Báo cáo y học: "Isolation and characterization of microparticles in sputum from cystic fibrosis patients" pot
Báo cáo y học: "Isolation and characterization of microparticles in sputum from cystic fibrosis patients" pot
... detectable in sputa obtained from CF patients and are predominantly of granulocyte origin. This novel isolation method for MPs from sputum opens a new opportunity for the study of lung pathology in ... efficacy of noveltherapies [31]. Sputum is a rich, non-invasive source of biomarkers of inflammation and infection, and has beenused extensively to assess inflammation in the CF airways(reviewed ... lymphocytes) following chemical (cytokines,thrombin and endotoxin), physical (shear stress and hypoxia) [17] and apoptotic [18] stimuli. One of the firstdescribed roles for MPs was in the initiation...
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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot
... FEBS 2002mass ranging from 95 to over 100 kDa in polyacrylamidegels. Antibody staining of sections from bovine prelactating and lactating mammary gland using monoclonal and polyclonal antibodies ... 2-propanol were collected and freeze-dried. Standard procedures were employed analysing pro-tein samples by SDS/PAGE using 18% polyacrylamidegels, and for the staining of proteins using CoomassieBrilliant ... surface in epithelial cells of the mammarygland. Likewise, the deduced amino-acid sequences of bothbovine and human MUC15 resemble the mucins in havingserine, threonine and proline as the predominant...
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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
... proteins[8,23] and to identify in vitro the most reactive internalcysteine (Cys207) of Sorghum NADP-MDH [12]. Thecytosolic Trx h of Chlamydomonas has only two cysteines in its primary sequence, ... 2Cys-Prx subgroup, and 1Cys-Prx subgroup containtwo and one conserved cysteines, respectively. 2Cys-Prxproteins are reduced by the AhpF protein in bacteria, and by the thioredoxin/thioredoxin ... dif®culty,thioredoxin-de®cient yeast or E. coli mutant strains havebeen used to express a single-cysteine m utant thioredoxinallowing the i solation of thioredoxin targets in y east [8] and Fig....
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Báo cáo y học: " Isolation and characterization of a virus (CvV-BW1) that infects symbiotic algae of Paramecium bursaria in Lake Biwa, Japan" pptx
... proteins. By in creasing the temperature of the heat treatment to 70°C, band X faded, whereasthe intensity of band Y increased. With further increases in temperature, band Y faded, whereas the intensity ... glycine, and 25 mM Tris.N-terminal amino acid sequence analysis and amino acidsequence homology searchAfter SDS-PAGE, proteins in the polyacrylamide gelswere electroblotted onto polyvinylidene ... than those infected by CvV-BW3)using the streptavidin-biotin system, indicating the pro-duction of hyaluronan by CvV-BW1 (Fig. 9).DNA polymerase gene phylogeny of CvV-BW1The DNA polymerase genes,...
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Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf
... University of Minnesota, Minneapolis, Minnesota, USA and 2Dept. of Genetics, Cell Biology and Development and the Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, ... efficiency was determined at the hypoxan-thine guanine phosphoribosyl transferase (HPRT) locus and at the adenine phosphoribosyltransferase locus byplating in medium containing 6-thioguanine (6-TG) ... 42-7cell lineD)E)NUCLEUSCYTOPLASM01x1052x1053x1054x105Kinetics of proviral DNA synthesis in wild-type and 30-2 cellsFigure 5Kinetics of proviral DNA synthesis in wild-type and 30-2...
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Báo cáo khoa học: "Expression and characterization of the UL31 protein from duck enteritis virus" pot
... penicillin and 100 μg/ml streptomycin and were used throughoutthis study. DEV CHv strain was a high-virulence fieldstrain isolated from china, obtained from Key Laboratory of Animal Disease and ... specificband of approximately 35 kDa in infected cell lines (lane3). However, no signal was present in uninfected cell lines(lane 4). Nucleotide sequence analysis of codingsequences of UL31 ... Project, Ministry of Education of China (706050), the Cultivation Fund of the Key Scientific and Technical Innovation Project, department of Education of Sichuan Province (07ZZ028), Sichuan Province...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... 2 proteins are cytotoxic due to the presence of a B-chain that mediates surface binding and entry of holotoxin into susceptible cells. From studies of thebiosynthesis of type 2 RIPs in their ... contain at least seven closely related and highly toxictype 2 ribosome-inactivating pulchellins, each consisting of a toxic A-chainlinked to a sugar binding B-chain. In the present study, four ... 1 in P II and P III may have no functional consequence in relationto carbohydrate binding. From the putative C-terminalsugar binding site (identical in P I and P II but differ-ing by a single...
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... classes.Role of Tyr183 in catalysis In previous crystallographic studies it was shown that thehydroxyl side chain of Tyr183 is within hydrogen bondingdistance of the N-acetyl carbonyl oxygen of the ... protein as judged by SDS/PAGE. The activity of the pure recombinant protein was80% of that of t he wild-type protein in both the lysozyme and chitinase assays. Attempts to further purify therecombinant ... rotated away from the C1 atom of t hat residue.The c ombined s tructural and k inetic data sh ow thatAsp125 and Tyr183 contribute to catalysis by positioningthe carbonyl oxygen of the N-acetyl group...
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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx
... the phytotoxic peptide malformin, produced byAspergillus niger, has the essential structure of a cyclicpentapeptide containing d-cysteine: cyclo-d-cysteinyld-cysteinyl l-amino acid d-amino acid ... proteins are also present in the mitochondria and the cytoplasm [28,29]. From aphysiological point of view the regulation of thecysteine pool by cysteine desulfhydrases in all compart-ments of ... d-CDesprotein degraded d-cysteine to pyruvate, H2S, and NH3. Interestingly, the PLP-dependent d-selenocystinea,b-lyase from Clostridium sticklandii decomposes d-se-lenocystine into pyruvate,...
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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx
... eliminate the possibility of disulphide bondformation genetically by replacement of residues Cys22 and Cys82 with alanine and valine, respectively, to give acysteine minus mutant (12F-11DCys). ... exception of the CDR loop diversity.Specificity and binding activity of recombinantprotein 12F-11The specificity of protein 12F-11 for Tom70 was demon-strated by ELISA (Fig. 5A). Recombinant protein ... As an initial step in producing ÔintrabodyÕ variants of 12F-11, the impact of modifying or removing the conserved immunoglobulinintradomain disulphide bond was assessed. High affinitybinding was...
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Từ khóa: Báo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀM