báo cáo khoa học: " The red fluorescent protein eqFP611: application in subcellular localization studies in higher plants" doc

báo cáo khoa học: " The red fluorescent protein eqFP611: application in subcellular localization studies in higher plants" doc

báo cáo khoa học: " The red fluorescent protein eqFP611: application in subcellular localization studies in higher plants" doc

... Biology Open Access Research article The red fluorescent protein eqFP611: application in subcellular localization studies in higher plants Joachim Forner and Stefan Binder* Address: Molekulare Botanik, ... fluorescent proteins have revolutionized studies in molecular cell biology. The parallel application of these proteins in dual- or multilabeling experime...
Ngày tải lên : 12/08/2014, 05:20
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Tài liệu Báo cáo khoa học: The diacylglycerol and protein kinase C pathways are not involved in insulin signalling in primary rat hepatocytes doc

Tài liệu Báo cáo khoa học: The diacylglycerol and protein kinase C pathways are not involved in insulin signalling in primary rat hepatocytes doc

... homology 2 domains (a) the growth factor receptor binding protein activates the ras/ mitogen-activated protein kinase pathway and (b) phosphatidylinositol 3-kinase activates the protein kin- ase B/glycogen ... The protein kinase C pseudosubstrate peptide (PKC19-36) inhibits insulin-stimulated protein kinase activity and insulin-mediated translocation of the glucose transpo...
Ngày tải lên : 20/02/2014, 02:21
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Báo cáo khoa học: The optimization of protein secondary structure determination with infrared and circular dichroism spectra docx

Báo cáo khoa học: The optimization of protein secondary structure determination with infrared and circular dichroism spectra docx

... for just over half of the proteins in the RaSP50 set. If we consider the remaining proteins, overall the larger determined FC H was more accurate for 74% of the proteins in this second RaSP50 ... of the protein listed in the SWISS-PROT database [38,39]. The side chain spectrum was then subtracted from the protein spectrum u sing a scaling factor determined by Fouri...
Ngày tải lên : 16/03/2014, 18:20
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báo cáo khoa học: " The Arabidopsis translocator protein (AtTSPO) is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism" potx

báo cáo khoa học: " The Arabidopsis translocator protein (AtTSPO) is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism" potx

... saline). The reaction was further conducted at 37°C in the dark for a maximum of 16 hours. Subcellular localization of AtTSPO fusion proteins For the GFP fusion constructs, clones containing the coding ... increased in gun2 (allele of hy1 - required for phytochromobilin synthesis from heme) [35], gun4 (mutant in the Proto- porphyrin I X- and Mg-Protoporphyrin IX-binding pr...
Ngày tải lên : 11/08/2014, 11:21
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Báo cáo khoa học: The SWI⁄SNF protein BAF60b is ubiquitinated through a signalling process involving Rac GTPase and the RING finger protein Unkempt doc

Báo cáo khoa học: The SWI⁄SNF protein BAF60b is ubiquitinated through a signalling process involving Rac GTPase and the RING finger protein Unkempt doc

... IgG. The expression of transfected proteins was monitored on total protein extracts by immunoblotting using the indicated antibodies. Where indicated, cells were treated with the proteasome inhibitor ... was resuspended in Laemmli sample buffer 2X to evaluate the total quantity of proteins. 6His-myc-ubiquiti- nated proteins were recovered by incubating the remaining lysate for 9...
Ngày tải lên : 06/03/2014, 09:22
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Báo cáo khoa học: The nucleosome-binding protein HMGN2 modulates global genome repair potx

Báo cáo khoa học: The nucleosome-binding protein HMGN2 modulates global genome repair potx

... left corner of the plot, and the G 2 –M cells in the right side of the plot. The cells in S-phase are between these two groups, in the arch of the ‘horseshoe’, which is high in BrdU. The results ... impairs the rate of UV- induced GGR. Loss of HMGNs leads to an increase in both the UV-induced rate of apoptosis and in the level of checkpoint arrest. In GGR,...
Ngày tải lên : 07/03/2014, 00:20
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Báo cáo khoa học: The transthyretin-related protein family doc

Báo cáo khoa học: The transthyretin-related protein family doc

... transthyretin and retinol-binding protein. Science 268, 1039–1041. 8. Naylor, H.M. & Newcomer, M.E. (1999) The structure of human retinol-binding protein (RBP) with its carrier protein transthyretin reveals ... a-helix [4,5]. The dimer– dimer association creates a central hydrophobic channel where the two hormone-binding sites are situated [6], while the two retinol-binding...
Ngày tải lên : 17/03/2014, 09:20
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Báo cáo khoa học: The multi-replication protein A (RPA) system – a new perspective ppt

Báo cáo khoa học: The multi-replication protein A (RPA) system – a new perspective ppt

... [124]. Indeed, RPA70kDa is the major subunit of the complex having four ssDNA- binding domains in the middle of the subunit. By contrast, RPA32kDa and RPA14kDa each possess a single DNA-binding ... domain, displaying only weak binding affinity [2,125]. The contact surfaces in RPA have been elucidated for several of its binding part- ners. The results of these studies suggest...
Ngày tải lên : 23/03/2014, 06:20
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Báo cáo khoa học: The ubiquitin-like protein monoclonal nonspecific suppressor factor b conjugates to endophilin II and regulates phagocytosis potx

Báo cáo khoa học: The ubiquitin-like protein monoclonal nonspecific suppressor factor b conjugates to endophilin II and regulates phagocytosis potx

... [2,8]. The endophilins are a family of proteins identified in a search for SH3 domain-containing proteins. The most extensively studied isoform of the mammalian endophilins, brain-specific endophilin ... modification is involved in the phagocytosis of zymosan in Raw264.7 cells. Thus, as in the case of endophilin III, the variable region of endophilin II may be important in...
Ngày tải lên : 29/03/2014, 22:21
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Báo cáo khoa học: The heat shock protein 70 molecular chaperone network in the pancreatic endoplasmic reticulum ) a quantitative approach potx

Báo cáo khoa học: The heat shock protein 70 molecular chaperone network in the pancreatic endoplasmic reticulum ) a quantitative approach potx

... pep- tides at the N-terminus of the precursor proteins and the transport machinery. Post-translational protein transport into the yeast ER involves the Sec complex in the membrane, comprising the Sec61p ... luminal protein that is involved in the completion of protein translocation [14,15]. Furthermore, BiP was shown to seal the lumi- nal end of the mammalian Sec...
Ngày tải lên : 30/03/2014, 03:20
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