báo cáo khoa học: " BAC libraries construction from the ance

báo cáo khoa học: " BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut" ppsx

... Central Page 1 of 8 (page number not for citation purposes) BMC Plant Biology Open Access Research article BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated ... describe the production of BAC libraries for the AA and BB genomes of Arachis. The libraries will be a useful resource for the isolation of g...

Ngày tải lên : 12/08/2014, 05:20

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Báo cáo khoa học: Nigrocin-2 peptides from Chinese Odorrana frogs – integration of UPLC/MS/MS with molecular cloning in amphibian skin peptidome analysis pot

... predicted from open reading frames of the cloned skin secretion-derived cDNAs from each species. The calculated molecular masses of the predicted peptides were then used to interrogate MALDI-TOF MS ... one of the peptides from O. livida (nigrocin-2LVa) and of nigrocin-2HJ from O. hejiangensis were identical to that of the original nigrocin-2N peptide from the...

Ngày tải lên : 15/03/2014, 10:20

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Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc

... presenting the pulse of Src activity in the plane of the inactive and active fractions, s i and s 1 . If the duration of the stimulus exceeds the critical value, the trajectory in the (s i , ... binds to the linker between the SH2 and kinase domains at the back of the small lobe, preventing the formation of a produc- tive catalytic cleft [29]. Thus, these i...

Ngày tải lên : 18/02/2014, 11:20

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Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... prevent degradation. A common drawback of such approaches is the cost of the affinity resins used to isolate the fusion protein and the proteases required to liberate the protein of interest from the fusion protein. ... [41]. The use of thrombin significantly increases the cost of the purification, and the require- ment for HPLC increases the length and complexi...

Ngày tải lên : 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc

... proteins, but we expect that the relative expression level of the tagged proteins is indicative of that of the equival- ent untagged proteins. To assess the function of these proteins in endocytosis ... direct effect of the loss of residues 465–492 rather than an indirect consequence of reduced C-Vrp1p 493)817 expression levels. We were not able to assay the expr...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-speciﬁc alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt

... encoded another type of KLK11, isoform 2, despite the presence of exon 1c. The secretory path- ways of KLK11 isoforms appeared to differ, although both isoforms were secreted from cells. Hence, the ... part of isoform 2 mRNA. The third exon, exon 1c, extended from +1379 to +1454. The noncoding exons of exons 1a and 1c contributed to the 5¢ nontranslated region of i...

Ngày tải lên : 19/02/2014, 06:20

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Tài liệu Báo cáo khóa học: Active-site residues and amino acid speciﬁcity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

... synthesized in five steps from D -pantothenate. The Dfp flavoprotein catalyzes the synthesis of the coenzyme A precursor 4¢-phosphopantetheine from 4¢-phosphopantothenate and cysteine using the ... has Fig. 5. Analysis of the enzymatic activity of His-CoaB R206Q and A276V. The synthesis of 4¢-phosphopantothenoyl-CMP (–cysteine, upper part of the figure) and of 4¢-...

Ngày tải lên : 19/02/2014, 12:20

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Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... an assay of Ras-dependent neurite outgrowth from PC12 cells [14], may not be detectable at the level of ERK1 ⁄ 2 phosphorylation owing to ampliﬁcation of the signal through the kinase cascade. These ... decreases upon the binding of Ca 2+ . The change in the ratio of F340 ⁄ F380 determined the change in Ca 2+ concentration inside the cell. Acknowledgements This wor...

Ngày tải lên : 19/02/2014, 18:20

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Tài liệu Báo cáo khoa học: "Exploiting Readymades in Linguistic Creativity: A System Demonstration of the Jigsaw Bard" docx

... the scale of the Bard’s genera- tivity, and the quality of its insights. 5 Empirical Evaluation The vastness of the web, captured in the large- scale sample that is the Google ngrams, means the Jigsaw ... the first case, predictions of the pleasantness of stereotype nouns based on the pleasantness of their salient properties (i.e., predicting the pleasantne...

Ngày tải lên : 20/02/2014, 05:20

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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx

... are m ainly regulated at the transcriptional level. This report s hows that the b FGF r egulation of their expression speci®cally depends on the nature of HSPG and of the Sertoli cell developmental stage. In ... (HSPG) on the cell surface [11]. Oligosaccharidic sequences of HS chains are de®ned for the bFGF binding and for the recognition of the speci®c bFGF receptor...

Ngày tải lên : 21/02/2014, 03:20

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