... homology 2 domains (a) the growth factor receptor binding protein activates the ras/ mitogen-activated protein kinase pathway and (b) phosphatidylinositol 3 -kinase activates the protein kin- ase B/glycogen ... The protein kinase C pseudosubstrate peptide (PKC19-36) inhibits insulin-stimulated protein kinase activity and insulin-mediated translocation of the glucose...
Ngày tải lên: 20/02/2014, 02:21
... targeting det erminants for chloroplasts and mito- chondria are usually located at the N-terminus of the protein; therefore, a fusion protein with GFP fused to the C-terminus of TSPO was made. Using ... increased in gun2 (allele of hy1 - required for phytochromobilin synthesis from heme) [35], gun4 (mutant in the Proto- porphyrin I X- and Mg-Protoporphyrin IX-binding pro- tein)...
Ngày tải lên: 11/08/2014, 11:21
báo cáo khoa học: " Conifer R2R3-MYB transcription factors: sequence analyses and gene expression in wood-forming tissues of white spruce (Picea glauca)" pot
... [18,30,31], including the bHLH-interaction and calmodulin-binding motifs in the DNA-binding domain [28,29], and the repression domain pdLNLD/ELxiG/S in the C-terminus [7]. In our study, the spruce ... required for MYB proteins to transactivate some of the phenylpropanoid and anthocyanin genes through protein- protein interactions [32]. The G motif in the C- terminus h...
Ngày tải lên: 12/08/2014, 05:20
Báo cáo khoa học: The SWI⁄SNF protein BAF60b is ubiquitinated through a signalling process involving Rac GTPase and the RING finger protein Unkempt doc
... IgG. The expression of transfected proteins was monitored on total protein extracts by immunoblotting using the indicated antibodies. Where indicated, cells were treated with the proteasome inhibitor ... ligase (E3), acting sequentially to form an isopeptide bond between the ubiquitin C-terminus and the e-amino group of lysines of the protein substrate; ubiquitin contains se...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: The nucleosome-binding protein HMGN2 modulates global genome repair potx
... left corner of the plot, and the G 2 –M cells in the right side of the plot. The cells in S-phase are between these two groups, in the arch of the ‘horseshoe’, which is high in BrdU. The results ... leads to an increase in both the UV-induced rate of apoptosis and in the level of checkpoint arrest. In GGR, HMGN2 and HMGN1a proteins were, for the most part,...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: The optimization of protein secondary structure determination with infrared and circular dichroism spectra docx
... for just over half of the proteins in the RaSP50 set. If we consider the remaining proteins, overall the larger determined FC H was more accurate for 74% of the proteins in this second RaSP50 ... of the protein listed in the SWISS-PROT database [38,39]. The side chain spectrum was then subtracted from the protein spectrum u sing a scaling factor determined by Fouri...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: The transthyretin-related protein family doc
... transthyretin and retinol-binding protein. Science 268, 1039–1041. 8. Naylor, H.M. & Newcomer, M.E. (1999) The structure of human retinol-binding protein (RBP) with its carrier protein transthyretin reveals ... expressed specifically in the intestine [39], suggesting that R09H10.3 might be expressed in this tissue. RNA interference in C. elegans In C. elegans injection of...
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: The multi-replication protein A (RPA) system – a new perspective ppt
... recombinases and the DNA helicases, Bloom’s and Werner’s proteins. RPA is also a checkpoint protein that has been iden- tified by the generation of a mutant in the large sub- unit in yeast [92]. In ... [124]. Indeed, RPA70kDa is the major subunit of the complex having four ssDNA- binding domains in the middle of the subunit. By contrast, RPA32kDa and RPA14kDa each posse...
Ngày tải lên: 23/03/2014, 06:20
Báo cáo khoa học: Differential involvement of protein kinase C alpha and epsilon in the regulated secretion of soluble amyloid precursor protein docx
... data in the literature that indicate PKCe as the only protein kinase isoform involved in the s ignalling pathway downstream of muscarinic m3 receptors in SK-N-BE(C) neuroblasto ma cells [15]. The Ó ... overexpres- sion of the PKCe V1 region, which binds specifically to the receptor for activated C -kinase (RACK), blocking the activation of the kinase specifically [1...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: The ubiquitin-like protein monoclonal nonspecific suppressor factor b conjugates to endophilin II and regulates phagocytosis potx
... [2,8]. The endophilins are a family of proteins identified in a search for SH3 domain-containing proteins. The most extensively studied isoform of the mammalian endophilins, brain-specific endophilin ... from ribosomal protein S30 in the cytosol [1]. MNSFb is covalently attached to certain lysines of specific target proteins, including Bcl-G, a proapoptotic protein [2]. MNSFb con...
Ngày tải lên: 29/03/2014, 22:21