T cell responses and secrete a variety of cytokines; the differentiation status of DCs can influence whether its target lymphocyte–often T cells will ... potent antigen- presenting cells (APCs) that act by internalizing, shut- tling, and presenting antigens to naïve T -cells, which then leads to T- cell a ctivatio...

Ngày tải lên: 10/08/2014, 05:21