Báo cáo y học: "Phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus" pot

... is properly cited.Research articlePhenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosusYing J Nie1, Mo Y Mok1, Godfrey CF Chan2, ... BMDCs, bone marrow-derived dendritic cells; BMCs, bone marrow cells; FLDCs, dendritic cells induced by FL; mDCs, myeloid dendritic cells; FL, FMS-like tyrosine kinase 3 ligand; GM-CSF, granulocyte ... SLE, systemic lupus erythematosus; FLDCs, dendritic cells induced by FL; mDCs, myeloid dendritic cells induced by GM-CSF + IL-4; FL, FMS-like tyrosine kinase 3 ligand; GM-CSF, granulocyte macrophage-col-ony-stimulating...

Báo cáo y học: "Phenotypic and functional characterisation of CD4+ memory T cells homing to the joints in juvenile idiopathic arthritis" pps

... 2c–e). In the former, CCR7 expression was detected mostly in theperivascular lymphocytic infiltrates of the sublining layer(Fig. 2b,o) and, only occasionally, in scattered cells in thesublining ... at the level of lymphocyte aggre-gates (Fig. 2e,f).Conversely, CCR5-positive cells were detected mainly in the lining layer and in the sublining zone of the superficialsubintima and, to a smaller ... expressed in synovial tissue, with aclear distribution both in lymphoid aggregates and in perivascular infiltrates of sublining layer and in the lininglayer.Conversely, CCR7-positive and CCR5-positive...

Báo cáo y học: "Phenotypic and functional characterization of switch memory B cells from patients with oligoarticular juvenile idiopathic arthritis" pps

... A: Bone marrow-derived mesenchymal stem cells induce both polyclonal expansion and differentiation of B cells isolated from healthy donors and systemic lupus erythemato-sus patients. Stem Cells ... Chemokine and chemokinereceptor analysis reveals elevated interferon-inducible pro-tein-10 (IP)-10/CXCL10 levels and increased number of CCR5+ and CXCR3+ CD4 T cells in synovial fluid of patientswith ... numbers of plasma cells [37].Accordingly, we found an enrichment for IgG-secretingplasma blasts in SF and a large amount of IgG-producing B cells in synovial tissue. The functional significance of...

Báo cáo y học: "Transcriptional and structural impact of TATA-initiation site spacing in mammalian core promoters" potx

... are depleted of PyPu dinucleotides immediately upstream of the dominant TSS [-1,+1] (namely, [-2,-1] and [-3,-2]; fraction of PyPu dinucleotides shown as grey bars) and have a PyPu consensus ... spacing influences initiation site usageFigure 4TATA-TSS spacing influences initiation site usage. Histogram showing the distribution of the four possible dinucleotides (PyPu, PyPy, PuPy, and ... occurrence of PyPu dinucleotidesimmediately upstream and downstream of the TSS [-1, +1] in the different TATA-TSS spacing classes (-34 to -28). Using a2 bp sliding window, we counted the PyPu dinucleotides...

Báo cáo Y học: Structural and functional characterization of a C-type lectin-like antifreeze protein from rainbow smelt (Osmerus mordax) potx

... trypsin, untreated AFP (UT AFP), and deglycosylated AFP (DG) are id entiﬁed.Bands smaller than 1 4 kDa in lan es containing trypsin were shown to b e trypsin autolysis products (trypsin-only ... [19].Dimerization of smelt AFP is unlikely to increase activityby increased ice binding because only a dimer with perfectlyaligned ice-binding sites would have enhanced binding to ice and this is not likely ... of a Ca2+-bindingsite. Further evidence for direct Ca2+binding by smelt AFPwas obtained using intrinsic ﬂuorescence. An increase in intrinsic ﬂuorescence emission intensity was shown to...

Báo cáo khoa học: "Phenotypic and functional analysis of bovine γδ lymphocytes" pps

... of cytokines in activation and function of γδγδγδγδ T cells activated indirectly through cytokinessecreted by ab T cells, accessory cells and antigenpresenting cells (APC). Cytokines were ... removal of γδ T cells early in cultures leadsto greater proliferation of CD4+ cells and facilitates cloningPhenotypic and functional analysis of bovine γδ lymphocytes 41with anti-δ and ... obtained on the capacity of γδ T cells toproduce cytokines.To fully delineate the regulatory and effector activities of γδ T cells in ruminants, it will be essential to detail thecapacity of...

Báo cáo y học: "Expression and functional properties of antibodies to tissue inhibitors of metalloproteinases (TIMPs) in rheumatoid arthritis" docx

... certain inflammatory cytokines (IL-6, IL-1β) and by certain growth factors.Extracellularly, TIMPs inhibit MMP activity by forming high-affinity noncovalent complexes with MMPs. The amino-termi-nal ... amino-termi-nal domain of TIMP binds the active site of MMPs, inhibitingtheir proteolytic activity. The carboxy-terminal domain of cer-tain TIMPs has also the ability to form complexes with proen-zymes (proMMPs) ... antibodies. Lysates of THP-1 (a human monocytic cell line) and H9 (a human T-cell lymphoma) were separated in 18% Tris-glycine gel, transferred into a polyvinylidene fluo-ride membrane, and blotted...

Báo cáo y học: "Structural and functional map of a bacterial nucleoid" doc

... orders of magnitude to fit into the space available [1,2], and the mechanism of chromo-somal packing in bacteria and the proteins involved is a long-standing question. In a recent study published ... analyzed and understood together. Nucleoid topology is strongly related to the binding patterns of NAPs. All the major NAPs, with the exception of Dps (the DNA-binding protein in starved cells) , ... 1). They then determined the transcrip-tional profiles of the EPODs by DNA microarray analysis and found that they fell into two groups - highly expressed (heEPODs) and transcriptionally silent...

Báo cáo y học: "Structural and functional characterization of human apolipoprotein E 72-166 peptides in both aqueous and lipid environments" pot

... positive ly correlates with the increasingconcentration of DHPC.Protein-lipid interactions and Protein-LDLR binding of ApoE-(72-166) ProteinsTo identify and compare the lipid binding ability of thethree ... three isoforms were significantly dissociated in the presence of dihexanoylphosphatidylcholine.Dimyristoylphosphatidylcholine turbidity clearance assay showed that apoE4-(72-166) maintains the ... lipid-binding capacity. Finally, only apoE4-(72-166) still maintained significant LDL receptor binding ability.Conclusions: Overall, apoE4-(72-166) peptides displayed a higher lipid-binding and...

Báo cáo y học: " Genetic and functional analysis of HIV-1 Rev Responsive Element (RRE) sequences from North-India" pdf

... splicing machinery very efficiently by using the Revprotein which binds with high affinity and specificity tohighly structured cis-acting RNA element present withinthe coding region of HIV-1 ... etic diversity and has beenclassified into various subtypes and recomb inant forms.While subtype B predominates in US and UK, it is sub -type C that is predominant in India, China and SouthAfrica. ... work is properly cited.known to interact with RRE structures and modulate thesplicing ability of Rev protein. In summary, we genetically characterized the nature of heterogeneity in the RRE sequences...

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