Báo cáo khoa học: "Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA" doc

Báo cáo khoa học: "TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus" pot

... preparation The JEV isolate KV1899, Anyang 300 (attenuatedvaccine strain) and Nakayama strain were used as standardvirus for detection of JEV by real-time RT-PCR. The KV1899 and Anyang 300 strains ... byutilizing the 5-3 exonuclease activity of Thermus aquaticusDNA polymerase. Proc Natl Acad Sci USA 1991, 88, 7276-7280.10.Igarashi A, Tanaka M, Morita K, Takasu T, Ahmed A, Ahmed A, Akram DS, Waqar ... 6Fam-TGCCGTGGGCAACGATCCGG-Tamra 645-664JE3F GGTGTA AGGACTAGA GGTTAG AGG 10,726-10,750JE3R ATTCCC AGGTGTCAATATGCTGTT 10,848-10,871 3' NTR 146 bpProbe3 6Fam-CCCGTGGAAACAACATCATGCGGC-Tamra 10,754-10,777*Nucleotide...

Báo cáo khoa học: "SOFA is superior to MOD score for the determination of non-neurologic organ dysfunction in patients with severe traumatic brain injury: a cohort study" doc

... Alberta, Canada2Department of Clinical Neuroscience, University of Calgary, Calgary, Alberta, Canada3Department of Medicine, University of Calgary, Calgary, Alberta, Canada4Department of Community ... Health Sciences, University of Calgary, Calgary, Alberta, Canada5Department of Surgery, University of Calgary, Calgary, Alberta, CanadaCorresponding author: David Zygun, david.zygun@calgaryhealthregion.caReceived: ... ICU for each day of theirICU stay and mortality status. Ethical review and approval wasattained from the regional ethics review board.In the Calgary Health Region, adult trauma services are...

Báo cáo hóa học: "Biocompatible micro-sized cell culture chamber for the detection of nanoparticle-induced IL8 promoter activity on a small cell population" pot

... size of cavities enables a high number of cav-ities on each chip and facilitates the performance of many independent assays on one plate. The funnel-shaped cavities avoid the appearance of meniscuses, ... in eachcultivation segment guarantee a statistical analysis of the generated data. The MCC represents an array of miniaturized cell culture chambers for permanent non-invasive characterization ... using the forward primer 5’-ata ctcgag ggg tac ctt cgt cat act ccg tat ttg ata agg aac a- 3’and the reverse primer 5’-aga att cgc ata gat ctt ccg gtggtt tct tcc tgg ctc tt-3’, containing the...

Báo cáo khoa học: " Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection" ppsx

... apical staining of this marker wasapparent in distinct areas of 3-D Huh7 aggregates (Fig. 3).Taken together, these data demonstrate that the expres-sion and distribution of cell adhesion and TJ ... study and participated in drafting the manu-script. All authors read and approved the final manu-script.AcknowledgementsWe thank Drs. Heather L. LaMarca and Kerstin Hönzer zu Bentrup for helpful ... transport in choroid plexus. J Biol Chem1987, 262:13907-13915.37. Nakata K, Tanaka Y, Nakano T, Adachi T, Tanaka H, Kaminuma T,Ishikawa T: Nuclear receptor-mediated transcriptional regu-lation...

Báo cáo khoa học: "Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection" pdf

... cellsurface ectopeptidase that localizes to the apical plane of polarized cells [45], was non-detectable in 2-D Huh7monolayers, while, apical staining of this marker wasapparent in distinct areas of ... transport in choroid plexus. J Biol Chem1987, 262:13907-13915.37. Nakata K, Tanaka Y, Nakano T, Adachi T, Tanaka H, Kaminuma T,Ishikawa T: Nuclear receptor-mediated transcriptional regu-lation ... Morphological andcytological examination of cultures demonstrated thatHuh7 cells efficiently adhered to the collagen-coatedmicrocarrier beads and that these individual beads thenassembled to form...

Báo cáo hóa học: " Quinoline Group Modiﬁed Carbon Nanotubes for the Detection of Zinc Ions" ppt

... and the carboxyl groups available on the surface of the support. Fourier transform infraredspectroscopy (FTIR) and Thermogravimetric analysis(TGA) unambiguously proved the existence of covalentbonds ... was just based on organic molecules asﬂuorescent chemosensors. For a few practical applications the attachment of the ﬂuorescent units to a solid supporthas advantages like the possibility of ... MWNTs. A new peak that appeared around1686 cm-1is attributed to the amide carbonyl (C=O)stretch. Another new peak at 1629.87 cm-1is attributed tosecondary amide band which accompanies the absorptionat...

Báo cáo y học: "Computerized two-lead resting ECG analysis for the detection of coronary artery stenosis" pps

... Mathematical transformations of ECG data The 3DMP ECG analysis employs six mathematical transformations. All these transformations are based on the power spectrum of the recorded ECG leads ... Asia, and North America on individuals of varying ages and degrees of disease state including normal populations [17,18]. All ECG analyses in this database have been validated against the ... generate indexes of abnormality. The resulting patterns of the indexes are then compared for abnormality to the patterns in the reference database to reach a final diagnostic output. In addition...

Báo cáo y học: " Computerized two-lead resting ECG analysis for the detection of coronary artery stenosis after coronary revascularization" doc

... RJ, Abrams J, Chatterjee K, et al. ACC/AHA 2002 guideline update for the management of patients with chronic stable angina summary article: a report of the American College of Cardiology/American ... Beasley JW, et al. ACC/AHA guideline update for the management of patients with unstable angina and non-ST-segment elevation myocardial infarction 2002: summary article: a report of the American ... Harris RA, et al. The role of coronary angiography and coronary revascularization before noncardiac vascular surgery. JAMA. 1995; 273: 1919-1925. 8. Scanlon PJ, Faxon DP, Audet AM, et al. ACC/AHA...

Báo cáo y học: "Monoclonal Antibodies against Nucleophosmin Mutants: Potentials for the Detection of Acute Myeloid Leukemia" ppt

... Hematology, University of Perugia, Perugia, Italy). The forward and backward primers were: 5’-CGGGATCCATCGAAGGTCGTGAAGATTCGATGGACAT-3’, and 5’-CGCGCGACCGAGCGGAA GCTTCTATTTTCTTAAAGAGAC-3’. Underlined ... clinical diagnosis/prognosis of NPMc+AML, we need to prepare the anti-NPM-mA monoclonal antibodies (mAbs) as the primary antibody in IHC assay. In 1999, Cordell et al prepared the first panel of ... 12. Vardiman JW, Thiele J, Arber DA, et al. The 2008 revision of the World Health Organization (WHO) classification of myeloid neoplasms and acute leukemia: rationale and important changes....

Báo cáo khoa học: Nautilin-63, a novel acidic glycoprotein from the shell nacre of Nautilus macromphalus doc

... mono-saccharidic composition. The composition of neutral sugars isobtained by HPAE-PAD. Data are represented as ngÆlg)1 of the total matrix and as a percentage of the total identiﬁed carbohydratecompounds. ... CaCO3precipitation. First, the effect of the nacre matrix started to occur above 1 lg of the ASM and the delay of the reaction was dose-dependent. At approximately 50 lg of nacre ASM, a complete ... microstructure self-assembly, are ﬁnally occluded either between the super-imposed parallel lamellae (‘interlamellar matrix’), at the boundary of adjacent mature nacre tablets (‘intertabu-lar matrix’),...

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