báo cáo khoa học: " BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut" ppsx

... Central Page 1 of 8 (page number not for citation purposes) BMC Plant Biology Open Access Research article BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated ... describe the production of BAC libraries for the AA and BB genomes of Arachis. The librar- ies will be a useful resource for the isolation of g...

Ngày tải lên: 12/08/2014, 05:20

... predicted from open reading frames of the cloned skin secretion-derived cDNAs from each species. The calculated molecular masses of the predicted peptides were then used to interrogate MALDI-TOF MS ... one of the peptides from O. livida (nigrocin-2LVa) and of nigro- cin-2HJ from O. hejiangensis were identical to that of the original nigrocin-2N peptide from the...

Ngày tải lên: 15/03/2014, 10:20

... presenting the pulse of Src activity in the plane of the inactive and active fractions, s i and s 1 . If the duration of the stimulus exceeds the critical value, the trajectory in the (s i , ... binds to the linker between the SH2 and kinase domains at the back of the small lobe, preventing the formation of a produc- tive catalytic cleft [29]. Thus, these i...

Ngày tải lên: 18/02/2014, 11:20

... prevent degradation. A common drawback of such approaches is the cost of the affinity resins used to isolate the fusion protein and the proteases required to liberate the protein of interest from the fusion protein. ... [41]. The use of thrombin significantly increases the cost of the purification, and the require- ment for HPLC increases the length and complexi...

Ngày tải lên: 18/02/2014, 13:20

... pro- teins, but we expect that the relative expression level of the tagged proteins is indicative of that of the equival- ent untagged proteins. To assess the function of these proteins in endo- cytosis ... direct effect of the loss of residues 465–492 rather than an indirect consequence of reduced C-Vrp1p 493)817 expression levels. We were not able to assay the expr...

Ngày tải lên: 18/02/2014, 16:20

... encoded another type of KLK11, isoform 2, despite the presence of exon 1c. The secretory path- ways of KLK11 isoforms appeared to differ, although both isoforms were secreted from cells. Hence, the ... part of isoform 2 mRNA. The third exon, exon 1c, extended from +1379 to +1454. The noncoding exons of exons 1a and 1c contributed to the 5¢ nontranslated region of i...

Ngày tải lên: 19/02/2014, 06:20

... synthesized in five steps from D -pantothenate. The Dfp flavoprotein catalyzes the synthe- sis of the coenzyme A precursor 4¢-phosphopantetheine from 4¢-phosphopantothenate and cysteine using the ... has Fig. 5. Analysis of the enzymatic activity of His-CoaB R206Q and A276V. The synthesis of 4¢-phosphopantothenoyl-CMP (–cysteine, upper part of the figure) and of 4¢-...

Ngày tải lên: 19/02/2014, 12:20

... an assay of Ras-dependent neurite outgrowth from PC12 cells [14], may not be detectable at the level of ERK1 ⁄ 2 phosphorylation owing to amplification of the signal through the kinase cascade. These ... decreases upon the binding of Ca 2+ . The change in the ratio of F340 ⁄ F380 determined the change in Ca 2+ concentration inside the cell. Acknowledgements This wor...

Ngày tải lên: 19/02/2014, 18:20

... the scale of the Bard’s genera- tivity, and the quality of its insights. 5 Empirical Evaluation The vastness of the web, captured in the large- scale sample that is the Google ngrams, means the Jigsaw ... the first case, predictions of the pleasantness of stereotype nouns based on the pleasantness of their salient properties (i.e., predicting the pleasantne...

Ngày tải lên: 20/02/2014, 05:20

... are m ainly regulated at the transcriptional level. This report s hows that the b FGF r egulation of their expression speci®cally depends on the nature of HSPG and of the Sertoli cell developmental stage. In ... (HSPG) on the cell surface [11]. Oligosaccharidic sequences of HS chains are de®ned for the bFGF binding and for the recognition of the speci®c bFGF receptor...

Ngày tải lên: 21/02/2014, 03:20