Báo cáo y học: "Identity of mysterious CD4+CD25–Foxp3+ cells in systemic lupus erythematosus" pps

... is likely that not all Foxp3 + T cells in SLE have protective suppressive activity. © 2010 BioMed Central Ltd Identity of mysterious CD4 + CD25 – Foxp3 + cells in systemic lupus erythematosus David ... of CD4 + CD25 + Foxp3 + regulatory T cells (Tregs) probably contribute to T- and B-cell hyperactivity in systemic lupus erythematosus (SLE), but reports on numbe...

Ngày tải lên: 12/08/2014, 11:22

... sera. We examined whether the binding of DNA to RPA can inter- fere the reactivity of anti-RPA autoantibodies by incubating affinity-purified RPA with ssDNA, dsDNA, or dsRNA before antibody binding. ... effi- ciently in high NaCl, and/or that the dissociation of interacting proteins and DNA by high NaCl and possibly secondary con- formational changes help the binding of anti-RPA ant...

Ngày tải lên: 09/08/2014, 08:22

... amplification in a 50-µl reaction mixture con- taining 1 U of DynaZyme II DNA polymerase (Finnzymes OY, Espoo, Finland), 5 µl of 10 × PCR Buffer (Finnzymes OY), 0.2 mM of each dNTP, and 15 pmol/µl of each ... determinations. Cells were harvested and the expression of cell-surface markers related to myeloid DCs was analyzed using flow cytometry. Measurement of cytokine levels C...

Ngày tải lên: 09/08/2014, 06:22

... processing in popula- tions of mature murine dendritic cells is caused by subsets of incompletely matured cells. J Immunol 1995, 155:93-99. 13. Chain B, Kay P, Feldmann M: The cellular pathway of ... hand, inhibitors of serine proteases (dichloroisocoumarin, DCI) or of cystein proteases (E-64) did not impair XS52 DC presentation of PPD, nor did they inhibit cathepsin D/E acti...

Ngày tải lên: 11/08/2014, 10:23

... disturbing cortical actin dynamics inhibited virus production [Fig. 3A]. This was observed either by modifying the polymerising actin itself, by Cyto D action, or by inhibiting one key GTP binding ... production. Inhibition of small GTP-binding proteins reduces infectivity of HIV-1 particles We further investigated whether toxin treatments of HIV- 1 producing cells had any effect on t...

Ngày tải lên: 13/08/2014, 09:21

... that models of lupus in mice deleted of both TLR7 and TLR9 with provide additional insight into the potential therapeutic role of TLR9 inhibition in lupus. Targeting type I interferons in systemic lupus erythematosus The ... and IL-10. In addition, Gas-6 on apoptotic cells binding to receptor kinases of Tyro 3 family (mer, tyro and axl) down- regulate inflammatory cy...

Ngày tải lên: 09/08/2014, 10:21

... activity by induction of suppressive Th2 cell activ- ity in RA therefore do not seem to be hampered by an intrinsic defect of naive T cells to respond to IL-4-inducing stimuli. Competing interests None ... are shown in (b). Cytokine production of the CD3/CD28 costimulated CD4 + T cells was assessed after 10 days of culture in the absence or presence of IL-7, of IL-4 or...

Ngày tải lên: 09/08/2014, 01:23

... endonucleases con- structed by covalently linking the DNA-binding domains of an array of 3-6 zinc fingers on to the non-specific DNA cleavage domain (or simply F N ) of the Flavobacterium okeanokoite ... but many z inc fingers can be combined to gen erate an array capable, in the case of our listed ZFAs of three fingers, of recogniz- ing nine nucleotides [54-57]. Owing to this u...

Ngày tải lên: 13/08/2014, 16:20

... Efficacy and validity of radiofrequency neurotomy for chronic lumbar zygapophysial joint pain. Spine (Phila Pa 1976). 2000; 25: 1270-7. 32. Merskey H, Bogduk N. Lumbar zygapophysial joint pain. In: ... Classification of Chronic Pain; Descriptions of Chronic Pain Syndromes and Definition of Pain Terms, 2 nd ed; Task Force on Taxonomy of the International Association for the S...

Ngày tải lên: 26/10/2012, 09:07

... The inflammatory cytokines, TNF-alpha and cer- tain interleukins play a key-role in initiating systemic inflammatory response syndrome (SIRS) and it is known that vitamin D may modulate the cytokine ... The hemodynamic shock re- sponse to induction of sepsis may therefore be reduced by administration of 1,25-vit D . In addition to these effects, Vitamin D may also inhibit the...

Ngày tải lên: 26/10/2012, 09:57