Báo cáo y học: "If good things come from above, do bad things come from below" ppt
... cysts. These data provoke questions about the nature and role of bone marrow lesions. © 2010 BioMed Central Ltd If good things come from above, do bad things come from below? David M Findlay* See ... lubricin, come from above. Is it possible that factors which lead to the destruction of cartilage come from below in the subchondral bone? The recent acquisition of...
Ngày tải lên: 12/08/2014, 12:20
... methodology to acknowledge cultural difference and build understanding. BMC Medical Research Methodology 2009, 9:60. 36. Braun V, Clarke V: Using thematic analysis in psychology. Qual Res Psychology ... worried taking any of that " Secrecy and mystery abound in the Aboriginal commu- nity about the use and availability of bush medicine. This inevitably means poor availability of accurat...
Ngày tải lên: 10/08/2014, 09:21
... former study of CAD patients from our institution [17], which may be explained by the fact that patients from this study were selected on the basis of high tHcy levels. A statistically-significant ... are required daily to treat hyperhomocysteinemia. To reach this amount by dietary changes alone may be difficult because food has a low folic acid content and bioavailability. No studies...
Ngày tải lên: 02/11/2012, 11:12
Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... correlate exactly with the mobilities of di-, mono- and unglycosylated forms of recombinant cystatin F previously characterized by enzymatic removal of N-linked carbohydrate and carbohydrate composition analysis ... composition analysis [5]. It is also noteworthy that all three forms were partially purified by their affinity to Cm-papain, strongly indicating that the carbohydrate side chains d...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: The Ikaros family protein Eos associates with C-terminal-binding protein corepressors pptx
... vector pGL2- TK (Promega). Yeast two-hybrid and pull-down assays The Clontech yeast two-hybrid system was used according to the manufacturer’s instructions. The prey and bait plasmids used are ... 2002 The Ikaros family protein Eos associates with C-terminal-binding protein corepressors Jose ´ Perdomo and Merlin Crossley Department of Biochemistry, G08, University of Sydney, NSW, Australia Eo...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... enzyme, a plausible explanation may be that 4-phosphorylated UTP by itself acts as a weak activator of glutamine hydrolysis. This activation is greatly enhanced by GTP binding to the enzyme. From ... glutamine hydrolysis has been shown to be greatly stimulated by bicarbonate- dependent ATP hydrolysis, indicating that for this enzyme the phosphorylated amino acceptor intermediate, carbonyl p...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx
... recombinant aA-crystallin and its two domains. Bi ochim. Biophys. Acta 1130, 267–276. 8. Augusteyn, R.C. (1998) a-Crystallin polymers and polymerization: the view from down under. Int. J. Biol. Macromol. ... variants produced by p26-ND60 and p26-alpha were pr esent but recognized poorly by antip26 antibody. To determine if recognition of p26-ND60 and p26-alpha encoded polypeptides by antibo...
Ngày tải lên: 22/02/2014, 04:20
Báo cáo Y học: Two different E2F6 proteins generated by alternative splicing and internal translation initiation ppt
... confirmed by FACS analysis. RNA isolation Total RNA was isolated from primary MEFs with the RNeasy kit (Qiagen) according to the manufacturer’s instructions. To isolate RNA from primary mouse tissues, an ... c-myc, which is mediated by an internal ribosome entry site during apoptosis [18], and efficient translation of vascular endothelial growth factor from an internal ribosome entry site...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot
... permethylated total N -glycans enzymatically liberated from huma n HCI I isolated (A) from human serum or ( B) produced by genetically engineered CHO cells. The following complex type carbohydrate ... permethylation of oligosaccharides The enzymatically liberated N-glycans were reduced and permethylated as described previously [27]. MALDI-TOF MS The reduced and carboxamidomethylated trypt...
Ngày tải lên: 08/03/2014, 22:20
Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx
... a lyase-isomeraseforthephotoactive3 1 -Cys-a84-phycoviolobilin chromophore of phycoerythrocyanin. Biochemistry 40, 12444– 12456. Ó FEBS 2002 Analyses of a-phycoerythrocyanin peptides (Eur. J. Biochem. 269) 5055 Optical spectroscopy The ... phycobiliprotein lyase: both the attachment of phy- cocyanobilin and the isomerization to phycoviolobilin are cata- lyzed by the proteins PecE and PecF enc...
Ngày tải lên: 17/03/2014, 10:20