Báo cáo y học: "Isolation and characterization of microparticles in sputum from cystic fibrosis patients" pot

... detectable in sputa obtained from CF patients and are predominantly of granulocyte origin. This novel isolation method for MPs from sputum opens a new opportunity for the study of lung pathology in ... efficacy of novel therapies [31]. Sputum is a rich, non-invasive source of biomarkers of inflammation and infection, and has been used extensively to assess inflam...

Ngày tải lên: 12/08/2014, 11:22

... FEBS 2002 mass ranging from 95 to over 100 kDa in polyacrylamide gels. Antibody staining of sections from bovine prelactating and lactating mammary gland using monoclonal and polyclonal antibodies ... 2-propanol were collected and freeze- dried. Standard procedures were employed analysing pro- tein samples by SDS/PAGE using 18% polyacrylamide gels, and for the staining of pr...

Ngày tải lên: 24/03/2014, 04:21

... proteins [8,23] and to identify in vitro the most reactive internal cysteine (Cys207) of Sorghum NADP-MDH [12]. The cytosolic Trx h of Chlamydomonas has only two cysteines in its primary sequence, ... 2Cys-Prx subgroup, and 1Cys-Prx subgroup contain two and one conserved cysteines, respectively. 2Cys-Prx proteins are reduced by the AhpF protein in bacteria, and by the thiore...

Ngày tải lên: 08/03/2014, 16:20

... proteins. By in creasing the temperature of the heat treatment to 70°C, band X faded, whereas the intensity of band Y increased. With further increases in temperature, band Y faded, whereas the intensity ... glycine, and 25 mM Tris. N-terminal amino acid sequence analysis and amino acid sequence homology search After SDS-PAGE, proteins in the polyacrylamide gels were electro...

Ngày tải lên: 12/08/2014, 01:21

... University of Minnesota, Minneapolis, Minnesota, USA and 2 Dept. of Genetics, Cell Biology and Development and the Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, ... efficiency was determined at the hypoxan- thine guanine phosphoribosyl transferase (HPRT) locus and at the adenine phosphoribosyltransferase locus by plating in medium containing...

Ngày tải lên: 13/08/2014, 05:22

... penicillin and 100 μg/ml streptomycin and were used throughout this study. DEV CHv strain was a high-virulence field strain isolated from china, obtained from Key Laboratory of Animal Disease and ... specific band of approximately 35 kDa in infected cell lines (lane 3). However, no signal was present in uninfected cell lines (lane 4). Nucleotide sequence analysis of coding...

Ngày tải lên: 12/08/2014, 04:21

... 2 proteins are cytotoxic due to the presence of a B-chain that mediates surface binding and entry of holotoxin into susceptible cells. From studies of the biosynthesis of type 2 RIPs in their ... contain at least seven closely related and highly toxic type 2 ribosome-inactivating pulchellins, each consisting of a toxic A-chain linked to a sugar binding B-chain. In the pres...

Ngày tải lên: 18/02/2014, 16:20

... classes. Role of Tyr183 in catalysis In previous crystallographic studies it was shown that the hydroxyl side chain of Tyr183 is within hydrogen bonding distance of the N-acetyl carbonyl oxygen of the ... protein as judged by SDS/ PAGE. The activity of the pure recombinant protein was 80% of that of t he wild-type protein in both the lysozyme and chitinase assays. Attemp...

Ngày tải lên: 22/02/2014, 04:20

... the phytotoxic peptide malformin, produced by Aspergillus niger, has the essential structure of a cyclic pentapeptide containing d-cysteine: cyclo-d-cysteinyl d-cysteinyl l-amino acid d-amino acid ... proteins are also present in the mitochondria and the cytoplasm [28,29]. From a physiological point of view the regulation of the cysteine pool by cysteine desulfhydrases in all co...

Ngày tải lên: 16/03/2014, 18:20

... eliminate the possibility of disulphide bond formation genetically by replacement of residues Cys22 and Cys82 with alanine and valine, respectively, to give a cysteine minus mutant (12F-11DCys). ... exception of the CDR loop diversity. Specificity and binding activity of recombinant protein 12F-11 The specificity of protein 12F-11 for Tom70 was demon- strated by ELISA (Fig. 5A)....

Ngày tải lên: 17/03/2014, 10:20