báo cáo khoa học: " The PTI1-like kinase ZmPti1a from maize (Zea mays L.) co-localizes with callose at the plasma membrane of pollen and facilitates a competitive advantage to the male gametophyte" doc

... proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species Takla G. El-Khoury, Georges M. Bahr and Karim ... correlating the uncoupling effectinduced by these molecules with the level and function of UCP2 and free radical production in macrophages. We find that MDP induces reactive oxygen and nitro-gen species ... Stimulation of cells with MDP, but not with murabutide, rapidly upregulates the expres-sion of the mitochondrial protein uncoupling protein 2 (UCP2), and pre-treatment with vitamin E attenuates upregulation...

...

... 5Â-CACTTGAAGCTTTAAGGAGGAAtagACCATGCGTATCCGTGAGCTTGGCATCACC-3Â;antisen se primer, 5Â-ACGCAATCTAGAGTCAGCCCTCAGGGGGCTTTCG-3Â. The amplied PCR product wasdigested with HindIII and XbaI (Takara ... L-Ala-(Gly)2(L-Ala)2, L-Ala-D-Ala, L-Ala-D-Ala-L-Ala,DL-Ala-DL-Asn, DL-Ala-DL-Ile, DL-Ala-DL-Leu, DL-Ala-DL-Met, DL-Ala-DL-Phe, DL-Ala-DL-Ser, DL-Ala-DL-Val, L-Asp-D-Ala, L-Pro-Gly, L-Pro-L-Phe, c-Aminobutyryl-L-His ... of BapA and DmpA. The following compounds were not substrates for BapA:(Gly)2(Gly)3, D-Ala-Gly, D-Ala-(Gly)2(D-Ala)2, D-Ala-L-Ala (D-Ala)3(D-Ala)4, L-Ala-Gly, L-Ala-(Gly)2(L-Ala)2,...

... that the catalytic sites of the enzyme are functional and are distinguishable on the basis of bind-ing with the inhibitor. Careful analysis of Fig. 2 showsthat inactivation of E3by trypsin and ... Council of Scientic and Industrial Research, New Delhi. Journal compilation ê 2009 FEBS 6727 UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional ... inactivation by 50% in thesereactions indicated that the catalytic sites of epimerase are nonidentical.Equation (2) was used further to assess the perfor-mance of the catalytic sites of the inhibitor-free...

... et al.(Eur. J. Biochem. 271) Ó FEBS 2004 Chloroplast phosphoglycerate kinase from Euglena gracilis Endosymbiotic gene replacement going against the tide Ulrich Nowitzki1, Gabriel Gelius-Dietrich1, ... homologues from animalsand f ungi.Keywords: endosymbiotic gene replacement; Euglena graci-lis; phosphoglycerate kinase; p olyproteins. The complex chloroplasts of the photosynthetic flagellate Euglena ... Enolase from Trypanosoma brucei,fromtheamitochondriate protist Mastigamoeba balamuthi, and from the chloroplast and the cytosol of Euglena gracilis: pieces in the evo-lutionary p uzzle o f the...

... AOP-1 (Eur. J. Biochem. 270)79 Mixed lineage kinase LZK and antioxidant protein-1 activate NF-jB synergistically Megumi Masaki, Atsushi Ikeda, Eriko Shiraki, Shogo Oka and Toshisuke KawasakiDepartment ... kawasaki@pharm.kyoto-u.ac.jpAbbreviations: LZK, leucine zipper-bearing kinase; AOP-1, antioxidant protein-1; MLK, mixed lineage kinase; JNK, c-Jun NH2terminal kinase; MAPK, mitogen-activated protein kinase; MAPKK, MAPK kinase; ... Rac1 and Cdc42 to the c-Jun N-terminal kinase/ stress-acti-vated protein kinase pathway. A role for mixed lineage kinase 3/protein-tyrosine kinase 1, a novel member of the mixed lineage kinase...

... consists of picking the most likely translation from the translation candidates we have generated. To discern the likely from the unlikely, we use the empirical evidence provided by the set of Japanese ... around the seed. 2.2 Automatic Term Recognition The next step is to extract candidate related terms from the corpus. Because the sentences compos-ing the corpus are related to the seed, the ... translation. They use a compositional method to generate a set of translation candidates from which they select the most likely translation by using empirical evidence from the web. The method...

... proteins involved in Nox1 gene inducement in macrophages is not clear. There have been somereports of the Nox1 expression mechanism in macro-phages. Reports show that IRAK -1 increases Nox1 expression ... support issues arisingfrom supporting information (other than missing files)should be addressed to the authors.J S. Kim et al. GSK3b -b-catenin is involved in Nox1 expression FEBS Journal 277 (2 010 ) ... Therefore, we tested changes in GSK3b phosphorylation, using LPS stimulation, in J S. Kim et al. GSK3b -b-catenin is involved in Nox1 expression FEBS Journal 277 (2 010 ) 28302837 ê 2 010 The Authors Journal...

... MINIREVIEW Examining multiprotein signaling complexes from all angles The use of complementary techniques to characterize complex formation at the adapter protein, linker for activation of T cells Jon ... example is the characterization of the hematopoi-etic-specific adapter protein, linker for activation of T cells (LAT). The composition, formation and func-tion of signaling complexes at LAT has ... NF -AT and AP-1 [18,22,26]. Together, this indicates that the formation of multiprotein signaling complexes at LATis crucial for linking LAT phosphorylation to the stimulation of intracellular signaling...

... examined asdetailed in the legend of Fig. 1.Ó FEBS 2004 Cytoplasmic SIK1 represses CREB (Eur. J. Biochem. 271) 4313 Salt-inducible kinase-1 represses cAMP response element-binding protein activity ... C.T. (2001) Calmodulin kinase IIattenuation of gene transcription by preventing cAMP response element-binding protein (CREB) dimerization and binding of the CREB-bind ing protein . J. Biol. Chem. ... another shuttling kinase, is involved in the regulationof translation initiation by activating p70 S6 kinase and phosphorylating eIF-4E-binding protein [52]. When FRAPwas restricted to the nucleus...

... REVIEW ARTICLE Serpins 2005 – fun between the b-sheets Meeting report based upon presentations made at the 4th Interna-tional Symposium on Serpin Structure, Function and Biology (Cairns, Australia) James ... polymerization and con-formational diseases such as the human serpinopathies. This review reports on recent major discoveries in the serpin field, based upon presentations made at the 4th International ... antichymotrypsin, these data sug-gesting that the antichymotrypsin-like serpins areunder conformational control.Another theme of the meeting was the use of modelorganisms to understand serpin function. ...

... Open Access STIL, a peculiar molecule from styles, specifically dephosphorylates the pollen receptor kinase LePRK2 and stimulates pollen tube growth in vitroDiego L Wengier1, Mar a A Mazzella1, ... chemocyanin orits Arabidopsis homolog, plantacyanin [61,62]. Arabino-galactan proteins (AGPs) have also been involved in modulating pollen tube growth in Solanaceous species. A transmitting tissue-specific ... STIL alwayscorrelated with absorbance at 280 nm and amino aciddetermination (Table 3) showed that several amino acidsare present in the STIL molecule. Amino acid determina-tion relies on acid...

... article The PTI1-like kinase ZmPti 1a from maize (Zea mays L.) co-localizes with callose at the plasma membrane of pollen and facilitates a competitive advantage to the male gametophyteMarkus ... (5'-P-gatccatgggatgcttttcatgctgctgtgtggcagat-gacgacaacgttggcaggaggaagaagcat-3') and Myr-B (5'-P-gatcatgcttcttcctcctgccaacgttgtcgtcatctgccacacagcagcat-gaaaagcatcccatg-3') and ligation of the resulting doublestranded DNA into BamHI linearized ... (5'-taataaggtggtcacgaccgctg-3') for ZmPti1c; SP12(5'-ctgcaccaaccaccgaagagccagctcca-3') and SP13 (5'-aacttc-gaaccaacttcatataccatt-3') for ZmPti1d; and -ZAPđ IIcDNA-libraries...