Báo cáo khoa học: "Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA" doc

... preparation The JEV isolate KV1899, Anyang 300 (attenuated vaccine strain) and Nakayama strain were used as standard virus for detection of JEV by real-time RT-PCR. The KV1899 and Anyang 300 strains ... by utilizing the 5-3 exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci USA 1991, 88 , 7276- 7280. 10. Igarashi A, Tanaka M, Morita K, Takasu T, A...

Ngày tải lên: 07/08/2014, 18:20

... Alberta, Canada 2 Department of Clinical Neuroscience, University of Calgary, Calgary, Alberta, Canada 3 Department of Medicine, University of Calgary, Calgary, Alberta, Canada 4 Department of Community ... Health Sciences, University of Calgary, Calgary, Alberta, Canada 5 Department of Surgery, University of Calgary, Calgary, Alberta, Canada Corresponding author: David Zygun,...

Ngày tải lên: 13/08/2014, 02:24

... size of cavities enables a high number of cav- ities on each chip and facilitates the performance of many independent assays on one plate. The funnel- shaped cavities avoid the appearance of meniscuses, ... in each cultivation segment guarantee a statistical analysis of the generated data. The MCC represents an array of miniaturized cell culture chambers for perman...

Ngày tải lên: 21/06/2014, 00:20

... apical staining of this marker was apparent in distinct areas of 3-D Huh7 aggregates (Fig. 3). Taken together, these data demonstrate that the expres- sion and distribution of cell adhesion and TJ ... study and participated in drafting the manu- script. All authors read and approved the final manu- script. Acknowledgements We thank Drs. Heather L. LaMarca and Kerstin Hönzer zu Ben...

Ngày tải lên: 12/08/2014, 04:21

... cell surface ectopeptidase that localizes to the apical plane of polarized cells [45], was non-detectable in 2-D Huh7 monolayers, while, apical staining of this marker was apparent in distinct areas of ... transport in choroid plexus. J Biol Chem 1987, 262:13907-13915. 37. Nakata K, Tanaka Y, Nakano T, Adachi T, Tanaka H, Kaminuma T, Ishikawa T: Nuclear receptor-mediated transcription...

Ngày tải lên: 12/08/2014, 04:22

... and the carboxyl groups available on the surface of the support. Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA) unambiguously proved the existence of covalent bonds ... was just based on organic molecules as fluorescent chemosensors. For a few practical applications the attachment of the fluorescent units to a solid support has advant...

Ngày tải lên: 22/06/2014, 01:20

... Mathematical transformations of ECG data The 3DMP ECG analysis employs six mathematical transformations. All these transformations are based on the power spectrum of the recorded ECG leads ... Asia, and North America on individuals of varying ages and degrees of disease state including normal populations [17,18]. All ECG ana lyses in this database have been validated again...

Ngày tải lên: 08/08/2014, 16:23

... RJ, Abrams J, Chatterjee K, et al. ACC/AHA 2002 guideline update for the management of patients with chronic stable angina summary article: a report of the American College of Cardiology/American ... Beasley JW, et al. ACC/AHA guideline update for the management of patients with unstable angina and non-ST-segment elevation myocardial infarction 2002: summary article:...

Ngày tải lên: 08/08/2014, 17:20

... Hematology, University of Perugia, Perugia, Italy). The forward and backward primers were: 5’-CGGGATCCATCGAAGGTCGTGAAGATTCGAT GGACAT-3’, and 5’-CGCGCGACCGAGCGGAA GCTTCTATTTTCTTAAAGAGAC-3’. Underlined ... clinical diagnosis/prognosis of NPMc+AML, we need to prepare the anti-NPM-mA monoclonal antibodies (mAbs) as the primary antibody in IHC assay. In 1999, Cordell et al prepare...

Ngày tải lên: 08/08/2014, 18:21

... mono- saccharidic composition. The composition of neutral sugars is obtained by HPAE-PAD. Data are represented as ngÆlg )1 of the total matrix and as a percentage of the total identified carbohydrate compounds. ... CaCO 3 precipitation. First, the effect of the nacre matrix started to occur above 1 lg of the ASM and the delay of the reaction was dose- dependent. A...

Ngày tải lên: 22/03/2014, 16:20