Báo cáo y học" Increased plasma membrane cholesterol in cystic fibrosis cells correlates with CFTR genotype and depends on de novo cholesterol synthesis" pdf

... provided the original work is properly cited. Research Increased plasma membrane cholesterol in cystic fibrosis cells correlates with CFTR genotype and depends on de novo cholesterol synthesis Danjun ... that Cftr- null cells and tissues exhibit alterations in cholesterol processing including perinuclear cholesterol accumulation, increased de...

Ngày tải lên: 12/08/2014, 11:22

... levels in patients with MS and depression. Accordingly, the present study was designed in order to assess Hcy plasma levels in MS patients and investigate whether an association with depression and ... Beck Depression Inventory; EDSS, Extended Disability Status Scale; Hcy, plasma homocysteine; RR-MS, relapsing/remitting MS. Correlation graph between plasma homocysteine...

Ngày tải lên: 08/08/2014, 23:21

... therapy, the plasma concentrations of sMer decreased in parallel to the decrease in SLEDAI score. Conclusions: The plasma concentrations of sMer and sTyro3 were significantly increased in patients ... the cytokine production of these cells [7,29]. Under normal conditions, the two vitamin K-dependent proteins Gas6 and protein S bind to the negatively charged phospholipid phos...

Ngày tải lên: 12/08/2014, 15:23

... 87(3):854-60. 4. Jeon HK, So YK, Yang JH, Jeong HS: Extracorporeal oxygenation support for curative surgery in a patient with papillary thyroid carcinoma invading the trachea. J Laryngol Otol 2009, ... France. Authors’ contributions RS conceived, supervise, wrote the article. JS participated in its design, writing process and bibliography MS, KA, participated in its coordination an...

Ngày tải lên: 10/08/2014, 09:21

... of memory B cells and plasma cells, and a continued presence of memory B cells, as shown in the current smokers in our study. Because this increase in memory B cells is only present in the current ... isolations, the flow cytometry analyses and was involved in the immunocytochemical analyses. WG performed the immunocytochemical stainings and analyses. WT and DS wer...

Ngày tải lên: 12/08/2014, 14:20

... Germany) as previously described [25]. After hypotonic lysis to remove contaminating erythro- cytes, cells were suspende d in phosphate-buffered saline (PBS). Purity and viabili ty were routinely ... cytotoxicity, hepatocyte destruction and mortality in mice through the interaction with hepatocyte Fas [16,17] and might contribute to system ic tissue destruction during inflam - m...

Ngày tải lên: 14/08/2014, 07:21

... surface plasmon resonance assay (Surfactant P20, amine coupling reagents, N¢-ethyl-N¢-(dimethylaminopro- pyl)carbodiimide, N-hydroxysuccinimide, ethanolamine hydrochloride) were obtained from Pharmacia ... the dissociation constant. Gel mobility shift assay A possibility of eEF1AÆGDP in forming the complex with deacylated tRNA was studied by nondenaturing PAGE. The samples containing 10 l...

Ngày tải lên: 21/02/2014, 15:20

... H 2 O 2 and is clearly demonstrated by the nonlinear dependence of the observed rate constant on peroxide concentration. A comparison of rate constants for rAPX and variant proteins is only possible ... hydrogen- bonding interactions involving Arg38 in rAPX have been simultaneously disrupted as a secondary consequence of the His42 mutations and, together with His42, may be in uen-...

Ngày tải lên: 18/03/2014, 01:20

... Sections were incubated with mouse anti-osteopontin monoclonal antibody (clone 190312. MAB1433) (R&D Systems, Inc. MN, USA) for 2 hrs, followed by incub ation with a sec- ondary antibody (peroxidase ... Represen- tative pictures were recorded prior to destaining the slides of hematoxylin with 1% HCl in 70% ethanol. The hematoxylin destained samples were assessed in a blinded fas...

Ngày tải lên: 08/08/2014, 21:20

... Using dimethylmethylene blue assay and sulfate incorporation, Arner found that both single and multiple injections of IL-1 led to an initial depression in GAG synthesis rate and a slight drop in ... recovery of explants following trypsin-induced GAG depletion using dGEMRIC, with the increases in [GAG] occurring most rapidly during the first week and slowing considerably after...

Ngày tải lên: 09/08/2014, 01:21