Báo cáo y học: " A novel small molecule target in human airway smooth muscle for potential treatment of obstructive lung diseases: a staged highthroughput biophysical screening" pps

... RESEARC H Open Access A novel small molecule target in human airway smooth muscle for potential treatment of obstructive lung diseases: a staged high- throughput biophysical screening Steven S An 1*† , ... single-cell biophysical measurements. KA performed statistical analysis; KA and SSA analyzed the data. JJF and SS oversaw the project. SSA wrote th...

Ngày tải lên: 12/08/2014, 13:22

... auto- matically calculated with Bio-Plex Manager software by using a standard curve derived from a recombinant cytokine stand- ard. According to previous experiments analyzing 17 cytokines (IL-1, ... allowing the detection of cytokines without any BALF manipulation that could influence the stability of cytokines. By using this technique, we observed abnormalities in a broad range...

Ngày tải lên: 09/08/2014, 14:22

... Canada. 3 CIHR IMPACT Training Program in Pulmonary and Cardiovascular Research, University of Manitoba, Winnipeg, MB, Canada. 4 CIHR National Training Program in Allergy and Asthma, University of Manitoba, Winnipeg, ... that targeting regulators of isoprenoid-dependent signaling holds promise for treating airway wall fibrosis. Keywords: airway fibroblasts, airway remodeling...

Ngày tải lên: 12/08/2014, 14:20

... major basic protein analogue poly-L-arginine inhibits L-arginine uptake [37] and causes airway hyperreactivity to metha- choline in perfused guinea pig tracheal preparations by causing a deficiency ... particular importance in relation to reduced bioavailability of L-arginine in the air- ways is increased activity of arginase, which hydrolyzes L- arginine into L-ornithine and ur...

Ngày tải lên: 12/08/2014, 16:20

... follow: Pharmacological activation of CFTR chloride channels activity in rat airway smooth muscle cellsFigure 3 Pharmacological activation of CFTR chloride channels activity in rat airway smooth muscle ... transports in rat airway smooth muscle cells. A Immunofluorescence study of α -smooth muscle actin in tracheal smooth muscle cells from rat and B...

Ngày tải lên: 12/08/2014, 16:20

... may act as a paracrine factor for cell body maintenance and probably influence axonal regeneration as it has been described for certain types of central neurons[61]. The present study, using a ... Chadi G: Glial bFGF and S100 immunoreactivities increase in ascending dopamine pathways following striatal 6-OHDA-induced partial lesion of the nigrostriatal system: a sterological a...

Ngày tải lên: 10/08/2014, 10:20

... directly examined in airway epithelial cells of small airway origin. We hypothesized that primary human small airway epi- thelial cells (SAECs) would produce detectable NO gas phase release following ... breath of healthy subjects. However, asthma is thought to be an inflammatory dis- ease of the smaller airways. The phenotypic features of the airway epithelium change...

Ngày tải lên: 12/08/2014, 14:20

... 5'-TGAGGAGCAGCACCCAGAGC-3' Antisense 5'-CCGTAGGACTGGAAAGAGGA-3' Human TNFα Sense 5'-GTCTCCTACCAGACCAAG-3' Antisense 5'-CAAAGTAGACCTGCCCAGACTC-3' Human β-actin Sense 5'-TTCCTGGGCATGGAGTCCT-3' Antisense ... 5'-TTCCTGGGCATGGAGTCCT-3' Antisense 5'-AGGAGGAGCAATGATCTTGATC-3' Mouse TNFα Sense 5'-CATCTTCTCAAAATTCGAG-3&apos...

Ngày tải lên: 09/08/2014, 08:23

... will hamper a clear adjudication of the reason for withdrawal. In summary, the study by Saad and colleagues gives us important insights into daily-life use of TNF inhibitors in PsA, a disease that too often ... this paradox. In RA, therapy steered by an index has become more and more a mainstay of management as it has been shown that it is not the level of improvement th...

Ngày tải lên: 09/08/2014, 14:20

... H, Matsumoto K, Tsuda M, Fukuyama S, Koto H, Aizawa H, Kureishi Y, Hara N, Nakanishi Y: Activation of PI3K-Akt pathway mediates antiapoptotic effects of b-adrenergic agonist in airway eosinophils. ... patterns have been reported in many human diseases, particularly cancer, and investiga- tors have used HDAC inhibitors against many malignan- cies. HDAC inhibitors induce apoptotic...

Ngày tải lên: 11/08/2014, 08:22