Báo cáo y học: "Cigarette smoke promotes dendritic cell accumulation in COPD; a Lung Tissue Research Consortium study" pdf
Báo cáo y học: "Cigarette smoke promotes dendritic cell accumulation in COPD; a Lung Tissue Research Consortium study" pdf
... this article as: Vassallo et al., Cigarette smoke promotes dendritic cell
accumulation in COPD; a Lung Tissue Research Consortium study Respiratory
Research 2010, 11:45
Vassallo et al. Respiratory ... in
COPD.
Virtually all lung compartments (conducting airways,
interstitial and alveolar spaces, vascular structures, and
pleura) contain dendritic cells [4]. Wher...
Báo cáo y học: "Cigarette smoke regulates the expression of TLR4 and IL-8 production by human macrophages" pdf
... IRAK-1 and degradates IκB-α [8]. In this study
by using NAC, we have demonstrated that ROS play an
important role in CSM-induced TLR4 associated intracel-
lular signaling. Interestingly, we have ... efficiency correction using a PCR data anal-
ysis program, qBase to obtain relative quantification val-
ues.
Protein Assay
The protein content of the lyzate was determined using
the bicincho...
Báo cáo y học: "Cigarette smoke exposure facilitates allergic sensitization in mice" pps
... responses against
harmless antigens. Our data suggest that cigarette smoke
can be depicted not only as an aggravating factor for exist-
ing airway inflammation [5], but also as a facilitating and
Goblet ... Yoneyama H, Nakayama T, Fujisawa R, Izu-
mizaki M, Imai T, Yoshie O, Homma I, Yamamoto K, Matsushima K:
Intervention of thymus and activation-regulated chemokine
attenuates the develop...
Báo cáo y học: " Airway inflammation contributes to health status in COPD: a cross-sectional study" pps
... may be dynamic hyperinflation, i.e.
increased residual volume and total lung capacity [7], pos-
sibly as a consequence of chronic inflammation and
restructuring of the airways and/or parenchyma ... the
underlying local airway inflammation in COPD can drive
impairment of health status as well [12].
We hypothesized that health status in COPD is affected by
the severity of airway inflamm...
Báo cáo y học: "Cigarette Smoke Exposure Alters mSin3a and Mi-2α/β Expression; implications in the control of pro-inflammatory gene transcription and glucocorticoid function" ppt
... HDAC-2 antibody (Santa Cruz Biotechnology, CA,
USA); mSin 3a antibody (Abcam, Cambridge, UK); Mi2α/
β antibody (Austral Biotechnology, San Ramon, CA,
USA); Lamin A/ C antibody (Santa Cruz); GAPDH
(Abcam).
Statistical ... GAPDH
(Abcam).
Statistical Analysis
Data was analysed by 1 way ANOVA to determine statis-
tical significant variance between the groups for each
endpoint assessed. Statisti...
Báo cáo y học: "Cigarette Smoke Exposure Alters mSin3a and Mi-2α/β Expression; implications in the control of pro-inflammatory gene transcription and glucocorticoid function" pdf
... HDAC-2 antibody (Santa Cruz Biotechnology, CA,
USA); mSin 3a antibody (Abcam, Cambridge, UK); Mi2α/
β antibody (Austral Biotechnology, San Ramon, CA,
USA); Lamin A/ C antibody (Santa Cruz); GAPDH
(Abcam).
Statistical ... GAPDH
(Abcam).
Statistical Analysis
Data was analysed by 1 way ANOVA to determine statis-
tical significant variance between the groups for each
endpoint assessed. Statisti...
Báo cáo y học: "Cigarette smoke regulates VEGFR2-mediated survival signaling in rat lungs" pot
... signaling in rat lungs
John A Marwick
1,2
, Indika Edirisinghe
4
, Gnanapragasam Arunachalam
4
, Christopher S Stevenson
1,2
,
William MacNee
3
, Paul A Kirkham
1,2*
, Irfan Rahman
4*
Abstract
Background: ... activation of
caspase 3 or lung cell death
Increased endothelial cell death was observed in emphy-
sematous lungs of smokers indicate that apoptotic cell
death may play...
Báo cáo y học: " Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells" pps
... Sense hairpin siRNA (ACA
CTA CAC AAA TGT TCC ACT GGG CTG AGA ACC
GGT GTT TCG TCC TTT CCA CAA G) and anti-
sense hairpin siRNA (CGG CGA AGC TTT TTC CAA
AAA ATT CTC AGC CCA GTG GAA CAC TAC ACA
AAT ... inflammation and tissue remodeling in mouse
allergic asthma.
Figure 8 A schematic diagram of signaling pathway in mast
cells activated by antigen/antibody reaction and smoke
exposure in...
Báo cáo y học: " The mode of lymphoblastoid cell death in response to gas phase cigarette smoke is dose-dependent" potx
... confocal
microscopy images. GB was involved in the flow cytome-
try analysis. DGH realized the gas chromatography analy-
ses for the obtained GPS samples. All authors read and
approved the final manuscript.
Acknowledgements
We ... analysis
All data presentations (graphs etc.) and corresponding
statistical analysis was performed using SigmaPlot and
SigmaStat software packages (SPSS Inc.). Al...
Báo cáo y học: " Epithelial expression of TLR4 is modulated in COPD and by steroids, salmeterol and cigarette smoke" pot
... densitome-
try and compared in a semi quantitative manner relative
to GAPDH using ImageMaster
®
TotalLab Software (Amer-
sham Pharmacia, Amersham, UK).
Real Time PCR
TLR4 mRNA was quantified using commercially ... extracts (10 μg) from
A5 49 cells probed with an anti-TLR4 or anti-Actin antibody.
Equal protein loading and transfer efficiency was confirmed
by Ponceau S staining. Data ar...