Báo cáo y học: "Automated evaluation of autoantibodies on human epithelial-2 cells as an approach to standardize cell-based immunofluorescence tests" pdf

... Italian Society of Laboratory Medicine Study Group on the Diagnosis of Autoimmune Diseases: Guidelines for the laboratory use of autoantibody tests in the diagnosis and monitoring of autoimmune ... Burmester 1 Abstract Introduction: Analysis of autoantibodies (AAB) by indirect immunofluorescence (IIF) is a basic tool for the serological diagnosis of systemic rheumatic disord...

Ngày tải lên: 12/08/2014, 12:20

... pep- tide synthesis was purified by high-performance liquid chro- matography. The quality and purity of the peptide was assessed by mass spectrometry and analytical high-perform- ance liquid chromatography. ... between anti-PM/Scl anti- bodies and anti-Ro52. In contrast, Yamanishi and colleagues reported an association of PM/Scl syndrome with anti-Ku anti- body and rimmed vacuole format...

Ngày tải lên: 09/08/2014, 06:22

... patient's history and physical examination. If the suspicion of IgE-mediated food allergy is compelling based on the history, skin and serology tests are routinely performed to provide confirmation for ... J Allergy Clin Immunol 2008, 122:298-304. 51. Hamilton RG, Saito H: IgE antibody concentration, specific activity, clonality, and affinity measures from future diagnos- tic confi...

Ngày tải lên: 08/08/2014, 21:20

... manufacturer, human plasma was the source of CRP, which had been isolated by gel chromatog- raphy and had a purity of at least 99% as measured by SDS-PAGE. We also checked the CRP preparation by polymerase ... patients and those from controls on the microtitre plates were always randomly mixed and analysed at the same occasion. Inhibition assays Eleven patient sera with strong react...

Ngày tải lên: 09/08/2014, 01:23

... analysed factors. In this study, calculations are based on the combination of SE and the analysed autoan- tibodies. This is because anti-CCP antibodies and RFs are significantly associated, and ... associated, and consequently prediction of RA is only marginally increased when they are considered in combination. Conclusion This study has demonstrated that the presence of anti-CCP anti...

Ngày tải lên: 09/08/2014, 01:23

... prospectively analyzed serum chondroitin sulfate (CS) and hyaluronic acid (HA) levels using monoclonal antibody WF6 and enzyme-linked immuno- sorbent assay (ELISA), respectively, accompanying the ... immunoassay using monoclonal antibody WF6 A mouse monoclonal antibody WF6 was raised against a shark cartilage aggrecan preparation [8], and a quantitative ELISA for the epitope recognized by mo...

Ngày tải lên: 09/08/2014, 14:21

... Association. Authors’ contributions MD has made substantial contributions to conception, design, acquisition, analysis and interpretation. JPW has made substantial contributions to data acquisition. ... personal pre ference and skill of the implanting surgeon will continue to play an important role in choosing a certain valve type. Conclusion However, the overall complexity of pul...

Ngày tải lên: 10/08/2014, 09:22

... statis- tically analyzed using two-way analysis of variance (ANOVA). The data of tissue biomarker total calcium and MPO were analyzed using one way analysis of vari- ance (ANOVA). In both cases, Tukey's ... super- natant was used for estimating the total calcium levels by atomic emission spectroscopy at 556 nm. Estimation of myeloperoxidase activity MPO, an enzyme liberated due...

Ngày tải lên: 10/08/2014, 10:20

... Ultrasound imaging has previously been used on the foot to measure plantar fascia band thickness in symptomatic and asymp- tomatic feet and to establish a plantar fasica index [20]. Methodologically, previous ... palpated the bony anatomical land- mark of the anterior aspect of the medial malleolus and a perpendicular scanning line was drawn directly inferiorly. The ultrasound gel p...

Ngày tải lên: 10/08/2014, 21:23

... strains as belonging to genotype D, genotype A and mixed genotype A and D, respectively. On the contrary, the PCR-RFLP-based method correctly identified all 4 genotype A but only 56 of 63 genotype ... liver disease pro- gression, selection of mutants and response to antiviral therapy in acute and chronic HBV infections [3-5]. Con- sidering the importance of determining the HBV...

Ngày tải lên: 12/08/2014, 04:20