Báo cáo y học: "Functional consequences of DECTIN-1 early stop codon polymorphism Y238X in rheumatoid arthritis" pptx

... produce cytokines induced by dectin-1 signalling. Table 1 Genetic distribution of the DECTIN-1 Y2 38X polymorphism in a patient cohort of rheumatoid arthritis (n = 262) and in a group of healthy controls ... recently, the functional consequences of the Y2 38X early stop codon polymorphism in DECTIN-1 have been studied in detail. This polymorphism was de...

Ngày tải lên: 12/08/2014, 11:23

... have been reported in only one fifth of RA patients with early disease by immunoblot- ting [13,15], thus indicating a superior performance of the anti-MCV ELISA in this early- disease group compared ... usually not in a citrullinated state, but deimination of this protein occurs in macrophages undergoing apoptosis. Anti-citrullinated vimentin antibodies may then emerge as a c...

Ngày tải lên: 09/08/2014, 08:22

... occurs at the β-carbon of the asparagine residue, consequently (by way of steric hindrance) preventing the interaction of the HIF-α C-TAD with the cysteine/histidine-rich 1 domain of p300, a co-activator ... variation in the relative expression of the PHD isoforms in different cells, with PHD-2 being the most abundant HIF prolyl hydroxylase. Specific ‘silencing’ of all three e...

Ngày tải lên: 09/08/2014, 01:22

... being the most abundant HIF prolyl hydroxylase. Specific ‘silencing’ of all three enzymes using short interfering RNA has shown that PHD-2 is the major player in stabilizing HIF in normoxia in ... LxxLAP consensus motif for hydroxylation by PHD, thus linking two major human signalling systems, namely NF-κB and HIF. Mimicking hypoxia by treatment of cells with small interfering RNA a...

Ngày tải lên: 09/08/2014, 13:22

... recombinant protein containing the C-myc epitope followed by six histidines at the C-terminus. A plasmid containing the wild type version of CCR5 was constructed by introducing the coding sequence ... the C-terminus of CCR5 would interfere with the protein’s ability to function as a chemokine receptor or HIV coreceptor, we first determined the binding of MIP-1a to the CCR5-myc-his expres...

Ngày tải lên: 08/03/2014, 09:20

... expression of a variety of single mitochondrial-type AACs in E. coli. After IPTG-induction we were able to obtain functional integration of the various mitochondrial-type AACs into the cytoplasmic ... trans- ferredtonewreactionvesselsandheatedto95°Cfor 10 min to prevent any further reaction. An aliquot of the exported radioactive solution was used for separation by thin-layer chroma...

Ngày tải lên: 18/03/2014, 01:20

... residues phenylalanine and tyrosine typical of the Ig-fold are bold in each domain. Fig. 5. Inhibition of in vivo myotoxicity of myotoxins I or II by DM64. Groups of four mice were injected intramuscularly ... DM64 binds to the myotoxins through a myotoxic/ cytolytic site distinct from the catalytic site, as already described for the inhibition of myotoxicity by heparin [44]. At...

Ngày tải lên: 21/02/2014, 01:21

... Mchaourab, H.S. (1999) Folding pattern of the a-crystallin domain in aA-crystallin determined by site-directed spin labelling. J. Mol. B iol. 294, 561–577. 68. To ¨ ro ¨ k, Z., Goloubinoff, P., Horva ´ th, ... termed the a-crystallin domain, distinguishes a ll small heat shock/a-crystallin proteins, and a two or three domain structure is proposed for these proteins [7,8]. The a-crystallin...

Ngày tải lên: 22/02/2014, 04:20

... (naringin) Hesperetin-7-GlcRha (hesperidin) Dihydrochalcones Phloretin-7-Glc (phlorizin) Secoiridoids Oleuropein Anthocyanidins Cyanidin-3-Glc (kuromanin) Cyanidin-3,5-diGlc Fig. 4. Structure s of ... 4NP-b- D - glucopyranoside (10 m M ) was reduced by 98% in the presence of 4NP-b- D -mannopyranoside (10 m M )]. Hydrolysis of xenobiotic glycosides by reCBG The a bility of CBG to hyd...

Ngày tải lên: 08/03/2014, 16:20

... W101 and W162 are involved in the b inding of the UDP-galactose donor, t he residue W315 in the binding of the N-acetylglucosamine-b-p- nitrophenol acceptor, and the domain including E264 appears ... b1,3-N-acetylglucosaminyltransferases, thereby suggesting that they are more likely involved in mediating the b1,3-linkage speci®city r ather than in t he direct binding of the subs...

Ngày tải lên: 08/03/2014, 16:20